Find research datasets worth reusing

Fig. 6 in Mobilization of storage lipid reserve and expression analysis of lipase and lipoxygenase genes in rice (Oryza sativa var. Pusa Basmati 1) bran during germination

Fig. 6. Expression analysis of rice bran lipase genes using quantitative RT PCR (qRT PCR) during 2 HAI, 1, 2, 4, 6, and 8 DAI. Expression value at 0 HAI was taken as control while elongation factor 1-alpha (EF1A, accession no.: XM_015774317.2) was used as the reference gene to normalize the expression level of lipases used in this study. Bars represent means ± standard deviation (SD) of 3 biological replicates. HAI, hours after imbibition; DAI, days after imbibition.

Fig. 5 in Mobilization of storage lipid reserve and expression analysis of lipase and lipoxygenase genes in rice (Oryza sativa var. Pusa Basmati 1) bran during germination

Fig. 5. Schematic representation of the conserved motifs in (a) OsLip proteins and (b) OsLOX proteins. Each colored box represents a motif in the respective protein sequence. Motif number and color codes indicate the sequence of the conserved motif. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 in Mobilization of storage lipid reserve and expression analysis of lipase and lipoxygenase genes in rice (Oryza sativa var. Pusa Basmati 1) bran during germination

Fig. 1. Lipid and FA content in bran of germinating rice grains. (a) Lipid content in bran tissues (b) FA profiles of TAGs (c) FA profiles of DAGs (d) FA profiles of NEFAs and (e) FA profiles of PLs. TAG, Triacylglycerol; DAG, diacylglycerol; NEFA, non-esterified fatty acid; PL, polar lipid. Results are mean values ± SD of three replicates.

Fig. 7 in Mobilization of storage lipid reserve and expression analysis of lipase and lipoxygenase genes in rice (Oryza sativa var. Pusa Basmati 1) bran during germination

Fig. 7. Expression analysis of rice bran LOX genes using quantitative RT PCR (qRT PCR) during 2 HAI, 1, 2, 4, 6, and 8 DAI. Expression value at 0 HAI was taken as control while elongation factor 1-alpha (EF1A, accession no.: XM_015774317.2) was used as the reference gene to normalize the expression level of LOXs used in this study. Bars represent means ± standard deviation (SD) of 3 biological replicates. HAI, hours after imbibition; DAI, days after imbibition.

Fig. 4 in Mobilization of storage lipid reserve and expression analysis of lipase and lipoxygenase genes in rice (Oryza sativa var. Pusa Basmati 1) bran during germination

Fig. 4. Exon/intron organization of (a) OsLip genes [Exons (green boxes), Introns (red lines)] and (b) OsLOX genes [Exons (pink boxes), Introns (grey lines)]. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 3 in Mobilization of storage lipid reserve and expression analysis of lipase and lipoxygenase genes in rice (Oryza sativa var. Pusa Basmati 1) bran during germination

Fig. 3. Phylogenetic analysis of rice LOXs. Neighbor-joining (NJ) estimates (with 1000 bootstrap replicates) the phylogenetic relationship between rice and Arabidopsis LOX proteins. Labels are depicted in green for the 9-LOX clade, in blue for 13-LOX clade. Bootstrap values are mentioned in the branches. Branch length is indicated by the scale bar. The Arabidopsis Information Resource (TAIR) and Rice genome annotation project (RGAP) accession numbers are indicated with species names of the lipase proteins. Os, Oryza sativa; At, Arabidopsis thaliana. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2 in Mobilization of storage lipid reserve and expression analysis of lipase and lipoxygenase genes in rice (Oryza sativa var. Pusa Basmati 1) bran during germination

Fig. 2. Phylogenetic analysis of rice lipases (Lip). Neighbor-joining (NJ) estimates (with 1000 bootstrap replicates) the phylogenetic relationship between rice and Arabidopsis lipase proteins. AtLip are depicted in green box and six OsLip candidates selected for the study based on the tree are depicted in blue box. Bootstrap values are mentioned in the branches. Branch length is indicated by the scale bar. The Arabidopsis Information Resource (TAIR) and Rice genome annotation project (RGAP) accession numbers are indicated with species names of the lipase proteins. Os, Oryza sativa; At, Arabidopsis thaliana; SDP1, sugar dependent1. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 7 in Phylogeny, structural diversity and genome-wide expression analysis of fibrillin family genes in rice

Fig. 7. Expression profiles analysis of OsFBNs under different temperature stresses. Eleven OsFBN genes in rice were detected for their transcript levels in leave tissues of seedlings at the three-leaf stage with different time intervals after the 35 ̊C (heat stress) and 6 ̊C (cold stress) stresses in the growth chamber, separately. The OsUBQ gene was used as a normalization control; data was measured as the mean values ± SD of three replicates, respectively.

Fig. 4 in Phylogeny, structural diversity and genome-wide expression analysis of fibrillin family genes in rice

Fig. 4. Phylogenetic and exon-intron structure analysis of FBN family genes in rice and Arabidopsis. The phylogenetic analysis was performed using the amino acid sequences of 25 FBN family proteins in rice and Arabidopsis (Left). The unrooted phylogenetic tree was generated by the neighbor-joining method and displayed using MEGA7 software. Numbers above or below branches of the evolution tree indicated the bootstrap values; The exon-intron structures of 25 FBN family genes in rice and Arabidopsis were analyzed (Right), the exons were represented by yellow boxes, the UTR by blue, black lines connecting two exons were introns. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 6 in Phylogeny, structural diversity and genome-wide expression analysis of fibrillin family genes in rice

Fig. 6. The in vitro lipid binding assays of PAP-domains. A and C, schematic representations of the mutant residues in OsFBN2 and OsFBN4 proteins in the in vitro lipid binding assays. B and D, the in vitro lipid-binding activity assays were performed by a fluorescence lipid probe P-96 (1 mM), fatty acids with C12:0, C14:0, C16:0, C18:0, C20:0, C22:0, with each (0.5 mM) of wild type (Wt) and mutant fusion protein (Mut) of PAP domains in OsFBN2 and OsFBN4 proteins, respectively. Detection experiments were performed with three biological replicates. * and ** symbols indicated the significant difference levels of P <0.05 or P <0.01 between mutant and wild type, respectively.

Fig. 5 in Phylogeny, structural diversity and genome-wide expression analysis of fibrillin family genes in rice

Fig. 5. Subcellular localization of CTP domains-fused GFP proteins. These constructs of p35S-FBN1-11-CTP-GFP or p35S-Trxm5-GFP, separately, were transiently expressed in Nicotiana benthamiana mesophyll cells. The co-localizations of fluorescence patterns of CTP domains of ten OsFBN proteins except OsFBN8 fused GFP were observed with the autofluorescence of chlorophyll in chloroplasts in protoplasts. The Trxm5-GFP was used as a chloroplast marker.

Fig. 3 in Phylogeny, structural diversity and genome-wide expression analysis of fibrillin family genes in rice

Fig. 3. PKC domain analysis of FBN11 clade in plants. A, Homology analysis of PKC domain sequences in FBN11 clades; B, evolutionary tree analysis of PKC domain in five plant species (Oryza sativa (Os), Selaginella moellendorffii (Smo), Arabidopsis thaliana (At), Glycine max (Gm), and Zea mays (Zm)) and algae (Monoraphidium neglectum (Mon), Chlorella sorokiniana (Chlo), Chlamydomonas sp. UWO 241 (Chla), and Haemato coccus lacustris (Hae); C, the conserved motif analysis of PKC domain in FBN11 protein clades in higher plants.

Fig. 2 in Phylogeny, structural diversity and genome-wide expression analysis of fibrillin family genes in rice

Fig. 2. Box-plot graphs and motif analysis of CTP and PAP-domain in plants. The protein sequences of CTP domains and PAP domains were analyzed in six plant species: Selaginella moellendorffii (Smo), Physcomitrella patens (Ppa), Arabidopsis thaliana (At), Glycine max (Gm), Oryza sativa (Os), and Zea mays (Zm), The grand average of hydropathicity (GRAVY) and isoelectric point (pI) of CTP domains of FBN1-11 protein clades were showed in A and B, respectively; The GRAVY and pI of PAP domains in FBN1-11 protein clades were showed in C and D, respectively; The dotted line represented the average values of GRAVY and pI, respectively; The conserved motif sequence of PAP domains in these plants were showed in E.

Fig. 1 in Phylogeny, structural diversity and genome-wide expression analysis of fibrillin family genes in rice

Fig. 1. Phylogenetic tree analysis of 121 fibrillin family proteins from 10 representative species. The neighbor-joining tree was constructed using the sequences of 121 fibrillin family proteins in ten representative species: Synechococcus sp. PCC 7002 (Ssp), Erysimum siliculosus (Esi), Emiliania huxleyii (Ehu), Thalassiosira pseudonana (Tps), Chlamydomonas reinhardtii (Cre), Volvox carteri (Vca), Physcomitrella patens (Ppa), Selaginella moellendorffii (Smo), Arabidopsis thaliana (At) and Oryza sativa (Os). Bootstrap values were indicated on the branches. Each fibrillin protein was labeled by its name (Table S3). The green and red color meant the proteins present in algae or terrestrial plant, respectively; the blue color represents the proteins present in both algae and terrestrial plants. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 4 in Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia'

Fig. 4. Product analysis of LoTPS4 produced from GGPP and FPP. (a) GC-MS analysis (ion chromatogram) of the LoTPS4 enzyme product from GGPP. (b) GC-MS analysis (ion chromatogram) of products obtained from assays with LoTPS4 and FPP. (c, d, e, f) Mass spectra of Peak 1, Peak 2, Peak 3 and Peak 4. Di-epi-α-cedrene (Peak 1), α-cubebene (Peak 2): trans-α-bergamotene (Peak 3) and (E)-β-farnesene (Peak 4).

Fig. 3 in Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia'

Fig. 3. (a) GC-MS analysis of products produced from assays with extracts of the empty vector and GPP. (b) Analysis (total ion chromatogram) of the product of the LoTPS4 enzyme generated from GPP (c) Total ion chromatogram of the D-limonene authentic standard. (d) Mass spectrum of the Peak 3 (e) Mass spectrum of Dlimonene in the floral scent of Lilium 'Siberia' (f) Mass spectrum of the D-limonene authentic standard (g, h, I and j) Mass spectra of Peak 1, Peak 2, Peak 4 and Peak 5. β-Phellandrene (Peak 1), β-myrcene (Peak 2), D-limonene (Peak 3), 3-Carene (Peak 4) and (+)-4-Carene (Peak 5).

Fig. 10 in Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia'

Fig. 10. Subcellular localization of LoTPS2 and LoTPS4. Confocal laser scanning microscopy of LoTPS2 and LoTPS4 was performed by using EGFP fusion proteins in Arabidopsis protoplasts. The full-length coding region and the N-terminal 80 amino acids of the coding region of LoTPSs were fused to the GFP reporter gene in the p35 S vector to generate the p35 S-LoTPSs/GFP construct. The red column shows chlorophyll autofluorescence; the green column shows GFP fluorescence; the merged columns show combined GFP fluorescence and chlorophyll autofluorescence; and the BF columns represent bright field images. The names of the constructs are given on the left. HcTPS7 from H. coronarium was used as a marker. Scale bars: 5 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2 in Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia'

Fig. 2. GC-MS analysis of products generated by LoTPS2. (a) GC-MS analysis (total ion chromatogram) of the products obtained by incubating extracts of the empty vector (control) with geranyl pyrophosphate. (b) GC-MS analysis (total ion chromatogram) of the LoTPS2 enzyme with GPP. (c) GC–MS analysis (total ion chromatogram) of the LoTPS2 enzyme with farnesyl pyrophosphate showing (E, E)-α- farnesene as the sole product. (d) Mass spectrum of the peak. (e) Mass spectrum of (E, E)-α-farnesene in the NIST08 library.

Fig. 7 in Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia'

Fig. 7. Relative expression analysis of LoTPS2 and LoTPS4 during different flower development performed by quantitative real-time PCR. (a, b) Transcript levels of the LoTPS2 and LoTPS4 genes during flower development. (c, d) Relative expression analysis of LoTPS2 and LoTPS4 at different flower developmental stages. Flower development was divided into 5 different stages: D1 (bud stage), D2 (little open), D3 (half open), D4 (full-bloom), D5 (senescence). GAPDH was used as an internal control. The root was set as 1. Data are presented as the mean ± SEM (n = 3).

Fig. 8 in Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia'

Fig. 8. Relative expression analysis of LoTPS genes and the emission of (E, E)-α-farnesene and D-limonene from Lilium 'Siberia' at different time intervals. (a, b) Expression pattern of LoTPS2 and LoTPS4 for 3 days postanthesis. (c) Emission pattern of (E, E)-α-farnesene from Lilium 'Siberia' (d) Emission pattern of D-limonene from Lilium 'Siberia' flowers over 3 days after full-bloom. The plants were kept under a 12 h light, 12 h dark photoperiod. GAPDH was used as an internal control. Each point is the average of 3 replicates.