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111,247 results for “cells”
scGPT: End-to-End Protocol for Fine-tuned Retina Cell Type Annotation
<h1>Abstract</h1> <p>Single-cell research faces challenges in accurately annotating cell types at high resolution, especially when dealing with large-scale datasets and rare cell populations. To address this, foundation models like scGPT offer flexible, scalable solutions by leveraging transformer-based architectures. This protocol provides a comprehensive guide to fine-tuning scGPT for cell-type classification in single-cell RNA sequencing (scRNA-seq) data. We demonstrate how to fine-tune scGPT on a custom retina dataset, highlighting the model’s efficiency in handling complex data and improving annotation accuracy achieving 99.5% F1-score. This protocol automates key steps, including data preprocessing, model fine-tuning, and evaluation. This protocol enables researchers to efficiently deploy scGPT for their own datasets. The provided tools, including a command-line script and Jupyter Notebook, simplify the customization and exploration of the model, proposing an accessible workflow for users with minimal Python and Linux knowledge. The protocol offers an off-the-shell solution of high-precision cell-type annotation using scGPT for researchers with intermediate bioinformatics.</p>
Cell-free DNA Fragmentomics and Second Malignant Neoplasm Risk in Patients with PTEN Hamartoma Tumor Syndrome
<p><strong>Supplementary dataset for manuscript:</strong></p> <p><strong>Cell-free DNA Fragmentomics and Second Malignant Neoplasm Risk in Patients with PTEN Hamartoma Tumor Syndrome</strong></p> <p>Affiliations:</p> <p><sup>1</sup>Genomic Medicine Institute, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA</p> <p><sup>2</sup>Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH 44195, USA</p> <p><sup>3</sup>Rose Ella Burkhardt Brain Tumor and Neuro-Oncology Center, Cleveland Clinic, Cleveland, OH 44195, USA</p> <p><sup>4</sup>Center for Personalized Genetic Healthcare, Medical Specialties Institute, Cleveland Clinic, Cleveland, OH 44195, USA</p> <p><sup>5</sup>Center for Immunotherapy and Precision Immuno-oncology, Cleveland Clinic, Cleveland, OH 44195, USA</p> <p><sup>6</sup>Taussig Cancer Institute, Cleveland Clinic, Cleveland, OH 44195, USA</p> <p><sup>7</sup>Department of Genetics and Genome Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA</p> <p><sup>8</sup>Germline High Risk Cancer Focus Group, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106, USA</p> <p><strong><em>Correspondence</em></strong>:</p> <p>Darren Liu, MD, MS (<a href="https://orcid.org/0000-0002-6703-1843">https://orcid.org/0000-0002-3693-5145)</a></p> <p>Cleveland Clinic Genomic Medicine Institute</p> <p>9500 Euclid Avenue, Cleveland, OH 44195, USA</p> <p>E-mail: liud5@ccf.org </p>
Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs
<p>Supplementary data and videos for the manuscript 'Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs', by Çevrim,<sup> </sup>Laplace-Builhé,<sup> </sup>Sugawara, Rusciano, Labert, Brocard, Almazán and Averof.</p> <p>The supplementary data include:</p> <p><strong>Supplementary Data 1 (.csv file); Live imaging of regenerating <em>Parhyale</em> legs: image acquisition settings</strong></p> <p>Table with information on the 22 time lapse recordings presented in Figure 3, including image acquisition settings, temperature and duration of the recordings.</p> <p><strong>Supplementary Data 2 (.zip file); Live imaging of regenerated <em>Parhyale</em> legs: maximum projections</strong></p> <p>Compressed folder including maximum projections for each of the 22 time lapse recordings presented in Figure 3. These files were generated by projecting all or a subset of the z slices acquired at each time point. A 20 micron scale bar was added on the first time point. These files serve as a quick way to examine the 22 time lapse recordings.</p> <p><strong>Supplementary Data 3 (22 .tif files); Live imaging of regenerated <em>Parhyale</em> legs: complete datasets</strong></p> <p>Complete image 3D+T hyperstacks for each of the 22 time lapse recordings presented in Figure 3. These files have been generated by concatenating the original image stacks and correcting any image shifts, as described in the Methods section of the paper.</p> <p><strong>Supplementary Data 4 (.zip file); Analysis of trade-offs of imaging resolution and image quality</strong></p> <p>The data used for the analysis of trade-offs in imaging and the results shown in Table 1 are included in this compressed folder. Folders for the original recording (labelled 00), for each of the subsampled datasets (labelled 01 to 05), and for the denoised and deconvoluted datasets each include the corresponding image data and ground truth cell tracking files (.tif, .h5, .xml and .mastodon files) and three sets of cell track predictions (.mastodon files). There are also separate folders containing the Elephant detection and flow model parameters for each set of predictions.</p> <p dir="ltr"><strong>Supplementary Data 4 (.zip file); Analysis of trade-offs of imaging resolution and image quality</strong></p> <p dir="ltr">The data used for the analysis of trade-offs in imaging and the results shown in Table 1 are included in two folders. The folder named Image_and_tracking_data includes the image data (.tif, .h5, .xml), ground truth cell tracking files (.mastodon files) and three sets of cell track predictions (.mastodon files) for the original recording (labelled 00), for each of the subsampled datasets (labelled 01 to 05), and for the denoised and deconvoluted datasets. It also includes separate folders containing the Elephant detection and flow model parameters for each set of predictions. The folder named CTC_tracking_results includes the ground-truth data along with three sets of predictions for detection and tracking for each dataset, following the Cell Tracking Challenge format. For each dataset we include label image files (.tif) for every time point along with tracking results in .txt format, and each results directory (01_RES_*) also contains the evaluation results from the Cell Tracking Challenge Evaluation Software. For a detailed explanation of the folder structure, please refer to the Cell Tracking Challenge documentation.</p> <p><strong>Supplementary Data 5 (.zip file); Tracking the progenitors of spineless-expressing cells in the distal carpus</strong></p> <p>The data used to generate Figure 7 are included in this compressed folder, including the live imaging and cell tracking files (.h5, .xml and .mastodon files) and the image stack of the spineless and futsch HCR and DAPI stainings (.tif file). Channel 2 shows spineless expression (mostly nascent transcripts in nuclei), as well as background signal in epidermal nuclei (possibly due to photoconversion of DAPI, see Karg & Golic 2018, Chromosoma 127: 235-245) and strong autofluorescence in granular cells (also visible in channel 1, depicting futsch HCR).</p> <p><strong>Supplementary Data 6 (.txt file); Sequences of <em>Parhyale</em> genes targeted by the HCR probes</strong></p> <p>The sequences are provided in FASTA format.</p> <p dir="ltr"><strong>Supplementary Data 7 (.zip file); Apoptosis in legs that have not been subjected to live imaging</strong></p> <p dir="ltr">The data used to generate Figure 2 supplement 2 are contained in this compressed folder, including 9 image stacks of T4 and T5 legs fixed and stained with DAPI 3 days post amputation (with apoptotic nuclei marked) and a .txt file containing the apoptotic cell counts.</p> <p dir="ltr"><strong>Supplementary Data 8 (.zip file); Analysis of tracking performance in relation to imaging depth</strong></p> <p dir="ltr">The data used to generate Figure 5 are contained in this compressed folder, including separate folders for the data extracted from the analysis of datasets #1 to #5. Each folder includes data from three replicates (batches 001 to 003), with .csv files listing the z location of nucleus centroids (in µm) for the nuclei that were incorrectly detected by Elephant – either as false positives (FP) or as false negatives (FN) – and the ground truth data (GT). The folder also includes an .xlsx file gathering all the relevant data and the measurements of precision and recall.</p> <p dir="ltr"><strong>Supplementary Data 9 (.zip file); Detecting the temporal pattern of cell divisions in regenerating legs</strong></p> <p dir="ltr">The data used to generate Figure 4 are contained in this compressed folder, including the five image datasets (.tif, .h5, .xml), the detected cell divisions (.mastodon files), and an .xlxs file containing all the cell divisions counts and graphs.</p> <p><strong>Video 1. Time lapse recording of regeneration in a Parhyale T5 leg (dataset li48-t5)</strong></p> <p>Live imaging of nuclei labelled with H2B-mREFruby (maximum projection of z slices 3-10). Proximal parts of the leg are to the left and the amputation site is at the right of the frame. For annotations of different features please refer to Figure 2. Shortly after leg amputation (0 hpa) hemocytes adhere to the wound. By 16 hpa the wound has melanized. Up to ~32 hpa epithelial cells can be seen migrating and accumulating at the wound, below the melanized scab (Figure 2A,B). Around 31 hpa, the leg tissues become detached from the scab (Figure 2C). At 43 hpa, the carpus-propodus boundary first becomes visible, and thereafter many cells can be observed dividing at the distal part of the leg stump (Figure 2D). At 56 hpa, the propodus-dactylus boundary first becomes visible (Figure 2E). At later stages, tissues in more proximal parts of the leg retract, making space for the regenerating leg to grow (Figure 2F,G). After ~90 hpa cell proliferation there is less cell proliferation and cell movements, and the nuclear positions within the tissue become fixed. Scale bars, 20 µm.</p> <p><strong>Video 2. Time lapse recording of regeneration in a Parhyale T5 leg (dataset li36-t5)</strong></p> <p>Live imaging of nuclei labelled with H2B-mREFruby (maximum projection of z slices 3-15). Proximal parts of the leg are to the left and the amputation site is at the right of the frame. The sequence of events is similar to that described in Video 1, but the progression is slower: epithelial migration towards the wound is observed up to 40 hpa, tissues detach from the scab at 65 hpa, and the carpus-propodus and propodus-dactylus boundaries first become visible at 78 and 91 hpa. The tissues making up the carpus and propodus can be seen pulsating from 105 to 145 hpa. Scale bars, 20 µm.</p>
Additional Data - Phototoxicity induced in living HeLa cells by focused femtosecond laser pulses
<p>Nonlinear optical microscopy is a powerful label-free imaging technology, providing biochem-ical and structural information in living cells and tissues. A possible drawback is photodamageinduced by high-power ultrashort laser pulses. Here we present an experimental study on thou-sands of HeLa cells, to characterize the damage induced by focused femtosecond near-infraredlaser pulses as a function of laser power, scanning speed and exposure time, in both wide-field andpoint-scanning illumination configurations. Our data-driven approach offers an interpretation ofthe underlying damage mechanisms and provides a predictive model that estimates its probabilityand extension and a safety limit for the working conditions in nonlinear optical microscopy. Inparticular, we demonstrate that cells can withstand high temperatures for a short amount of time,while they die if exposed for longer times to mild temperatures. It is thus better to illuminatethe samples with high irradiances: thanks to the nonlinear imaging mechanism, much strongersignals will be generated, enabling fast imaging and thus avoiding sample photodamage.</p>
Dynamics of CTCF and cohesin mediated chromatin looping revealed by live-cell imaging
<p><strong>Overview</strong></p> <p>This repository contains all the raw and processed trajectory data associated with “paper title”. In this ReadMe file we provide the following information:</p> <ul> <li>The cell lines and conditions used in this study</li> <li>A summary of how the data was collected</li> <li>The structure of the chromosome locus tracking data</li> </ul> <p><strong>Cell lines and conditions</strong></p> <p>In total, the dataset covers 12 experimental conditions representing the following cell lines and treatment conditions:</p> <ul> <li>C36</li> <li>C65</li> <li>C27</li> <li>CTCF-AID (untreated)</li> <li>CTCF-AID (2 hours AID)</li> <li>CTCF-AID (4 hours AID)</li> <li>RAD21-AID (untreated)</li> <li>RAD21-AID (2 hours AID)</li> <li>RAD21-AID (4 hours AID)</li> <li>WAPL-AID (untreated)</li> <li>WAPL-AID (4 hours AID)</li> <li>WAPL-AID (6 hours AID)</li> </ul> <p> </p> <p><strong>Data and data processing</strong></p> <p>Trajectories were obtained from 3D timeseries of mouse embryonic stem cell colonies in the conditions listed above using a LSM900 Airyscan 2 Zeiss microscope. For each movie we recorded 365 frames of 49.69 µm x 49.69 µm (584 x 584 pixels, pixel size: 0.085 µm by 0.085 µm), separated by an interval of 20 seconds for a total of just over 2 hours. 3D images were composed of 30 z-stacks separated by 0.25 µm, for a total height of 7.25 µm. Imaging was performed in two colors allowing the tracking of two arrays of fluorophores on Chromosome 18 near the <em>Fbn2</em> gene. In all conditions, the fluorophore arrays were separated by 515 kb (except the C27 clone where separation was 10 kb).</p> <p>The 3D image time series were processed using ConnectTheDots: <a href="https://github.com/ahansenlab/connect_the_dots">https://github.com/ahansenlab/connect_the_dots</a> to obtain paired trajectories of chromosome loci over time. The trajectories have been corrected for chromatic shifts and aberrations.</p> <p>Data are provided in an “unfiltered” format (meaning that individual dot localizations were not quality control filtered) , or a filtered format (the same data set, but having undergone quality control). The filtered (quality controlled) trajectory data was used for all the quantitative analyses in the article “”.</p> <p>File names are formatted follows.</p> <ul> <li>Quality controlled data have the structure: {Clone_and_condition_name}.tagged_set.tsv</li> <li>Unfiltered data have the structure: {Clone_and_condition_name}.unfiltered.tagged_set.tsv</li> </ul> <p>For example, for RAD21-AID tagged clone, for imaging performed after two hours of protein degradation, the quality-controlled file name is: RAD21_2_hr.tagged_set.tsv. Please note that for all no-treatment conditions, we used “0 hours” as the tag. Thus, the RAD21 (untreated) becomes RAD21_0_hr.tagged_set.tsv.</p> <p> </p> <p><strong>Structure of Data</strong></p> <p>The trajectory data are provided as tab-separated text files consisting of 10 columns. The column headers are:</p> <ul> <li>id: a unique dot pair index</li> <li>t: the frame in which the dots were localized</li> <li>x: x-coordinate of the dot in the EGFP channel (units in µm)</li> <li>y: y-coordinate of the dot in the EGFP channel (units in µm)</li> <li>z: z-coordinate of the dot in the EGFP channel (units in µm)</li> <li>x2: x-coordinate of the dot in the mScarlet channel (units in µm)</li> <li>y2: y-coordinate of the dot in the mScarlet channel (units in µm)</li> <li>z2: z-coordinate of the dot in the mScarlet channel (units in µm)</li> <li>dist: 3D distance between the dots across channels (units in µm)</li> <li>movie_index: an identifier used to link the dot pair back to the raw image timeseries.</li> </ul>
Data for: Epicardial slices: an innovative 3D organotypic model to study epicardial cell physiology and activation.
<p>Raw data set for Npj Regenerative Medicine article: Epicardial slices: an innovative 3D organotypic model to study epicardial cell physiology and activation.</p>
Xiao et al, Oligodendrocyte Precursor Cells Sculpt the Visual System by Regulating Axonal Remodeling [Dataset]
<p>Raw data from the behavior and imaging experiments of Xiao et al., Nature Neuroscience 2022. For additional details about the acquisition of each part of the dataset, refer to the methods section of the paper. From this dataset, using the published code, all the figures relative to the imaging in the optic tectum can be generated and all the cumulative statistics for the behavioural assays run with Stytra recomputed.</p> <p> </p> <p><strong>Organisation of the dataset</strong></p> <p>This dataset is organised in the following subdirectories:<br> - <em>freely_swimming</em>: contains the data for the freely swimming experiments quantifying motor activity in the various ablated groups. It contains subfolders of groups, each of which contains the Stytra raw data directories for all fish of that group. Refer to `Stytra` and `bouter` documentation for further details about the files.<br> - <em>OMR</em>: contains the data for the quantification of OMR reflex across different spatial frequencies. It contains subfolders for the control and ablated group, each of which contains the Stytra raw data directories for all fish of that group.<br> - <em>receptive_field_imaging</em>: contains the imaging data for the receptive field estimation. Subfolders contains, for each individual fish (both ablated and controls are pooled in the same directory):<br> - stytra raw output from the experiment<br> - data_from_suite2p_unfiltered.h5: `flammkuchen`-loadable `.h5` file that contains the raw fluorescent trace<br> - anatomy.mask: `flammkuchen`-loadable mask file saved by the `pypra` tool that was used for segmenting the tectum, delimiting the region of the tectum<br> The folder contains an additional file, `manual_alignment_offsets.h5`, where the offsets of the manual morphing across fish were saved.</p>
Automatic labelling of HeLa "Kyoto" cells using Deep Learning tools
<p><strong>Name</strong>: Automatic labelling of HeLa “Kyoto” cells using Deep Learning tools</p> <p><strong>Data type</strong>: Microscopy images from the dataset “<strong>HeLa “Kyoto” cells under the scope</strong>”, Brightfield (BF), Digital Phase Contrast (DPC, either “raw” or “square-rooted”), Tubulin and H2B fluorescent channel, paired with their corresponding nuclei or cell/cyto label images.</p> <p><strong>Labels images</strong>: Labels images were generated using the script <em>“prepare_trainingDataset_cellpose.ijm</em>”.</p> <p>Briefly, for 5 defined time-points (1,10,50,100,150), channels of interest were duplicated, resaved and :</p> <p>- nuclei label images were obtained using <a href="https://github.com/stardist/stardist">StarDist</a> on H2B channel</p> <p>- cell label images were obtained using <a href="https://github.com/MouseLand/cellpose">Cellpose</a> on Tubulin and H2B channels</p> <p>A quick visual inspection of the resulting label images concluded that they were satisfying enough, despite certainly not being perfect.</p> <p>Notes :</p> <p>- This labelling strategy:</p> <p>o will not produce 100% accurate labels, but they might be more reproducible than labels generated by humans and are (definitely) much faster to obtain.</p> <p>o is <strong>NOT a recommended way of generating labels images</strong>, but for educational purposes.</p> <p>- The fluorescent channels are part of the dataset to ease the process of review of the labels and are NOT used for training. We generated the labels from the fluorescent channels to later predict labels from the BF or DPC channels only. As such, the fluorescent channels should not be “reused” with our labels during training.</p> <p><strong>File format</strong>: .tif (16-bit)</p> <p><strong>Image size</strong>: 540x540 (Pixel size: 0.299 nm)</p> <p> </p> <p><strong><em>NOTE</em></strong>: This dataset uses the “HeLa “Kyoto” cells under the scope” dataset (<a href="https://doi.org/10.5281/zenodo.6139958">https://doi.org/10.5281/zenodo.6139958</a>) to automatically generate annotations</p> <p><strong><em>NOTE</em></strong>: This dataset was used to train cellpose models in the following Zenodo entry <a href="https://doi.org/10.5281/zenodo.6140111">https://doi.org/10.5281/zenodo.6140111</a></p>
HeLa "Kyoto" cells under the scope
<p><strong>Name</strong>: HeLa “Kyoto” cells under the scope</p> <p><strong>Microscope</strong>: Perkin Elmer Operetta microscope with a 20x N.A. 0.8 objective and an Andor Zyla 5.5 camera.</p> <p><strong>Microscopy data type</strong>: The time-lapse datasets were acquired every 15 minutes, for 60 hours. From the individual plan images (channels, time-points, field of view exported by the PerkinElmer software Harmony) multi-dimension images were generated using the <a href="https://github.com/BIOP/ijp-operetta-importer/releases/tag/Operetta_Importer-0.1.21">Operetta_Importer-0.1.21</a> with a downscaling of 4. </p> <p>Channel 1 : Low Contrast DPC (Digital Phase Contrast)</p> <p>Channel 2 : High Contrast DPC</p> <p>Channel 3 : Brightfield</p> <p>Channel 4 : EGFP-α-tubulin</p> <p>Channel 5 : mCherry-H2B</p> <p><strong>File format</strong>: .tif (16-bit)</p> <p><strong>Image size</strong>: 540x540 (Pixel size: 0.299 nm), 5c, 1z , 240t</p> <p> </p> <p><strong>Cell type</strong>: HeLa “Kyoto” cells, expressing EGFP-α-tubulin and mCherry-H2B ( <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839080/">Schmitz <em>et al</em>, 2010</a> )</p> <p><strong>Protocol</strong>: Cells were resuspended in <strong>Imaging media</strong> and were seeded in a microscopy grade 96 wells plate ( <a href="https://catalyse-erm.epfl.ch/erd-client/app/secure/sourcesearch/results?p=1&channel=SpendDirectorCatalog">CellCarrier Ultra 96</a>, Perkin Elmer). The day after seeding, and for 60 hours, images were acquired in 3 wells, in 25 different fields of view, every 15 minutes.</p> <p><strong>Imaging media</strong>: DMEM red-phenol-free media (FluoroBrite™ DMEM, Gibco) complemented with Fetal Calf Serum and Glutamax.</p> <p> </p> <p><strong>NOTE: </strong>This dataset was used to automatically generate label images in the following Zenodo entry: <strong> <a href="https://doi.org/10.5281/zenodo.6140064">https://doi.org/10.5281/zenodo.6140064</a></strong></p> <p><strong>NOTE: </strong>This dataset was used to train the cellpose models in the following Zenodo entry:<strong> <a href="https://doi.org/10.5281/zenodo.6140111">https://doi.org/10.5281/zenodo.6140111</a></strong></p>
Efficient embryoid-based method to improve generation of optic vesicles from human induced pluripotent stem cells data
<p>Animal models have provided many insights into ocular development and disease, but they remain suboptimal for understanding human oculogenesis. Eye development requires spatiotemporal gene expression patterns and disease phenotypes can differ significantly between humans and animal models, with patient-associated mutations causing embryonic lethality reported in some animal models. The emergence of human induced pluripotent stem cell (hiPSC) technology has provided a new resource for dissecting the complex nature of early eye morphogenesis through the generation of three-dimensional (3D) cellular models. By using patient-specific hiPSCs to generate <em>in vitro </em>optic vesicle-like models, we can enhance the understanding of early developmental eye disorders and provide a pre-clinical platform for disease modelling and therapeutics testing. A major challenge of <em>in vitro </em>optic vesicle generation is the low efficiency of differentiation in 3D cultures. To address this, we adapted a previously published protocol of retinal organoid differentiation to improve embryoid body formation using a microwell plate. Established morphology, upregulated transcript levels of known early eye-field transcription factors and protein expression of standard retinal progenitor markers confirmed the optic vesicle/presumptive optic cup identity of <em>in vitro </em>models between day 20 and 50 of culture. This adapted protocol is relevant to researchers seeking a physiologically relevant model of early human ocular development and disease with a view to replacing animal models.</p>
Control T-cell receptor (TCR) alpha and beta chain nucleotide and amino acid sequences from human and mouse
<p>A dataset of pooled T-cell receptor (TCR) sequences for TCR alpha and beta chains of human and mouse.</p> <p>Sequences are obtained from various samples of healthy individuals/mice using our conventional protocols: see for example [Britanova et al "Dynamics of individual T cell repertoires: from cord blood to centenarians" The Journal of Immunology 2016] and [Izraelson et al. "Comparative analysis of murine T‐cell receptor repertoires." Immunology 2018].</p> <p>The sequences are stored as gzipped clonotype tables in VDJtools format, see [https://vdjtools-doc.readthedocs.io/en/master/input.html#vdjtools-format].</p> <p>This control dataset can be used as a proxy for a generative VDJ rearrangement model to estimate the expected frequency distribution of TCRs and check for enrichment of rare TCR clonotypes and groups of similar TCR sequences. For the implementation of the enrichment analysis, please see CalcDegreeStats routine from VDJtools software, see [https://vdjtools-doc.readthedocs.io/en/master/annotate.html#calcdegreestats].</p> <p>Files named "human.tra.strict.txt.gz", etc are pools of random/naive TCR clonotypes containing unique V/J/CDR3 nucleotide sequence combinations observed in data. The pools.zip file is used for TCR motif inference in VDJdb database [https://github.com/antigenomics/vdjdb-motifs], it contains human.tra.aa.txt, etc files that contain random/naive TCR clonotypes grouped by CDR3 amino acid sequence with the most frequent representative V and J.</p>
Single-cell analyses of axolotl forebrain organization, neurogenesis, and regeneration
<p>Preprint: https://doi.org/10.1101/2022.03.21.485045</p> <p>Abstract:</p> <p>Salamanders are important tetrapod models to study brain organization and regeneration, however the identity and evolutionary conservation of brain cell types is largely unknown. Here, we delineate cell populations in the axolotl telencephalon during homeostasis and regeneration, representing the first single-cell genomic and spatial profiling of an anamniote tetrapod brain. We identify glutamatergic neurons with similarities to amniote neurons of hippocampus, dorsal and lateral cortex, and conserved GABAergic neuron classes. We infer transcriptional dynamics and gene regulatory relationships of postembryonic, region-specific direct and indirect neurogenesis, and unravel conserved signatures. Following brain injury, ependymoglia activate an injury-specific state before reestablishing lost neuron populations and axonal connections. Together, our analyses yield key insights into the organization, evolution, and regeneration of a tetrapod nervous system.</p> <p> </p> <p>File description:</p> <p>all_nuclei_clustered_highlevel_anno.rds - Seurat object including all snRNA-seq data from uninjured pallium, both from microdissections and whole pallium multiome.</p> <p>pallium_metadata_simp.csv - csv file containing a simplified version of the metadata for the uninjured pallium</p> <p>Edu_1_2_4_6_8_12_fil_highvarfeat.rds - Seurat object containing all Div-seq data for the pallium injury time course</p> <p>divseq_predicted_metadata.csv - csv file containing a simplified version of the metadata for the pallium injury time course</p> <p>ep_wpi_srat.rds - Seurat object containing an integrated version of ependymoglia cells from uninjured and injured pallium (see Fig 6 in the preprint).</p> <p>D1_113_sub_b.rds - Seurat object containing a Visium data for the axolotl pallium</p> <p>multiome_integATAC_SCT.rds - Signac object containing the data used for multiome analysis of the uninjured whole pallium</p> <p>predictions_cell2loc.csv - csv file containing cell2location scores for the uninjured pallium cell types in the Visium dataset</p>
A Single-Cell Tumor Immune Atlas for Precision Oncology
<p><strong>Publication version of the Single-Cell Tumor Immune Atlas</strong></p> <p>This upload contains:</p> <ul> <li><strong>TICAtlas.rds:</strong> an rds file containing a Seurat object with the whole Atlas</li> <li><strong>TICAtlas.h5ad:</strong> an h5ad file with the whole Atlas</li> <li><strong>TICAtlas_downsampled.rds:</strong> an rds file containing a downsampled version of the Seurat object of the whole Atlas</li> <li><strong>TICAtlas_downsampled.h5ad:</strong> an rds file containing a downsampled version of the Seurat object of the whole Atlas</li> <li><strong>TICAtlas_metadata.csv: </strong>a comma-separated text file with the metadata for each of the cells</li> </ul> <p>All the files contain the following patient/sample metadata variables:</p> <ul> <li>patient: assigned patient identifiers</li> <li>nCountRNA and nFeatureRNA: number of UMIs and genes per cell</li> <li>percent.mt: percentage of mitochondrial genes</li> <li>gender: the patient's gender (male/female/unknown)</li> <li>source: dataset of origin</li> <li>subtype: cancer type (abbreviations as indicated in the preprint)</li> <li>kmeans_cluster: patients clusters, NA if filtered out before clustering</li> <li>lv1 and lv2: annotated cell type for each of the cells, two level annotation (lv2 has more cell types)</li> </ul> <pre> </pre> <p>If you have any issues with the metadata (i.e. unexpected factors, NA values...) you can use the <strong>TICAtlas_metadata.csv </strong>file.</p> <p>For more information, <a href="https://genome.cshlp.org/content/early/2021/09/21/gr.273300.120.">read our paper</a>, <a href="https://github.com/Single-Cell-Genomics-Group-CNAG-CRG/Tumor-Immune-Cell-Atlas">check our GitHub</a> and our <a href="https://singlecellgenomics-cnag-crg.shinyapps.io/TICA/">ShinyApp</a>.</p> <p>h5ad files can be read with Python using <a href="https://scanpy.readthedocs.io/en/stable/">Scanpy</a>, rds files can be read in R using <a href="https://satijalab.org/seurat/">Seurat</a>. For format conversion between AnnData and Seurat we recommend <a href="https://mojaveazure.github.io/seurat-disk/">SeuratDisk</a>. For other single-cell data formats you can use <a href="https://github.com/cellgeni/sceasy">sceasy</a>.</p>
Zellige example dataset: primary culture of human bronchial cells infected by SARS-CoV-2
<p>A human bronchial epithelium was infected by SARS-CoV-2. The specimen was imaged four days post-infection. The z-stack image encompasses the very irregular epithelium surface. It was acquired with a point scanning microscope (Zeiss LSM700) equipped with Zeiss Plan-Apochromat 63x lens (NA=1.4). Pixel size 0.110µm, z step 0.4µm. This dataset contains both the ground-truth height map and the height map generated with Zellige. The Zellige parameters used are: <span class="math-tex">\(T_{A}=23, T_{otsu}=16, S_{min}=5, \sigma_{xy}=4, \sigma_{z}=1, T_{OSE1}=0.9, R_{1}=5, C_{1}=0.9, T_{OSE2}=0.1, R_{2}=5, C_{2}=0.8.\)</span>.</p> <p>Nota: to compare the ground truth height map with the Zellige height map, one first needs to substrat 1 to all values of the Zellige height map.</p> <p>See the related paper:<br> <a href="https://hal-pasteur.archives-ouvertes.fr/pasteur-03319522">https://hal-pasteur.archives-ouvertes.fr/pasteur-03319522</a></p> <p>See the accompanying paper: Extracting multiple surfaces from 3D microscopy images in complex biological tissues with the Zellige software tool. Trébeau <em>et al.</em> 2022: <a href="https://doi.org/10.1101/2022.04.05.485876">https://doi.org/10.1101/2022.04.05.485876</a></p> <p> </p>
Data for "Transient Density-Induced Dipolar Interactions in a Thin Vapor Cell"
<p>Data for "Transient Density-Induced Dipolar Interactions in a Thin Vapor Cell" as .npz files. The "datasets.csv" file lists, where each dataset is used in the journal publication. Additionally, there are two Python scripts (.py) which show how to load and plot a dataset. The dataset can be selected by changing the file name string in the script. Note, that for loading and plotting the simulation dataset the "plot_simulation.py" script has to be used.</p>
Evaluating the influence of structural properties on proximity metric performance in single cell RNA-seq data - Datasets
<p>Includes raw and processed copies of the scRNA-seq datasets used for the paper: '<strong>How does data structure impact cell-cell similarity? Evaluating the influence of structural properties on proximity metric performance in single cell RNA-seq data.'</strong></p> <p><strong>Real scRNA-seq.zip </strong>contains the Abundant (subset1) and Rare (subset 2) subsets generated to represent discretely structured datasets (sourced from<strong> </strong> Wegmann et al. 2019) and the continuously structured data (sourced from Popescu et al. 2019).</p> <p><strong>Simulated scRNA-seq.zip</strong> contains the Abundant, Moderately-Rare and Ultra-Rare subsets for discretely and continuously structured datasets. All data was simulated using the PROSSTT package in Python 3.8, as well as the dataset containing the labels to re-produce Figure 3 of the manuscript.</p> <p><strong>Results.zip </strong>contains the results for all datasets from the full analysis, in a pickled python dictionary. Code to read in and visualise results is available on the projects github</p> <p>The scripts for the dataset generation, processing and visualisation of results are available at <a href="https://github.com/Ebony-Watson/scProximitE">our github for the scProcimitE package</a>, and documentation is available <a href="https://ebony-watson.github.io/scProximitE/">here</a>.</p>
NanoString dataset for study: Impairment of cancer-associated fibroblasts promotes CD8+ T cell infiltration and enhances sensitivity to immune checkpoint blockade
<p>Pre-processed NanoString mRNA abundance data and associated sample sheet for study:</p> <p>Impairment of cancer-associated fibroblasts promotes CD8+ T cell infiltration and enhances sensitivity to immune checkpoint blockade</p>
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes
<p>Raw multichannel and/or Z-stack source data from time series images in TIF format to accompany publication of:</p> <p><strong>Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes</strong></p> <p>Jina Ko<sup>1</sup>, Martin Wilkovitsch<sup>2</sup>, Juhyun Oh<sup>1</sup>, Rainer Kohler<sup>1</sup>, Evangelia Bolli<sup>1,3</sup>, Mikael J. Pittet<sup>1,3,4,5</sup>, Claudio Vinegoni<sup>1</sup>, David B. Sykes<sup>6,7</sup>, Hannes Mikula<sup>2</sup>, Ralph Weissleder<sup>1,8</sup>*, Jonathan C. T. Carlson<sup>1,7</sup>*</p> <p><sup>1 </sup>Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114 </p> <p><sup>2</sup> Institute of Applied Synthetic Chemistry, TU Wien, 1060 Vienna, Austria </p> <p><sup>3</sup> Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland</p> <p><sup>4</sup> Ludwig Institute for Cancer Research, Lausanne Branch, Switzerland</p> <p><sup>5</sup> AGORA Cancer Center, Lausanne, Switzerland</p> <p><sup>6</sup> Center for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA</p> <p><sup>7 </sup>Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA</p> <p><sup>8 </sup>Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115</p>
Geografisches hexagonales Gitter mit 1 Quadratkilometer Zellengröße für Deutschland - Geographical hexagonal grid with one square kilometer cell size for Germany
<p>Ein räumliches Gitter stellt eine abstrakte Definition von einheitlich großen Bezugszellen für statistische Auswertungen dar. Dieses macht Auswertungen und Vergleiche gegenüber räumlichen Abgrenzungen auf der Basis von administrativen Grenzen wie Kreisen oder Gemeinden unterschiedlicher Größen einfacher. Geografische Gitterdefinitionen werden eingesetzt, um eine vordefinierte Raumbezugsstruktur zu haben mit einer einheitlicheren Verteilung von Zellen in der Fläche.</p> <p><br> Ein Gitter mit sechseckigen, eben hexagonalen Zellen bietet eine Alternative zu rechteckigen statistischen Gittern wie das GeoGitter vom Bundesamt für Kartographie und Geodäsie (BKG, 2020). Ein Hexagon-Gitter bietet neben der Eigenschaft homogene Einheiten für statistische Analysen zu bilden (Schindler et al. 2008) mit sechs unmittelbaren Nachbarzellen vorteilhaftere Voraussetzungen für Nachbarschaftsanalysen im Vergleich zu quadratischen Zellen mit vier Kantennachbarn (White et al. 1992). Als Beispiel werden im Bild 1 mittels Hexagonen-Gitter Volumenunterschiede der aufragenden Vegetation pro Zelle dargestellt.</p>
PhasAGE Training School 2 - Phase separations and transitions by viral proteins: from viral factories to interference with host cell functions- LECTURE
<p>The Training School 2 “Biomolecular condensates in cell function, aging and disease” is the <strong>second</strong> edition of a series of PhasAGE training activities.</p> <p> </p> <p>The main goal of this training school is to raise awareness and provide expertise on fundamental aspects of phase separation and formation of <strong>biomolecular condensates</strong>, specifically covering the importance of this process to cellular biology and its contribution to the aging process and age-related diseases.</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.