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3,597 results for “Genomic data”
Data in support of 'Mechanistic insights into plant community responses to environmental variables: genome size, cellular nutrient investments, and metabolic trade-offs.'
Data was collected to examine whether and how the plant genome size (GS) influences traits (stomata size, stomata density, cellular and tissue level carbon (C), nitrogen (N), and phosphorus (P) contents) and metabolic-tradeoffs (of photosynthesis, evapotranspiration, water-use, efficiency) of plants in treatment plots in which nothing, N, P, or NP had been annually added. Data was collected from ~500 plants from seven grassland sites that are all part of the Nutrient Network (https://nutnet.org), a globally distributed experiment in which plots have different nutrient amendment treatments that are administered identically to allow cross-site comparisons of the effects of nutrients on biodiversity patterning. The sites chosen varied along a North-South latitude, longitude, mean annual precipitation (MAP) and mean annual temperature (MAT) gradient.
Genome-Wide DNA Methylation in Peripheral Blood and Long-Term Exposure to Source-Specific Transportation Noise and Air Pollution: The SAPALDIA Study (Supplementary Data)
<p>The zip file contains supplementary data for the publication - Genome-Wide DNA Methylation in Peripheral Blood and Long-Term Exposure to Source-Specific Transportation Noise and Air Pollution: The SAPALDIA Study, accepted for publication in Environmental Health Perspectives (DOI: 10.1289/EHP6174).</p> <p>The description of the files are noted below:</p> <p><strong>1. Readme File for SAPALDIA Noise and Air Pollution EWAS Single Exposure.zip </strong></p> <p>This zip file contains all the results of the association between source-specific transportation noise (aircraft, railway and road traffic), air pollution (NO<sub>2</sub> and PM<sub>2.5</sub>), and genome-wide DNA methylation, derived from multi-exposure models.</p> <p><strong>SAPALDIA_EWAS_SingleExposure_AircraftLden.txt</strong> contains the results for aircraft noise</p> <p><strong>SAPALDIA_EWAS_SingleExposure_RailwayLden.txt</strong> contains the results for railway noise</p> <p><strong>SAPALDIA_EWAS_SingleExposure_RoadtrafficLden.txt</strong> contains the results for road traffic noise</p> <p><strong>SAPALDIA_EWAS_SingleExposure_NO2.txt</strong> contains the results for nitrogen dioxide</p> <p><strong>SAPALDIA_EWAS_SingleExposure_PM25.txt</strong> contains the results for fine particulate matter</p> <p> </p> <p><strong>General footnote for all files:</strong>SAPALDIA: Swiss cohort study on air pollution and lung and heart diseases in adults. CpG: Cytosine-phosphate-Guanine. CHR: chromosome. SE: standard error. Lden: day-evening-night noise level. NO<sub>2</sub>: nitrogen dioxide. PM<sub>2.5</sub>: particulate matter with aerodynamic diameter <2.5 µm. Beta coefficients represent increase or decrease in DNA methylation per 10 dB increase in aircraft, railway or road traffic Lden or 10 µg/m<sup>3</sup> increase in NO<sub>2</sub> or PM<sub>2.5</sub>. All estimates were from single exposure epigenome-wide linear mixed models, with random intercept at the level of participant. Each model was adjusted for age, sex, educational level, area, and neighborhood socio-economic status, greenness index, smoking status and pack years, exposure to passive smoke, consumption of fruits, vegetables and alcohol, nested study, asthma status, survey, source-specific noise truncation indicator (for Lden models) and leukocyte composition. In a preliminary step, DNA methylation β-values were regressed on the Illumina control probe-derived first 30 principal components to correct for correlation structures and technical bias, and residuals of these regressions covering 430,477 CpGs were used as the technical bias-corrected methylation level at the CpG sites.</p> <p>Extreme values of the residuals (lying beyond three times the interquartile range below the first quartile and above the third quartile at each CpG site) were replaced with their corresponding detection threshold value (“modified winsorization”). The “winsorized” data were then used as the dependent variables in the epigenome-wide association study.</p> <p> </p> <p><strong>2. Readme File for SAPALDIA Noise and Air Pollution EWAS Multi Exposure.zip </strong></p> <p>This zip file contains all the results of the association between source-specific transportation noise (aircraft, railway and road traffic), air pollution (NO<sub>2</sub> and PM<sub>2.5</sub>), and genome-wide DNA methylation, derived from multi-exposure models.</p> <p><strong>SAPALDIA_EWAS_MultiExposure_AircraftLden.txt</strong> contains the results for aircraft noise</p> <p><strong>SAPALDIA_EWAS_MultiExposure_RailwayLden.txt</strong> contains the results for railway noise</p> <p><strong>SAPALDIA_EWAS_MultiExposure_RoadtrafficLden.txt</strong> contains the results for road traffic noise</p> <p><strong>SAPALDIA_EWAS_MultiExposure_NO2.txt</strong> contains the results for nitrogen dioxide</p> <p><strong>SAPALDIA_EWAS_MultiExposure_PM25.txt</strong> contains the results for fine particulate matter</p> <p><strong>General table footnotes: </strong>SAPALDIA: Swiss cohort study on air pollution and lung and heart diseases in adults. CpG: Cytosine-phosphate-Guanine. CHR: chromosome. SE: standard error. Lden: day-evening-night noise level. NO<sub>2</sub>: nitrogen dioxide. PM<sub>2.5</sub>: particulate matter with aerodynamic diameter <2.5 µm. Beta coefficients represent increase or decrease in DNA methylation per 10 dB increase in aircraft, railway or road traffic Lden or 10 µg/m<sup>3</sup> increase in NO<sub>2</sub> or PM<sub>2.5</sub>. All estimates were from multi-exposure epigenome-wide linear mixed models, with random intercept at the level of participant, and were adjusted for age, sex, educational level, area, and neighborhood socio-economic status, greenness index, smoking status and pack years, exposure to passive smoke, consumption of fruits, vegetables and alcohol, nested study, asthma status, survey, source-specific noise truncation indicator and leukocyte composition. Multi-exposure models included all five exposures (Aircraft, railway, road traffic Lden and respective truncation indicators, NO<sub>2</sub> and PM<sub>2.5</sub>) at the same time. In a preliminary step, DNA methylation β-values were regressed on the Illumina control probe-derived first 30 principal components to correct for correlation structures and technical bias, and residuals of these regressions covering 430,477 CpGs were used as the technical bias-corrected methylation level at the CpG sites. Extreme values of the residuals (lying beyond three times the interquartile range below the first quartile and above the third quartile at each CpG site) were replaced with their corresponding detection threshold value (“modified winsorization”). The “winsorized” data were then used as the dependent variables in the epigenome-wide association study.</p>
Variant, Metabolite and Source Data for: Population genomics uncover loci for trait improvement in the indigenous African cereal tef (Eragrostis tef)
<p>These files contain the variant and metabolome for a collection of 220 tef (<em>Eragrsotis tef)</em> accessions from an ethiopian diversity panel. The accessions were assembled and managed by the Ethiopian Institute of Agricultural Research (EIAR, Ethiopia). The variant data was produced at the John Innes Centre (UK). The metabolome data was produced at Aberystwyth University (UK). These dataset are described in Jones et al. (2024), <em>bioRxiv</em>, https://doi.org/10.1101/2024.09.30.615331. The source data for main figures in the publication are also included.</p> <p>The submission contains</p> <ol> <li>EIAR_filtered.vcf.gz: This is the variant data obtained from alignment of Illumina reads from all 220 teff accessions to the reference assembly of tef (Dabbi). Low quality variants were filtered out. This variant data was used for constructing the phylogenetic relationship between the accessions. The samples names corresponds to the DNA code in Supplementary Table S10 (Jones et al, 2024).</li> <li>pooled_EIAR_filtered.vcf.gz: After the phylogentic analysis described above, reads from accessions that were found to be genetically redundant were pooled before variant calling. This file was used for the SNP GWAS analysis. The samples names corresponds to the DNA code in Supplementary Table S10 (Jones et al, 2024).</li> <li> Metabolite_Profile.xlxs (source data for Figure 5): This file contains m/z feature intensities from untargeted metabolite fingerprinting using Flow Infusion Electrospray High-resolution Mass Spectrometry (FIE-HRMS). The sample names contains a combination of Location code and Plot number in Supplementary Table S10 e.g AT plot 1, CD plot 1, DZ plot 1, where AT, CD and DZ represent Alem Tena, Chefe Donsa and Debre Zeit, respectively. The data was used for the partial least squares discriminant analysis and differentially accumulated metabolites analysis presented in Figure 5.</li> <li>Source data: Numerical source data for graphs and charts in Figures 3 - 7.</li> <li>Tsedey TT2 Sequence from Improved Assembly: The 4A and 4B sequences around the TT2 orthologue in tef from the improved PacBio-based chromosome-scale assembly of tef. These sequences were used for plotting the LTR Copia alignments presented in Supplementary Figure 9. We thank Corteva for pre-publication access to this improved Tsedey genome assembly.</li> </ol>
Supplementary data for draft genome of a member of the ascomycotal fungal genus Pseudopithomyces (family Didymosphaeriaceae)
<p><span lang="EN-US">We update our previous draft genome of a member of genus <em>Pseudopithomyces</em> (previously annotated as <em>Pseudopithomyces maydicus</em> strain SBW1, now reannotated as <em>Pseudopithomyces sp</em>. strain SBW1. The new draft genome is based on a hybrid assembly utilising both ONT and Illumina data. The draft genome is comprised of 43 contigs with a total length of 39.65Mbp. We predict 13,669 protein coding gene models, of which 4241 (31%) were annotated to KEGG Orthology. Taxonomic assignment to <em>Pseudopithomyces sp.</em> was supported by comparative analysis of extracted ITS regions, mitochondrial DNA sequence and whole genome comparisons using <em>k</em>-mer sketches. </span></p> <p> </p> <p><span lang="EN-US">The following items of Additional Data Files are made available in this repository:</span></p> <p><span lang="EN-US">Additional Data File 1: contigs.fasta</span></p> <p><span lang="EN-US">FASTA file of entire assembly. </span><span lang="EN-US"> </span></p> <p> </p> <p><span lang="EN-US">Additional Data File 2: draft_genome.fasta</span></p> <p><span lang="EN-US">FASTA file of draft whole genome sequence.</span></p> <p> </p> <p><span lang="EN-US">Additional Data File 3: ITS_full.fasta</span></p> <p><span lang="EN-US">FASTA file containing full length ITS sequences from contig 23 and contig 42.</span></p> <p><span lang="EN-US"> </span></p> <p><span lang="EN-US">Additional Data File 4: 2NJ47W4U013-Alignment.txt</span></p> <p><span lang="EN-US">Text file containing BLASTN alignments for ITS region contained on contig 23</span></p> <p><span lang="EN-US"> </span></p> <p><span lang="EN-US">Additional Data File 5: 2NJXH2GE013-Alignment.txt</span></p> <p><span lang="EN-US">Text file containing BLASTN alignments for ITS region contained on contig 42</span></p> <p><span lang="EN-US"> </span></p> <p><span lang="EN-US">Additional Data File 6: 2NM79EUG016-Alignment.txt</span></p> <p><span lang="EN-US">Text file containing BLASTN alignments for the mitochondrial genome from contig 40 </span></p> <p><span lang="EN-US"> </span></p> <p><span lang="EN-US">Additional Data File 6: sourmash_bc10_hy_pm1_3.txt</span></p> <p><span lang="EN-US">Text file containing the MASH similarities of the draft genome compared to 18,883 fungal genomes.</span></p>
Grapegenomics.com: a web portal with genomic data and analysis tools for wild and cultivated grapevines
<p><a href="https://grapegenomics.com">Grapegenomics.com</a> is a web portal that provides public access to genome references for grapevine cultivars (<em>Vitis vinifera</em> ssp. <em>vinifera</em>), wild grapevines (<em>Vitis vinifera</em> ssp. <em>sylvestris</em>), various wild grape species (<em>Vitis</em> spp. and <em>Muscadinia</em> spp.), and major fungal pathogens affecting grapes.</p> <p>All genomes are accessible through dedicated genome browsers, and published genomes are available for complete <a href="https://www.grapegenomics.com/download.php">download</a>.</p> <p>The site hosts all genomes produced by the laboratory of Dario Cantù in the Department of Viticulture and Enology at the University of California, Davis, along with published genome references generated by others, such as PN40024 and Pinot noir ENTAV115. Instructions for genome submission are provided <a href="https://www.grapegenomics.com/submit.php">here</a>. The portal is maintained by Noé Cochetel (ndcochetel[at]ucdavis.edu). In this version 2.0, all genome browsers utilize <a href="https://jbrowse.org/jb2/">jbrowse 2</a>. <br><br>Link to the website: <a href="https://www.grapegenomics.com">https://www.grapegenomics.com</a> </p>
Genome data from Effrenium voratum CCMP421, RCC1521, and rt-383 and their analysis
<p>This dataset represents secondary data generated from the genomic analysis of three isolates of <em>Effrenium voratum</em> (CCMP421, RCC1521, and rt-383), the early-diverging, free-living lineage of Symbiodiniaceae dinoflagellates. Theese data include <strong>(A)</strong> assembled genome sequences, predicted gene models and protein sequences, and <strong>(B)</strong> data and scripts associated with generation of graphs and figures presented in the key genome paper (Shah et al., 2024, Massive genome reduction predates the divergence of Symbiodiniaceae dinoflagellates, under review in <em>ISME Journal</em>). An earlier preprint of this manuscript is available at <em>bioRxiv</em>: <a href="https://doi.org/10.1101/2023.03.24.534093" target="_blank" rel="noopener">https://doi.org/10.1101/2023.03.24.534093</a>.</p> <p><strong>A. Genome assemblies, annotation and gene models. </strong>The dataset includes, for each taxon, (a) the <em>de novo</em> assembled genome sequences in FASTA format (<strong>*genome.fa.tgz</strong>), (b) structural annotation of the assembled genome in GFF3 format (<strong>*.genome.annotation.gff3.tgz</strong>), (c) the predicted protein-coding sequences of gene models in FASTA format (<strong>*genemodel.CDS.fa.tgz</strong>), (d) the predicted protein sequences of gene models in FASTA format (<strong>*genemodel.PROT.fa.tgz</strong>), and (c) the associated sequences and gene annotations of organellar genomic sequences (i.e. mitochondrial and plastid) (<strong>*organellar.tgz</strong>). Functional annotations of all gene models from the three genomes are available in the Excel spreadsheet (<strong>*GeneModels.xlsx</strong>).</p> <p><strong>B. Data and scripts associated with generation of graphs and figures in Shah et al. (2024). </strong>These files are organised based on key analyses specific to main figures and supplementary figures in the paper.</p> <p>See <strong>README.txt</strong> for a more-detailed description of the files.</p>
Frictionless Tabular Data Package for GC-MS data from the 'Rose Genome' article published in Nature genetics, June, 2018
<p>This dataset, in the form of a Frictionless Tabular Data Package (<a href="https://frictionlessdata.io/specs/tabular-data-package/">https://frictionlessdata.io/specs/tabular-data-package/)</a>, holds the measurements of 61 known metabolites (all annotated with resolvable CHEBI identifiers and InChi strings), measured by gas chromatography mass-spectrometry (GC-MS) in 6 different Rose cultivars (all annotated with resolvable NCBITaxonomy Identifiers) and 3 organism parts (all annotated with resolvable Plant Ontology identifiers). The quantitation types are annotated with resolvable <a href="https://github.com/ISA-tools/stato">STATO</a> terms. </p> <p>The data was extracted from a supplementary material table, available from <a href="https://static-content.springer.com/esm/art%3A10.1038%2Fs41588-018-0110-3/MediaObjects/41588_2018_110_MOESM3_ESM.zip">https://static-content.springer.com/esm/art%3A10.1038%2Fs41588-018-0110-3/MediaObjects/41588_2018_110_MOESM3_ESM.zip</a> and published alongside the Nature Genetics manuscript identified by the following doi: <a href="https://doi.org/10.1038/s41588-018-0110-3">https://doi.org/10.1038/s41588-018-0110-3</a>, published in June 2018. This supplementary material table was deposited to Zenodo and is identified by the following doi: <a href="https://doi.org/10.5281/zenodo.2598799">https://doi.org/10.5281/zenodo.2598799</a></p> <p>This dataset is used to demonstrate how to make data Findable, Accessible, Discoverable and Interoperable (FAIR) and how Frictionless Tabular Data Package representations can be easily mobilised for reanalysis and data science.</p> <p>It is associated to the following project: <a href="https://github.com/proccaserra/rose2018ng-notebook">https://github.com/proccaserra/rose2018ng-notebook</a> with all the necessary information, executable code and tutorials in the form of Jupyter notebooks.</p>
Frictionless Tabular data package for GC-MS data from Rose Genome article published in Nature genetics, June, 2018
<p>This dataset, in the form of a Frictionless Tabular Data Package (https://frictionlessdata.io/specs/tabular-data-package/), holds the measurements of 61 known metabolites (all annotated with resolvable CHEBI identifiers and InChi), measured by gas chromatography mass-spectrometry (GC-MS) in 6 different Rose cultivars (all annotated with resolvable NCBITaxId) and 3 organism parts (all annotated with resolvable Plant Ontology identifiers). The data was extracted from a supplementary material table, available from https://static-content.springer.com/esm/art%3A10.1038%2Fs41588-018-0110-3/MediaObjects/41588_2018_110_MOESM3_ESM.zip and published alongside the Nature Genetics manuscript identified by the following doi: https://doi.org/10.1038/s41588-018-0110-3, published in June 2018. This dataset is used to demonstrate how to make data Findeable, Accessible, Discoverable and Interoperable(FAIR) and how Tabular Data Package representations can be easily mobilized for re-analysis and data science. It is associated to the following project available from github at: https://github.com/proccaserra/rose2018ng-notebook with all necessary information and Jupyter notebooks.</p>
Supplementary dataset to publication: Oxford nanopore technologies - a valuable tool to generate whole-genome sequencing data for in silico serotyping and the detection of genetic markers in Salmonella, Thomas et al 2023
<p>Bacteria of the genus <em>Salmonella</em> pose a major risk to livestock, the food economy, and public health. <em>Salmonella</em> infections are one of the leading causes of food poisoning. The identification of serovars of <em>Salmonella</em> achieved by their diverse surface antigens is essential to gain information on their epidemiological context. Traditionally, slide agglutination has been used for serotyping. In recent years, whole-genome sequencing (WGS) followed by <em>in silico</em> serotyping has been established as an alternative method for serotyping and the detection of genetic markers for <em>Salmonella</em>. Until now, WGS data generated with Illumina sequencing are used to validate <em>in silico</em> serotyping methods. Oxford Nanopore Technologies (ONT) opens the possibility to sequence ultra-long reads and has frequently been used for bacterial sequencing. In this study, ONT sequencing data of 28 <em>Salmonella</em> strains of different serovars with epidemiological relevance in humans, food, and animals were taken to investigate the performance of the <em>in silico</em> serotyping tools SISTR and SeqSero2 compared to traditional slide agglutination tests. Moreover, the detection of genetic markers for resistance against antimicrobial agents, virulence, and plasmids was studied by comparing WGS data based on ONT with WGS data based on Illumina. Based on the ONT data from flow cell version R9.4.1, <em>in silico</em> serotyping achieved an accuracy of 96.4 and 92% for the tools SISTR and SeqSero2, respectively. Highly similar sets of genetic markers comparing both sequencing technologies were identified. Taking the ongoing improvement of basecalling and flow cells into account, ONT data can be used for <em>Salmonella in silico</em> serotyping and genetic marker detection.</p>
Geochemical, physicochemical, and genomic data from a continental-scale survey of microbial diversity in Antarctic soils (2003-2023)
This data package offers comprehensive insights into Antarctic soil microbial diversity and composition. From 2003 to 2023, a total of 186 samples were collected from diverse locations spanning the Antarctic Peninsula to East Antarctica, representing a wide range of environmental gradients and climatic conditions. Soils were stored at -20°C to preserve their integrity for downstream analyses. This data package integrates cultivation-independent sequencing of prokaryotic and fungal communities alongside a robust cultivation-dependent culture collection to enable direct comparisons across microbial diversity assessment methods. Accompanying geochemical, physicochemical, and environmental parameters provide critical context for biogeographical analyses, offering a valuable resource for studying microbial adaptations and community dynamics in extreme Antarctic environments.
Supplementary data for Willemsen et al., 2024 "Novel high-quality amoeba genomes reveal widespread codon usage mismatch between giant viruses and their hosts".
<p>Supplementary data for Willemsen et al., 2024 "Novel high-quality amoeba genomes reveal widespread codon usage mismatch between giant viruses and their hosts". The data set consists of five folders: “Codon_usage_amoebae_and_viruses”, "Genome_annotations_amoebae", "Phylogenetic_trees_18S_amoebae", “Phylogenomic_trees_amoebae”, and "Viral_integration_detection_amoebae". The “Codon_usage_amoebae_and_viruses” folder contains for each amoeba host the calculated codon usage tables in the subfolder "codon_usage_table_host", the calculated codon usage preferences using different scores in the subfolder "codon_usage_scores_host", and the calculated codon usage preferences of giant viruses versus each host in the subfolder "codon_usage_scores_viruses_vs_host". The giant viruses in the subfolder "codon_usage_scores_viruses_vs_host" are organised by viral family and genus in separate sub-subfolders. The "Genome_annotations_amoebae" folder contains the generated genome annotations in different formats and the manually curated mitochondrial genome annotations for each amoeba host. The "Phylogenetic_trees_18S_amoebae" contains for the eukaryotic phyla <em>Discosea</em>, <em>Heterolobosea</em>, and <em>Tubulinea, </em>the 18S rRNA nucleotide alignments, distance matrices, and computed phylogenetic trees. The folder "Phylogenomic_trees_amoebae" contains for the eukaryotic clades <em>Amoebozoa</em> and <em>Discoba, </em>the protein alignment matrices and computed phylogenomic trees. The folder "Viral_integration_detection_amoebae" contains the MCP databases used (fasta file, alignment file, HMM profile and DIAMOND BLASTX database) and the MCP sequences detected in this study and the blast results of these. </p>
GWAS Summary Statistics for Publication: Identifying novel genetic and phenotypic associations to genomic features by leveraging off-target reads in exome sequencing data
<p>This dataset contains summary statistics for genome-wide association studies (GWAS) conducted on genomic features derived from off-target reads in whole-exome sequencing (WES) data. The study utilized tools like Seeing Beyond the Target (SBT) and ImReP to construct novel phenotypic features from unmapped reads in ~50,000 participants in the UK Biobank. Features include mitochondrial DNA (mtDNA) copy number, ribosomal DNA (rDNA) copy number (5S, 18S, 28S), immune repertoire metrics (e.g., T-cell receptor alpha diversity), and microvial genome load (viral and fungal).</p> <p>Summary statistics can be used for replication studies, meta-analyses, or further exploration of these phenotypes.</p>
Data for manuscript: A framework for integrating genomics, microbial traits, and ecosystem biogeochemistry
<p>Support manuscript: A framework for integrating genomics, microbial traits, and ecosystem biogeochemistry. </p> <p>Dataset includes 1) model and analysis, and 2) supplemental data. </p> <p>In the "model_analysis" file, we include the ecosys model source code, the modeling runs, and the modeling results. The detailed introduction is in README.md file. </p> <p><strong>Acknowledgments</strong></p> <p>We thank the EMERGE Biology Integration Institute Coordinators (members listed in Supplementary Information) for project guidance and management. This research is a contribution of the EMERGE Biology Integration Institute, funded by the National Science Foundation, Biology Integration Institutes Program, Award # 2022070 (V.I.R., R.K.V., S.R.S., M.B.S., E.L.B., and the EMERGE Coordinators). Additional support for individual contributors included the following. Z.L. was additionally supported by Lawrence Livermore National Laboratory under the auspices of the U.S. Department of Energy under contract DE-AC52-07NA27344. W.J.R. was supported by the Belowground Biogeochemistry Scientific Focus Area and U.K. was supported by the Watershed Function Science Area, both funded by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research under contract no. DE-AC02-05CH11231. G.L.M. was supported by the LLNL "Microbes Persist" Soil Microbiome Scientific Focus Area SCW1632 and an associated KBase award SCW1746. N.J.B. was supported by the US Department of Energy, Office of Science (BER), Early Career Research Program (#FP00005182). B.J.W. was supported by an Australian Research Council Future Fellowship (#FT210100521). J.T. was supported by the Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory. </p> <p>We thank the Swedish Polar Research Secretariat and SITES for the support of the work done at the Abisko Scientific Research Station. SITES is supported by the Swedish Research Council’s grant 4.3-2021-00164. This research used resources of the National Energy Research Scientific Computing Center (NERSC) which is a U.S. Department of Energy Office of Science user facility. This research used the Lawrencium computational cluster resource provided by the IT Division at the Lawrence Berkeley National Laboratory (Supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231). </p> <p><strong>Full list of the EMERGE Biology Integration Institute Coordinators and Affiliations</strong></p> <p>Eoin L. Brodie1,2, Sarah C. Bagby3, Jeffrey P. Chanton4, Jessica G. Ernakovich5, Regis Ferriere6,7, Suzanne B. Hodgkins8, William J. Riley1, Virginia I. Rich8,9, Scott R. Saleska6, Matthew B. Sullivan8,9,10, Ruth K. Varner11, Gene W. Tyson12, Malak M. Tfaily13, Ahmed A. Zayed8,9<br> 1Climate and Ecosystem Sciences Division, Lawrence Berkeley National Laboratory; Berkeley, CA 94720, USA.<br>2Department of Environmental Science, Policy and Management, University of California; Berkeley, CA 94720, USA.<br>3Department of Biology, Case Western Reserve University; Cleveland, OH, USA, 44106<br>4Earth Ocean and Atmospheric Sciences, Florida State University; Tallahassee, FL, USA<br>5Department of Natural Resources and the Environment, University of New Hampshire;<br>Durham, NH, USA 03824<br>6Department of Ecology and Evolutionary Biology, University of Arizona; Tucson, AZ,<br>85721, USA<br>7Institut de Biologie de l’ENS, Université Paris Sciences & Lettres; Paris, 75005, France<br>8Department of Microbiology, The Ohio State University; Columbus, OH, USA, 43210<br>9Center of Microbiome Science, The Ohio State University; Columbus, Ohio 43210, USA.<br>10Department of Civil, Environmental and Geodetic engineering, The Ohio State University; Columbus, Ohio 43210, USA.<br>11Department of Earth Sciences and Institute for the Study of Earth, Oceans and Space, University of New Hampshire; Durham, NH 03824, USA.<br>12Centre for Microbiome Research, School of Biomedical Sciences, Queensland University<br>of Technology (QUT), Translational Research Institute; Woolloongabba, QLD, Australia<br>13Department of Environmental Science, University of Arizona; Tucson, AZ, 85721, USA</p>
ABRomics genomic paired-end FASTQ data demo files
<p>This dataset contains the demo files for the <em>Genomic paired-end FASTQ</em> template of the ABRomics platform:</p> <ul> <li>Raw data: Paired-end Illumina sequencing files of sample ARDIG49.</li> <li>Metadata: Filled out <em>Genomic paired-end FASTQ</em> template for sample ARDIG49.</li> </ul>
Data from: Genome assembly of Danaus chrysippus and comparison with the Monarch Danaus plexippus
<p><strong>GENOME ASSEMBLY DATA</strong></p> <p><strong>Dchry2_unaltered.fa.gz</strong><br> Unaltered version of Dchry2 before manual edits</p> <p><strong>Dchry2.haplotigs.fasta.gz</strong><br> Haplotypic contigs removed in the Purge_haplotigs step</p> <p><strong>Dchry2_to_Dchry2.2_transfers.txt</strong><br> Edits of the Dchry2 assembly to produce Dchry2.2 (contig breaks, reverse complements and name changes). Columns are: new contig name, new contig start, new contig end, original contig name, original contig start, original contig end, orientation. New contig numbers indicate the chromosome they correspond to.</p> <p><strong>mxv1.200520.ragoo.rnm.fa.gz</strong><br> Fasta file from of MEX_DaPlex assembly with mxdp_ fasta headers</p> <p><br> <strong>GENE AND REPEAT ANNOTATION</strong></p> <p><strong>danaus_plex_mex_braker_a002.sequences.tidy.gff3.gz</strong><br> Sorted and tidied gff3 from MEX_DaPlex re-annotation </p> <p><strong>danaus_plexv4_braker_a006.sequences.tidy.gff3.gz</strong><br> Sorted and tidied gff3 from Dplex_v4 re-annotation </p> <p><strong>mxv1.200520.ragoo.rnm.wDpv3.gff3.gz</strong><br> gff3 file from Cei of MEX_DaPlex annotation</p> <p><strong>dplex2_uniprot-proteome_UP000596680_and_dplex_mex.fasta.gz</strong><br> Protein set used for annotation of all three assemblies</p> <p><strong>functional_annotation_output.tar.gz</strong><br> all output from pannzer2 regarding functinal annotation of the Dchry2.2 genome</p> <p><strong>Lepidoptera_and_danaus_chrysippus2.2.repeatmasker.gz</strong><br> Custom repeat library (combination of lepidoptera and specific dchry2.2 library)</p>
Reconstruction of full-length LINE-1 progenitors from ancestral genomes (Supplementary Data)
<p><strong>Web Supplementary Files</strong></p> <ul> <li>Web Supplementary File 1 - FASTA files containing full-length reconstruction input sequences<strong>: full_length_reconstruction_input_sequence_fastas.zip</strong></li> <li>Web Supplementary File 2 - FASTA files containing Muscle alignments of the full-length reconstruction input sequences.<strong> full_length_reconstruction_input_sequence_alns.zip</strong></li> <li>Web Supplementary File 3 - FASTA file of full-length reconstructed sequences:<strong> full_length_reconstructions.fa</strong></li> <li>Web Supplementary File 4 - Table of full-length reconstruction statistics: <strong>full_length_reconstruction_stats.csv</strong></li> <li>Web Supplementary File 5 - FASTA files containing ORF reconstruction input sequences:<strong> orf_fastas.zip</strong></li> <li>Web Supplementary File 6 - FASTA files containing Macse alignments of the ORF reconstruction input sequences:<strong> ORF_reconstruction_input_sequence_alns.zip</strong></li> <li>Web Supplementary File 7 - Table of ORF reconstruction statistics: <strong>ORF_reconstructions.fa</strong></li> <li>Web Supplementary File 8 - Table of ORF reconstruction statistics: <strong>ORF_reconstruction_stats.csv</strong></li> <li>Web Supplementary File 9 - Table of Composite Sequences: <strong>bestfl_selection_fixed_CS_seqs.csv</strong></li> <li>Web Supplementary File 10 - Database of gold standards: <strong>L1_goldstandards.csv</strong></li> </ul> <p><strong>Data Underlying Figures</strong></p> <ul> <li>RepeatMasker scans of hg38 and ancestral genomes:<strong> </strong><strong>anc_gen_RM_out_files.zip</strong></li> <li><strong>Figure 4</strong> <ul> <li>4A <ul> <li>Source alignment of 54 composite sequences: <strong>220121_dropped12+L1ME3A_muscle.nt.afa</strong></li> <li>Tree produced using the alignment and FastTree: <strong>220121_dropped12+L1ME3A.tree</strong></li> </ul> </li> <li>4B <ul> <li>Source alignment of 67 Dfam L1 subfamily 3’ end models: <strong>200123_dfam_3ends.fa.muscle.aln</strong></li> <li>Tree produced using the alignment: <strong>200123_dfam_3ends.fa.muscle.aln.tree</strong></li> </ul> </li> </ul> </li> <li><strong>Figure 5</strong> <ul> <li>KZFP-TE enrichment p-values (from Barazandeh <em>et al</em> 2018):<strong> TE_KZFP_enrichment_pvals.xlsx</strong></li> <li>KZFP-TE top 500 peak overlap (from Barazandeh <em>et al</em> 2018): <strong>top500_peak_overlap.xlsx</strong></li> </ul> </li> <li><strong>Figure 6</strong> <ul> <li>RepeatMasker .out file for the Composite Sequence custom library queried against hg38: <strong>CS_RM_hg38.fa.out.gz</strong></li> </ul> </li> <li><strong>Figure S2</strong> <ul> <li>RepeatMasker scan .out file of hg38 (CG corrected Kimura Divergence values are in last column): <strong>hg38+KimDiv_RM.out</strong></li> <li>RepeatMasker scan .out file of the Progressive Cactus eutherian ancestral genome (CG corrected Kimura Divergence values are in last column): <strong>Progressive_Cactus_Euth+KimDiv_RM.out</strong></li> <li>RepeatMasker scan .out file of the Ancestors 1.1 eutherian ancestral genome (CG corrected Kimura Divergence values are in last column): <strong>Ancestors_Euth+KimDiv_RM.out</strong></li> </ul> </li> <li><strong>Figure S5</strong> <ul> <li>RepeatMasker scan .out files for Progressive Cactus simian and primate reconstructed ancestral genomes: <strong>progCactus_RM_outfiles.zip</strong></li> <li>S5A <ul> <li>FASTA files containing Cactus genome-derived reconstructed sequences equivalent to the L1MA2, L1MA4, and L1MD1-3 best full-length sequences: <strong>progCactus_reconstruction_bestFL_equivalents.zip</strong></li> </ul> </li> <li>S5B <ul> <li>FASTA files containing Muscle alignments of Cactus genome-derived full-length reconstruction input sequences: <strong>progCactus_reconstruction_input_sequence_alns.zip</strong></li> </ul> </li> </ul> </li> <li><strong>Figure S6</strong> <ul> <li>S6A <ul> <li>Results of Conserved Domain scans of Cactus genome-derived full-length reconstructed sequences: <strong>CD_search_results_short_nms.txt</strong></li> </ul> </li> <li>S6B-D <ul> <li>Character posterior probabilities of “best” full-length reconstructed sequences: <strong>best_fl_post_probs.zip</strong></li> </ul> </li> </ul> </li> <li><strong>Figure S7</strong> <ul> <li>S7B-C <ul> <li>Results of Conserved Domain scans of translated initial full-length reconstructed sequences: <strong>initial_recons_all_3frametrans_CD-search.txt</strong></li> <li>Results of Conserved Domain scans of translated reconstructed ORFs: <strong>recons_ORF1-2_all_3frametrans_CD-search.csv</strong></li> </ul> </li> </ul> </li> <li><strong>Figure S15</strong> <ul> <li>S15A <ul> <li>Source alignment of 67 composite sequences: <strong>bestfl_selection_fixed_CS_seqs_muscle.nt.afa</strong></li> <li>Tree produced using the alignment: <strong>bestfl_selection_fixed_CS_seqs_muscle.nt.afa.tree</strong></li> </ul> </li> <li>S15B-E <ul> <li>Source Muscle alignments for phylogenetic trees of reconstructed sequence components: <ul> <li>ORF2: <strong>ORF2_keep54_muscle.nt.afa</strong></li> <li>5’ UTR: <strong>5utr_keep54_muscle.nt.afa</strong></li> <li>ORF1: <strong>ORF1_keep54_muscle.nt.afa</strong></li> <li>3’ UTR: <strong>3utr_keep54_muscle.nt.afa</strong></li> </ul> </li> <li>Trees produced using above alignments: <ul> <li>ORF2: <strong>ORF2_keep54_muscle.nt.afa.tree</strong></li> <li>5’ UTR: <strong>5utr_keep54_muscle.nt.afa.tree</strong></li> <li>ORF1: <strong>ORF1_keep54_muscle.nt.afa.tree</strong></li> <li>3’ UTR: <strong>3utr_keep54_muscle.nt.afa.tree</strong></li> </ul> </li> </ul> </li> </ul> </li> <li><strong>Figure S17</strong> <ul> <li>Unfiltered BLAST results of Composite Sequences queried against hg38: <strong>CS_hg38_blastn.csv.zip</strong></li> <li>BED file of L1 instances annotated using BLAST pipeline: <strong>BLAST_L1_hits.bed</strong></li> </ul> </li> </ul>
Whole-genome genotype data for French Large White pigs from two distinct sampling times
<p>Genotype data at plink binary format for 36 pigs from the french Large White breed: 13 animals from the female line born in 2014 and 2015, 13 animals from the male line born between 2012 and 2016, and 10 animals from a common ancestral line, born in 1977. These genotypes were obtained from individual whole genome sequencing (WGS) data, whiwh are available at https://www.ebi.ac.uk/ena under the accession number PRJEB51909.</p> <p>Two different genotype datasets were obtained from the raw WGS:</p> <p>1) snp20_auto_cr (.bed/bim/fam): High quality autosomal SNPs, called by 3 different software, with a call rate of at least 90%</p> <p>2) all10_auto (.bed/bim/fam): All SNPs or indels called by at least one of 3 different software.</p> <p>More details about these datasets and their use can be found in the following study:</p> <p>Boitard et al (under revision): Whole-genome sequencing of cryo-preserved resources from French Large White pigs at two distinct sampling times reveals strong signatures of convergent and divergent selection between the dam and sire lines.</p>
Endosymbiont mining from Lepidoptera genomics data
<p>Alignments and assembly files from the manuscript: 'One’s trash is someone else’s treasure: Sequence read archives from Lepidoptera genomes provide material for genome reconstruction of their endosymbionts'</p>
Data and code for the publication "DNA methylation underpins the epigenomic landscape regulating genome transcription in Arabidopsis"
<p>The zipped file of this repository contains code and data to reproduce the results of the publication:</p> <p>Zhao et al, DNA methylation underpins the epigenomic landscape regulating genome transcription in Arabidopsis. Genome Biology (2022). </p> <p>All sequence data have been deposited in NCBI GEO accession codes GSE183987 and GSE169497.</p> <p> </p> <p>Please see the README document for detailed:</p> <p>- Descriptions of the code and data provided</p> <p>- Lists of the required dependencies</p>
Training data for 'Preparing genomic data for phylogeny reconstruction' (Galaxy Training Material)
<p>This data is used for Galaxy Training Network training 'Preparing genomic data for phylogeny reconstruction'. There are four nucleotide sequences from chromosome 5 of four strains of S. cerevisiae. The GenBank annotated sequenced were produced using 'funannotate predict annotation' (Galaxy Version 1.8.9+galaxy2) on the nucleotide sequences sequences. References: DOI: 10.1126/science.274.5287.546; DOI: 10.1126/science.1189015; DOI: 10.1016/j.cell.2016.08.020</p>
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Allen Brain Atlas
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Annotated Behaviour and Observability Dataset (ABODe)
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