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3 results for “Inner filter effect”
Inner filter effect correction for fluorescence measurements in microplates (ZINFE and NINFE) - experimental data
<p>Experimental data for the paper entitled <em>Inner Filter Effect Correction for Fluorescence Measurements in Microplates Using Variable Vertical Axis Focus</em> (https://doi.org/10.1021/acs.analchem.2c01031).</p> <p>Separate datasets are provided for data with background correction, without background correction, and absorbance-corrected data.</p> <p>All results were obtained using the online calculator service written in Javascript: https://ninfe.science (version 15.9.2021.).</p> <p>For additional details please visit: https://glymech.pharma.hr//GlyMech.html.</p> <p> </p>
Inner filter effect correction for fluorescence measurements in microplates (AddAbs) - experimental data
<p>Experimental data for the paper entitled <em>Reducing the Inner Filter Effect in Microplates by Increasing Absorbance? Linear Fluorescence in Highly Concentrated Fluorophore Solutions in the Presence of an Added Absorber</em> (<a href="https://doi.org/10.1021/acs.analchem.3c01295">https://doi.org/10.1021/acs.analchem.3c01295</a>).</p> <p>Separate worksheets are provided for the following:</p> <p>1. Fluorescence measurements - raw values in triplicate; 2 worksheets for transparent (T) and nontransparent (NT) microplates, each with 15 titrations: L<sub>1</sub>-L<sub>12</sub> and H<sub>1</sub>-H<sub>3</sub>,</p> <p>2. Absorbance measurements - raw values in triplicate (<em>λ</em><sub>ex</sub> = 345 nm, <em>λ</em><sub>em</sub> = 390 nm); 1 worksheet for T microplates only, with 4 titrations: L<sub>1</sub>-L<sub>4</sub>,</p> <p>3. Fluorescence measurements - averaged, baseline-corrected averaged and normalized baseline-corrected averaged triplicates; 2 worksheets for T and NT microplates, each with 15 titrations: L<sub>1</sub>-L<sub>12</sub> and H<sub>1</sub>-H<sub>3</sub>,</p> <p>4. ZINFE/NINFE-corrected fluorescence in T microplates; 12 worksheets for 12 titrations: L<sub>1</sub>-L<sub>9</sub> and H<sub>1</sub>-H<sub>3</sub>,</p> <p>5. ZINFE/NINFE-corrected fluorescence in NT microplates; 12 worksheets for 12 titrations: L<sub>1</sub>-L<sub>9</sub> and H<sub>1</sub>-H<sub>3</sub>.</p> <p>The results of the ZINFE and NINFE correction methods obtained using the online calculator service written in Javascript: https://ninfe.science (version 09.5.2022.)</p> <p>For additional details please visit: https://glymech.pharma.hr//GlyMech.html.</p>
Chromophore-driven optical path reduction as an indirect inner filter effect correction strategy in fluorescence microplate assays - experimental data
<p>Experimental data for the paper entitled <em>Chromophore-driven optical path reduction as an indirect inner filter effect mitigation strategy in fluorescence microplate assays</em> (<a title="Persistent link using digital object identifier" href="https://doi.org/10.1016/j.saa.2025.127049" target="_blank" rel="noreferrer noopener"><span><span>https://doi.org/10.1016/j.saa.2025.127049</span></span></a>). </p> <p>An Excel file with multiple worksheets is provided for the following:</p> <p>1. Fluorescence data:</p> <p>The worksheets contain fluorescence values measured in tetraplicates using two microplate readers (Tecan Spark M10, Tecan, Austria and SpectraMax iD3, Molecular Devices, USA), with baseline values recorded in octuplicates. The results were averaged and baseline-corrected. Detailed notes on the titration experiments are included in the manuscript. Data include both IFE-uncorrected fluorescence and fluorescence corrected (fully or partially) by the addition of an absorbing chromophore.</p> <p>2. Absorbance data:</p> <p>All measured absorbance values obtained using the Tecan Spark M10 microplate reader with UV-Vis transparent microplates are included. The absorbance values at specific wavelengths suitable for the Lakowicz IFE correction method or for determining the concentration of the compound are given separately.</p> <p>3. Fluorescein spectra:</p> <p>UV-Vis and fluorescence excitation/emission spectra for highly dilute fluorescein concentrations where the inner filter effect (IFE) is negligible are reported. These measurements were performed to obtain “true” excitation and emission spectra without the influence of IFE quenching effects.</p> <p>4. ZINFE/NINFE IFE corrections:</p> <p>The dataset contains the results of ZINFE/NINFE IFE corrections (<a href="https://doi.org/10.1021/acs.analchem.2c01031">https://doi.org/10.1021/acs.analchem.2c01031</a>) applied to the fluorescence data and processed using the web service available at <a href="https://ninfe.science">https://ninfe.science</a>.</p> <p>The notation of the fluorophores and microplates is the same as in the manuscript. For reasons of visual clarity, the numerical titration indices are not in subscript.</p> <p> </p>
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