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16
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ShareScore release 0.9.0
Dataset results
16 results for “Insect cell sf9”
Small scale test-expression of full-length huntingtin Q23 with HAP40 in baculoviral expression system production in sf9 insect cells – 2018/05/14
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Small scale test-expression of full-length huntingtin Q23 with HAP40 in baculoviral expression system production in sf9 insect cells – 2018/05/14. To validate our insect cell production system for the generation of physiologically relevant huntingtin constructs, HTT and HAP40 will be co-expressed and purified from sf9 culture. </p>
Large scale expression and purification of full-length huntingtin Q23 with and without HAP40 from baculoviral expression system production in sf9 insect cells – 2018/06/04
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples with and without stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/05/28
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples with stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
Large scale expression and purification of full-length huntingtin Q19 and Q23 from baculoviral expression system production in sf9 insect cells – 2018/06/18
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Large scale expression and purification of full-length huntingtin Q23 and Q54 from baculoviral expression system production in sf9 insect cells – 2018/07/06
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Attempted expression and purification of full-length huntingtin Q23 with putative interaction partners from baculoviral expression system production in sf9 insect cells – 2018/07/19
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale: </strong>To obtain monodisperse and conformationally constrained huntingtin protein samples for high resolution structural biology, interaction partners are required as highlighted by <a href="https://www.nature.com/articles/nature25502">Guo et al (2018)</a>.</p>
Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/07/11
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Large scale expression and purification of full-length huntingtin Q42 from baculoviral expression system production in sf9 insect cells – 2018/07/10
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Experiment and rationale</strong> - Purified huntingtin samples are required for use in structural and functional studies. </p>
Large scale expression and purification of huntingtin fragment A667-C3140 from baculoviral expression system production in sf9 insect cells – 2018/08/02
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale: </strong>To obtain monodisperse and conformationally constrained huntingtin protein samples for high resolution structural biology.</p>
Expression and purification of HTT79-3144 with and without HAP40 from Sf9 Insect cells - 2019/01/28
<p><strong>Project: </strong>Biophysical investigation of purified HTT protein samples</p> <p><strong>Experiment: </strong>Expression and purification of HTT<sup>79-3144 </sup>with and without HAP40 from Sf9 Insect cells</p> <p><strong>Date completed:­ </strong>2019/01/28</p> <p><strong>Rationale: </strong>HTT lacking the unstructured region of exon1 (aa. 1-78 which are not resolved in the cryoEM model PDB ID 6EZ8) may serve as a useful control when determining exon1-dependent functionality of the HTT protein. Apo and HAP40-bound HTT<sup>79-3144 </sup>will be produced from Sf9 insect cell expression system. </p>
Multi-angle light scattering (MALS) analysis of HTT1-3144 Q54 HAP401-371 samples purified from Sf9 insect cells - 2018/12/03
<p><strong>Project: </strong>Biophysical investigation of purified HTT protein samples</p> <p><strong>Experiment: </strong>Multi-angle light scattering (MALS) analysis of HTT<sup>1-3144 </sup>Q54 HAP40<sup>1-371</sup>samples purified from Sf9 insect cells</p> <p><strong>Date completed:­ </strong>2018/12/03</p> <p><strong>Rationale: </strong>Apo HTT<sup>1-3144 </sup>Q23 and HTT<sup>1-3144 </sup>Q23 HAP40<sup>1-371</sup>complex samples have been analysed by MALS revealing HTT-HAP40 complex samples to be monodisperse. PolyQ expanded HTT constructs have been expressed as HAP40 complexes but the monodispersity has not been assessed beyond analytical gel filtration. </p>
Purification of full-length huntingtin (HTT) constructs Q23 and Q46 from insect sf9 cells and preparation of samples for electron microscopy (2017/03/30)
<p>Huntingtin structure-function open lab notebook project</p>
Expression and purification of PARP1 from Sf9 Insect cells 2019/02/04
<p><strong>Project: </strong>Investigating HTT parylation and PAR-binding in its putative role in DNA damage repair</p> <p><strong>Experiment: </strong>Expression and purification of PARP1 from Sf9 Insect cells</p> <p><strong>Date completed:­ </strong>2019/02/04</p> <p><strong>Rationale: </strong>HTT may be parylated or recognize PAR motifs on other proteins involved in DNA damage repair. To test this hypothesis, the full-length PARP1 cDNA was cloned in to pFBOH-SBP-TEV-LIC, overexpressed in insect cell Sf9 culture and purified. The purified PARP1 protein will be shared with Dr. Tamara Maiuri for her future experiments on the link between HTT protein and parylation. </p>
Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/08/04
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale </strong>- Purified huntingtin samples with and without stabilising binding partners (HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
Characterization of Residual MicroRNAs in AAV Vector Batches Produced in HEK293 Mammalian Cells and Sf9 Insect Cells
GEO Series GSE270691. Homo sapiens; unclassified Adeno-associated dependoparvovirus A. 3 samples. Type: Expression profiling by RT-PCR.
Effect of 11 xenobiotics on Sf9 cells and insect midgut
GEO Series GSE32486. Spodoptera frugiperda. 132 samples. Type: Expression profiling by array.
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