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opencc-by-4.0Jun 2022View details →

Reims Trematodes MSP Database V1

MALDI TOF Bruker MSP database for Trematoda. Described in "MALDI-TOF : A new tool for the identification of Schistosoma cercariae and detection of hybrids" (publication pending) and  Huguenin, Antoine, et al. 2019. « MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea) ». Parasite 26 (8): 11‑13. <a href="https://doi.org/10.1051/parasite/2019011">https://doi.org/10.1051/parasite/2019011</a>.

opencc-zeroJun 2024View details →

Dataset: MSP Recovery, Inc. (LIFWW) Stock Performance

This dataset provides historical stock market performance data for specific companies. It enables users to analyze and understand the past trends and fluctuations in stock prices over time. This information can be utilized for various purposes such as investment analysis, financial research, and market trend forecasting.

opencc-zeroJun 2024View details →

Dataset: MSP Recovery, Inc. (LIFW) Stock Performance

Fig. 4 in Lungworm seroprevalence in free-ranging harbour seals and molecular characterisation of marine mammal MSP

Fig. 4. Phylogenetic tree from maximum likelihood analysis of MSP nucleotide sequences of marine and terrestrial mammal parasitic nematodes including GenBank accession numbers. The percentage of replicate trees in which the associated species clustered together in the bootstrap test (1000 replicates) is shown next to the branches.

Fig. 5 in Lungworm seroprevalence in free-ranging harbour seals and molecular characterisation of marine mammal MSP

Fig. 5. Phylogenetic tree from maximum likelihood analysis of MSP amino acid sequences of marine and terrestrial mammal parasitic nematodes including GenBank accession numbers. The percentage of replicate trees in which the associated species clustered together in the bootstrap test (1000 replicates) is shown next to the branches. *amino acid sequence translated from EST sequences.

Fig. 3 in Lungworm seroprevalence in free-ranging harbour seals and molecular characterisation of marine mammal MSP

Fig. 3. Alignment of MSP amino acid sequences of marine and terrestrial mammal parasitic nematodes using the Clustal W method.

Fig. 2 in Lungworm seroprevalence in free-ranging harbour seals and molecular characterisation of marine mammal MSP

Fig. 2. Alignment of MSP nucleotide sequences of marine and terrestrial mammal parasitic nematodes using the Clustal W method.

Fig. 1 in Lungworm seroprevalence in free-ranging harbour seals and molecular characterisation of marine mammal MSP

Fig. 1. Anthelmintic treatment pattern, blood sampling time points and corresponding OD values of harbour seals in rehabilitation. Harbour seals (n = 6) were treated twice with ivermectin at arrival (day 1) at the Seal Rehabilitation and Research Centre, Pieterburen, The Netherlands, and 21 days later and once with mebendazole between day 2 and 6. OD values in grey boxes show lungworm-ELISA positive serum samples, those highlighted in blue lungworm-ELISA negative samples. Harbour seal individuals highlighted in dark grey coughed up lungworms one day after arrival. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Fig. 6 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)

Fig. 6. Analysis of the sensitivity and specificity of the P. relictum MSP-1 capture antigen-based ELISA. Serial dilutions of normal chicken serum and three sera samples from P. relictum-infected penguins (8776, 8783 and 8784) were used for coating the assay wells. Each dilution was performed in triplicate, and the data shown represent means of three independent experiments with standard error bars and levels of statistical significance (****: P <0.0001).

Fig. 4 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)

Fig. 4. Validation of ELISA. The ELISA developed using P. relictum MSP-1 protein was tested with known P. relictum positive penguin serum collected from penguin # 8790 at week 26. Black column represents positive penguin serum. Hatched and white columns represent negative controls containing normal chicken serum and PBS, respectively. Grey column represents the positive reaction containing streptavidin alkaline-phosphatase and its substrate pnitrophenyl phosphate. Each reaction was performed in triplicate, and the data shown represent means of three independent experiments with standard error bars and levels of statistical significance (****: P <0.0001).

Fig. 2 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)

Fig. 2. Verification of the biotinylation of recombinant P. relictum MSP-1. Purified recombinant P. relictum MSP-1 protein was labeled with biotin. Biotinylation of MSP-1 was confirmed using an enzyme (streptavidin alkaline phosphatase)-linking assay and absorbance read at 405 nm indicated the presence of biotinylated MSP-1. Black column represents biotinylated MSP-1 protein. Hatched column and white column represent negative controls containing non-biotinylated MSP-1 and PBS, respectively. Grey column represents the positive control containing streptavidin alkaline-phosphatase (SAP) and its substrate p-nitrophenyl phosphate (PNPP). Each reaction was performed in triplicate, and the data shown represent means of three independent experiments with standard error bars and levels of statistical significance (****: P <0.0001).

Fig. 3 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)

Fig. 3. Biotin-labeled MSP-1 protein titration curve. Serially diluted (300 ng/ μL to 0.003 ng/μL) biotinylated MSP-1 was used for coating the surfaces of the reaction wells overnight at 4 ◦C. The amount of biotinylated MSP-1 immobilized on the surface of the well was proportional to the intensity of the colored product generated which in turn was proportional to the absorbance value measured at 405 nm wavelength. Reactions were performed in triplicate, and the data shown represent means of three independent experiments with standard error bars.

Fig. 7. MSP-1 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)

Fig. 7. MSP-1 capture antigen-based ELISA analysis of 370 sera samples collected from 11 penguins during three consecutive seasons of Spring (March to May), Summer (June to August) and Fall (September to November). (A) ELISA absorbance readings for the 370 sera samples collected from Spring to Fall are arranged in ascending order. Time of collection is indicated by color. Light grey represents Spring, dark grey represents Summer, and black represents Fall. Black dashed line indicates the single cut-off point (0.488) determined by change-point analysis. (B) Left: percentage of positive (A405 ≥ 0.488) and negative (A405 <0.488) sera samples; Right: distribution of positive samples in Spring (March to May), Summer (June to August), and Fall (September to November). (C) Percentage of penguin sera samples that tested positive (A405> = 0.488) in each month from March to November.

Fig. 5. P. relictum MSP-1 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)

Fig. 5. P. relictum MSP-1 capture antigen-based ELISA analysis of test sera from penguins. Sera samples (370 total) from eleven penguins collected from Spring through Fall season were used as test samples in the assay to determine anti-P. relictum antibodies level. Light grey, dark grey, and black columns represent penguin sera samples collected in Spring, Summer, and Fall, respectively. Panel A–K represents ELISA results for individual penguins' sera collected at different time points from Spring to Fall. Panel L represents average ELISA absorbances for all 11 penguins at different sampling points. Each sample was assayed in triplicate, and the data shown represent means of three independent experiments with standard error bars.