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Fig. 1 in 17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxyprogesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)

Fig. 1. Radiochromatogram after TLC analysis of spermiating testes of Micropogonias furnieri males 1, 2 and 3 after toluene/cyclohexane 50/50 v/v (system I) and benzene/acetone 80/20 v/v (system II). The TLC zones further analyzed by HPLC are shown as A, B and C. The peak of precursor is shown as D. The authentic standards used were: 1 = 20-dihydrocortisone; 2 = 17,20,21-P; 3 = 17,20α-P; 4 = 11KT; 5 = 17,20ss-P; 6 = 11ssOH-A; 7 = Ad; 8 = testosterone; 9 = 17-P; 10 = A.

Figures 15-20 in The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study

Figures 15-20. Frequency histogram of fiber diameters of the adductor fin muscle of Micropogonias furnieri. (15). Red and pink fibers. (16). Red and white fibers from the proximal zone. (17). Red fibers and white fibers from the distal zone. (18). Pink and white fibers from the proximal zone. (19). Pink and white fibers from the distal zone. (20). White fibers from the proximal and distal zones. Pink line, red fibers; blue line, pink fibers; purple line, proximal zone white fibers; green line, distal zone white fibers.

Figures 12-14 in The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study

Figures 12-14. Histochemical staining of mATPase for capillaries from the adductor fin muscle of Micropogonias furnieri. (12). The number of capillaries (black arrow) in red fibers (r) is greater than those found in the other fiber types; pink fibers (p) have an intermediate irrigation, (bar = 150 µm). (13). The technique shows two different white fibers zones: proximal (wprox) and distal (wdis), (bar = 50 µm). (14). The white fibers proximal (wprox) zone exhibits more irrigation than the distal zone (black arrow: capillaries), (bar = 130 µm).

Figures 8-11 in The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study

Figures 8-11. Histochemical staining of mATPase activity in the adductor fin muscle of Micropogonias furnieri. (8). mATPase activity with pre-incubation at pH 10.4 showing red and pink fibers; small and medium fibers lose their activity; large fibers show a strong reaction (lp: large pink, lr: large red, mp: medium pink, mr: medium red, sp: small pink, sr: small red), (bar = 100 µm). (9). mATPase activity with pre-incubation at pH 10.4. A homogeneous staining of white fibers of the proximal zone is shown (lp: large pink, lwp: large white proximal, mp: medium pink, mwp: medium white proximal, sp: small pink, swp: small white proximal), (bar = 120 µm). (10). mATPase activity with pre-incubation at pH 4.3; white fibers of the proximal zone showing a mosaic pattern of staining (lwp: large white proximal, mwp: medium white proximal, p: pink, swp: small white proximal), (bar = 120 µm). (11). mATPase with pre-incubation at pH 4.3 showing white fibers of distal zone; small white fibers (swd) exhibit a strong reaction; medium (mwd) and large (lwd) fibers show a moderate reaction, (bar = 80 µm).

Figures 4-7 in The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study

Figures 4-7. Histochemical staining of red, pink and white fibers from the adductor fin muscle of Micropogonias furnieri. (4). Red fibers showing strong staining (SDH technique); pink fibers showing weak reaction to SDH (lp: large pink, lr: large red, mp: medium pink, mr: medium red, sp: small pink, sr: small red), (bar = 100 µm). (5). Periodic acid-Schiff (PAS) showing small red fibers (sr) with very strong stain intensity, and small pink (sp) fibers with moderate activity; medium and large fibers react weakly to PAS (lp: large pink, lr: large red, mp: medium pink, mr: medium red), (bar = 50 µm). (6). The small (swp) and medium (mwp) white fibers of the proximal zone show a weak reaction to SDH; large fibers (lwp) have no reaction, (bar = 100 µm). (7). White fibers of the proximal zone react weakly to PAS; white fibers of the distal zone have no reaction (lwd: large white distal, lwp: large white proximal, mwd: medium white distal, mwp: medium white proximal, swd: small white distal, swp: small white proximal), (bar = 150 µm).

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Figure 5. - Otolith shape variation associated with A in Otolith shape differences between Micropogonias manni and M. altipinnis (Sciaenidae)

Figure 5. - Otolith shape variation associated with A: body-weight; B: standard length; C: total length of specimens. Grey lines correspond to low BW and SL/TL; black lines correspond to high BW and SL/TL.

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Figure 1 in Otolith shape differences between Micropogonias manni and M. altipinnis (Sciaenidae)

Figure 1. - Current distribution range of Micropogonias altipinnis (light grey) and Micropogonias manni (dark gray).

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Figure 2 in Otolith shape differences between Micropogonias manni and M. altipinnis (Sciaenidae)

Figure 2. - Left sagitta of Micropogonias altipinnis (A) and M. manni (B); mesial view. Scale bar: 5 mm.

Fig. 3 in 17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxyprogesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)

Fig. 3. Analysis of zone C of TLC or di-hydroxylated progestins area (see Fig. 1) by HPLC using methanol/acetonitrile/water 33/26/41 v/v/v (system III) in Micropogonias furnieri.

Fig. 4 in 17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxyprogesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)

Fig. 4. Plasma level of 17,20ss-P in males at different stages of development sampled at the Río de la Plata estuary. 1: Immaturetestis; 2: Developingtestis (earlyspermatogenesis); 3: Fully developed testis (whole spermatogenesis), 4: Spermiating testis; 6: Resting testis. n = number of fish collected for each stage.

Fig. 2 in 17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxyprogesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)

Fig. 2. Analysis of zone B of TLC or cortisol area (see Fig. 1) by HPLC using methanol/acetonitrile/water 33/26/41 v/v/v (system III) in Micropogonias furnieri.

Figures 1-3 in The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study

Figures 1-3. Diagram of the adductor pectoral fin muscles of Micropogonias furnieri. (1). Superficial view showing the adductor superficialis (I), adductor medialis (II) and adductor radialis (III), (bar = 1cm). (2). Superficial view showing adductor medialis (II), adductor radialis (III), adductor profundus (V) and arrector dorsalis (IV); no adductor superficialis is evidenced, (bar = 1cm). (3). Superficial view showing adductor profundus (V) and arrector dorsalis (IV); no adductor medialis is evidenced, (bar = 1cm).