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20 results for “Peptide screening”
A Multiplexed Cell-Free Assay to Screen for Antimicrobial Peptides in Double Emulsion Droplets
<p>Data underlying the figures in the publication “A Multiplexed Cell-Free Assay to Screen for Antimicrobial Peptides in Double Emulsion Droplets”, published in <em>Angew. </em><em>Chem. Int. Ed.,</em> <strong>2022</strong>, e202114632.</p> <p><a href="https://onlinelibrary.wiley.com/doi/10.1002/anie.202114632">https://onlinelibrary.wiley.com/doi/10.1002/anie.202114632</a></p> <p> </p> <p>Table of contents:</p> <p><strong>1. Figure 1b</strong>: Bright-field image of the double emulsions droplets produced on the microfluidic chip (scale bar 40 μm).</p> <p><strong>2. Figure 1c</strong>: Source video of the image in <em>Figure 1c</em>. Overlaid fluorescence and bright-field image of a double emulsion in a hydrodynamic trap, containing LUVs loaded with a self-quenching concentration of SRB in the cell-free extract, showing background fluorescence (scale bar 20 μm).</p> <p><strong>3. Figure 2a</strong>: Excel file containing the experimental data for <em>Figure 2a</em>. Cell-free protein production. Cell-free production of sfGFP in double emulsion (DE) droplets. The expression and folding of sfGFP was confirmed by the increase of fluorescence at 516 nm (ex. 488 nm). The dashed ribbon represents standard deviation (n=150).</p> <p><strong>4. Figure 2c</strong>: Excel files containing the experimental data for <em>Figure 2c</em>. Mean fluorescence intensities of b) after incubation at room temperature for 16 hours. no DNA: DEs without any alpha-hemolys in plasmid DNA(n=107), α-HL:DEs with the alpha-hemolys in plasmid DNA(n=258), SDS: double emulsions without any alpha-hemolys in plasmid DNA, exposed to a solution of 0.5% SDS in buffer throughout the incubation (n=204).</p> <p><strong>5. Figure 2d</strong>: Excel file containing the experimental data for <em>Figure 2d</em>. Fluorophore leakage kinetics from mammalian-like LUVs with SRB and from bacteria-like LUVs with 6-FAM, induced by the cell-free expression of pneumolysin in a 384 well-plate, starting at time 0. Fractional fluorescence (fF) is calculated by setting the zero level to the vesicle fluorescence in the absence of DNA, and the maximum level of fluorescence, scaled to a value of 1, to the value obtained by lysing the vesicles with 0.5% SDS. Solid lines represent the average of three independent reactions visible below.</p> <p><strong>6. Figures 2e and 2f</strong>: FACS data for <em>Figures 2e</em> and <em>2f</em>.</p> <p><strong>7. Figure 3a</strong>: Excel file containing the experimental data for <em>Figure 3a</em>. Fluorophore leakage kinetics from mammalian-like LUVs with SRB and bacteria-like LUVs with 6-FAM, induced by the cell-free expression of meucin-25 in a 384 well-plate. Each well contained 8 nM of plasmid (Supporting Information Table 1). Solid lines represent the average of three technical replicates displayed as well (the lines are overlapping, thus not visible).</p> <p><strong>8. Figure 3c</strong>: Excel file containing the experimental data for <em>Figure 3c</em>. Bacterial viability assay with increasing meucin-25 concentrations, measured by flow cytometry. Propidium iodide (PI) cannot pass intact bacterial membranes and only intercalates the DNA of permeabilized dead bacteria (“PI positive”). Constitutively expressed sfGFP proteins normally efficiently retained in intact bacterial cells (“GFPpositive”) but lost in suitably permeabilized cells. Error bars indicate standard deviation (n=10000).</p> <p><strong>9. Figure SI_2</strong>: Excel files containing the experimental data for <em>Supplementary Figure 2</em>.</p> <p><strong>10. Figure SI_3</strong>: Excel file containing the experimental data for <em>Supplementary Figure 3</em>.</p> <p><strong>11. Figure SI_4a</strong>: Excel files containing the experimental data for <em>Supplementary Figure 4a</em>.</p> <p><strong>12. Figure SI_4b</strong>: Excel files containing the experimental data for <em>Supplementary Figure 4b</em>.</p> <p><strong>13. Figure SI_5</strong>: Excel files containing the experimental data for <em>Supplementary Figure 5</em>.</p> <p><strong>14. Figure SI_6</strong>: Excel files containing the experimental data for <em>Supplementary Figure 6</em>.</p> <p> </p> <p> </p>
Screen of A6 TCR against a library of HLA-A*02:01 MHC-I peptides from the human exome
<p>T2 cells expressing a library of off targets (derived from A6 and B7 binding motifs in Hausmann 1999) are co-cultured with A6, DMF5 or 1G4 expressing T cells (from a non-A2 donor) and minigenes from surviving cells are amplified.</p>
Screen of Pr20 TCR mimic antibody against a library of HLA-A*02:01 MHC-I peptides
<p>Minigene sequencing of T2 cells sorted for high and low binding to the TCR mimic antibody "Pr20."</p>
Screen of A6 and B7 TCR against a library of HLA-A*02:01 MHC-I peptides from the human exome
<p>T2 cells expressing a library of off targets (derived from A6 and B7 binding motifs in Hausmann 1999) are co-cultured with A6, B7. DMF5 or 1G4 expressing T cells (from a non-A2 donor) and minigenes from surviving cells are amplified.</p>
Screen of A6,B7,1G4 TCRs against a library of HLA-A*02:01 MHC-I peptides from the human exome
<p>T2 cells expressing a library of off targets (derived from A6 and B7 binding motifs in Hausmann 1999) are co-cultured with A6, B7 or1G4 expressing T cells (from a non-A2 donor). Minigenes from surviving cells are amplified and sequenced.</p>
Screen of ESK1 TCR mimic antibody against a library of HLA-A*02:01 MHC-I peptides
<p>Minigene sequencing of T2 cells sorted for high and low binding to the TCR mimic antibody "ESK1."</p>
Data for: Screening of stapled peptides for inhibition of calcium-triggered exocytosis
Open the record for dataset details and reuse information.
Screening and mechanism of novel angiotensin-I-converting enzyme inhibitory peptides in X. sorbifolium seed meal: A computer-assisted experimental study method
<p>本文档是补充材料</p>
Screening for novel carbon nanotubes binding-peptides
<p>This is the supplementary material section of the article "<strong>Screening for novel carbon nanotubes binding-peptides</strong>".</p> <p>Contents: Table S1-S9, contains all discovered peptides and intensities generated from the nanoLC-MS-MS experiment.</p>
Next-Generation Antimicrobial Discovery through Bacterial Self-Screening of Surface-Displayed Peptide Libraries [HiSeq]
GEO Series GSE94529. Escherichia coli str. K-12 substr. W3110. 4 samples. Type: Other.
High-throughput screening for cell binding and repulsion peptides on multifunctionalized surfaces
<p><a name="_GoBack"></a>Tables with fluorescent intensity values after cell incubation for each peptide from libraries synthesized on peptide chips. Excel file “Supplementary Data 1.xlsx” represents the numerical source data for graphs Fig 2, Fig3, suppl Fig 1, suppl Fig 2. Excel file “Supplementary Data 2.xlsx” represents the numerical source data for suppl Fig. 3.</p>
A Prospective Study to Assess the Screening Value of N-terminal Pro-B-type Natriuretic Peptide (NT-proBNP) for the Identification of Patients That Benefit From Additional Cardiac Testing Prior to Vasc
ClinicalTrials.gov study NCT00519961. IPD Sharing: Not stated. Countries: 2. Publications: 0.
Screening for earlY Heart Failure Diagnosis and Management in Primary Care or at HOme Using Natriuretic Peptides and echocardiographY "SYMPHONY-HF"
ClinicalTrials.gov study NCT05919342. IPD Sharing: UNDECIDED. Countries: 5. Publications: 0.
ER alpha coregulator peptide screening profiling by MARCoNI (Microarray Assay for Real-time Coregulator-Nuclear receptor Interaction) assay from human embryonic kidney HEK293 cells after tranfected wi
GEO Series GSE155085. Homo sapiens. 42 samples. Type: Protein profiling by protein array.
Next-Generation Antimicrobial Discovery through Bacterial Self-Screening of Surface-Displayed Peptide Libraries
GEO Series GSE94531. Escherichia coli str. K-12 substr. W3110. 12 samples. Type: Other.
Identification of endogenous small peptides involved in rice immunity through transcriptomics- and proteomics-based screening
GEO Series GSE252768. Oryza sativa Japonica Group. 21 samples. Type: Expression profiling by high throughput sequencing.
Next-Generation Antimicrobial Discovery through Bacterial Self-Screening of Surface-Displayed Peptide Libraries [MiSeq]
GEO Series GSE94530. Escherichia coli str. K-12 substr. W3110. 8 samples. Type: Other.
Identification of endogenous small peptides involved in rice immunity through transcriptomics- and proteomics-based screening
GEO Series GSE252769. Oryza sativa Japonica Group. 48 samples. Type: Expression profiling by high throughput sequencing.
Identification of endogenous small peptides involved in rice immunity through transcriptomics- and proteomics-based screening
GEO Series GSE252767. Oryza sativa Japonica Group. 27 samples. Type: Expression profiling by high throughput sequencing.
Raman-derived identification tags for mutation screening in amyloid beta peptides
<p>Supplementary information for article.</p> <ol> <li>Raman dataset for 473 nm, 633 nm and 785 nm</li> <li>Conversion template from Raman spectrum to binary OIT tag</li> <li>Screening template</li> </ol> <p>"Raman-derived identification tags for mutation screening in amyloid beta peptides" by Phuong Hoang, Niveen M. Khashab is licensed under a <a href="http://creativecommons.org/licenses/by-nc-nd/4.0/">Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License</a>.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.