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10 results for “Phormia”
Genome and developmental transcriptome of the forensically important blowfly, <em>Phormia Regina</em>
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Data from: Phormia regina allele report
<p>Genetic structure of blow fly (Diptera: Calliphoridae) populations has remained elusive regardless of high relatedness within wild-caught samples. The aims of this research were to determine if the implementation of a high-resolution spatiotemporal sampling design would reveal latent genetic structure among blow fly populations and to elucidate environmental impacts on structure. Adult female black blow flies, <em>Phormia regina </em>(Meigen), were collected from nine urban parks in Indiana, USA over three years. Fly gut DNA was genotyped at six microsatellite loci, with subsequent amplification and sequencing of vertebrate mitochondrial DNA from the same source. Flies were also screened for vertebrate fecal metabolites. Latent clustering revealed four genetic groups which were interpreted as 11 distinct temporal populations. An analysis of molecular variance of temporal populations revealed stronger genetic differentiation (F<sub>ST</sub> = 0.048, F'<sub>ST</sub> = 0.664) relative to geographic populations (F<sub>ST</sub> = 0.009, F'<sub>ST</sub> = 0.241). Mean kinship within temporal populations was higher than expected in a panmictic population (<em>R </em>= 0.032 ± 0.088). Weather conditions (i.e., wind speed, precipitation, humidity, temperature) and vertebrate resource availability in the local environment significantly impacted the observed genetics of <em>P. regina. </em>Twenty-five vertebrate species were detected from flies, and 16% of flies collected in 2016 – 2017 tested positive for vertebrate feces, suggesting many varied resources are important for maintaining high gene flow among geographic populations. A complex interplay between biotic and abiotic factors, as well as the flies' own extensive dispersal abilities, seems to drive the strong temporal structure of this species in the Midwestern US.</p>
Data from: Phormia regina allele report
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Supplementary figure 2 from: Jordaens K, Sonet G, Braet Y, de Meyer M, Backeljau T, Goovaerts F, Bourguignon L, Desmyter S (2013) DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae). ZooKeys 365: 149-174. https://doi.org/10.3897/zookeys.365.6202
Supplementary figure 2 - Neighbour-Joining tree (p-distances) of a 404 bp (229 bp without indels) fragment of the nuclear internal transcribed spacer 2 (ITS2). Bootstrap values ≥ 70% are shown at the nodes. N gives the number of specimens of that haplotype. EU = Phormia regina haplotypes from W Europe; NA = Phormia regina haplotypes from N America.
Supplementary figure 1 from: Jordaens K, Sonet G, Braet Y, de Meyer M, Backeljau T, Goovaerts F, Bourguignon L, Desmyter S (2013) DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae). ZooKeys 365: 149-174. https://doi.org/10.3897/zookeys.365.6202
Supplementary figure 1 - Neighbour-Joining tree (p-distances) of a 350 bp (A) and of a 251 bp (B) fragment of the mitochondrial 16S gene. Bootstrap values ≥ 70% are shown at the nodes. N gives the number of specimens of that haplotype. EU = Phormia regina haplotypes from W Europe; NA = Phormia regina haplotypes from N America.
Figure 4 from: Jordaens K, Sonet G, Braet Y, de Meyer M, Backeljau T, Goovaerts F, Bourguignon L, Desmyter S (2013) DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae). ZooKeys 365: 149-174. https://doi.org/10.3897/zookeys.365.6202
Figure 4 - Neighbour-Joining tree (p-distances) of a 512 bp fragment of the mitochondrial cytochrome b (cyt b) gene. Bootstrap values ≥ 70% are shown at the nodes. N gives the number of specimens of that haplotype. EU = Phormia regina haplotypes from W Europe; NA = Phormia regina haplotypes from N America.
Figure 3 from: Jordaens K, Sonet G, Braet Y, de Meyer M, Backeljau T, Goovaerts F, Bourguignon L, Desmyter S (2013) DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae). ZooKeys 365: 149-174. https://doi.org/10.3897/zookeys.365.6202
Figure 3 - Neighbour-Joining tree (p-distances) of a 472 bp fragment of the mitochondrial cytochrome c oxidase subunit II (COII) gene. Bootstrap values ≥ 70% are shown at the nodes. N gives the number of specimens of that haplotype. EU = Phormia regina haplotypes from W Europe; NA = Phormia regina haplotypes from N America.
Figure 2 from: Jordaens K, Sonet G, Braet Y, de Meyer M, Backeljau T, Goovaerts F, Bourguignon L, Desmyter S (2013) DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae). ZooKeys 365: 149-174. https://doi.org/10.3897/zookeys.365.6202
Figure 2 - Neighbour-Joining tree (p-distances) of a 655 bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene. Bootstrap values ≥ 70% are shown at the nodes. N gives the number of specimens of that haplotype. EU = Phormia regina haplotypes from W Europe; NA = Phormia regina haplotypes from N America.
Figure 1 from: Jordaens K, Sonet G, Braet Y, de Meyer M, Backeljau T, Goovaerts F, Bourguignon L, Desmyter S (2013) DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae). ZooKeys 365: 149-174. https://doi.org/10.3897/zookeys.365.6202
Figure 1 - Lateral (top) and dorsal (bottom) view of the male copulatory organs of Phormia regina from W Europe (left) and N America (right) with a detail of the penis (middle).
Supplementary figure 3 from: Jordaens K, Sonet G, Braet Y, de Meyer M, Backeljau T, Goovaerts F, Bourguignon L, Desmyter S (2013) DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae). ZooKeys 365: 149-174. https://doi.org/10.3897/zookeys.365.6202
Supplementary figure 3 - Neighbour-Joining tree (p-distances) of a 633 bp fragment of the nuclear 28S gene. Bootstrap values ≥ 70% are shown at the nodes. N gives the number of specimens of that haplotype. EU = Phormia regina haplotypes from W Europe; NA = Phormia regina haplotypes from N America.
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