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Dataset results
17 results for “SBF-SEM”
SBF-SEM datasets related to the manuscript "Trans-cellular tunnels induced by the fungal pathogen Candida albicans facilitate invasion through successive epithelial cells without host damage" by Lachat et al, 2022.
<p>11 serial block face- scanning electron microscopy (SBF-SEM) datasets described in the manuscript "Trans-cellular tunnels induced by the fungal pathogen Candida albicans facilitate invasion through successive epithelial cells without host damage" by Lachat et al, 2022.</p> <p>Resolution: 10 nm x,y, 100 nm Z.</p> <p>Datasets description and quantification can be found in the Supplementary information.</p>
SBF-SEM of the Trichuris muris Stichosome
<p>This image stack is a Serial Blockface Scanning Electron Microscopy (SBF-SEM) acquisition of the <em>Trichuris muris</em> stichosome. Structures of interest included within the data are the stichosome, bacillary pores and cells, sensory neurons and cuticular inflations.</p> <p>The worm was taken from a mouse host (Severe Combined Immunodeficient on a BALB/c background), and sample preparation was carried out according to Deerinck et al. (2010).</p> <p>Imaging was carried out on a Quanta 250 FEG (Thermo Fisher Scientific, Massachusetts), with the blockface recorded at 3.8kV, spot size 3.5 and pressure of 0.4Torr. Pixel size of the lateral plain was 12 nm. Slice thickness was 100 nm.</p> <p> </p> <p></p> <p><span>Deerinck, T. <em>et al.</em> Enhancing Serial Block-Face Scanning Electron Microscopy to enable high resolution 3-D nanohistology of cells and tissues. <em>Microsc. Microanal.</em> <strong>16</strong>, 1138–1139 (2010).</span></p>
Serial section images of Oikopleura dioica juvenile using SBF-SEM
<p>The larvacean, <i>Oikopleura dioica</i> is a planktonic chordate, which is an emerging model organism with short life cycle of 5 days and belongs to tunicates (urochordates). Organ formation in the trunk proceeds in seven hours form hatching of tailbud larvae at three hours after fertilization (hpf) to completion of organ formation in fully functional juveniles that start feeding at 10 hpf and are just miniature of adult form. The dataset is serial section images of Oikopleura dioica juvenile that were obtained using SBF-SEM (Serial block face scanning electron microscopy). The 1961 TIFF images were compressed to a zip file.</p> <p>The juveniles were fixed at 10.5 hours post fertilization, 30 min after the tail shift (Nishida, H., 2008 Development of the appendicularian Oikopleura dioica: culture, genome, and cell lineages. Dev. Growth Differ. 50, S239–S256.). The dataset would be used as a basic morphological data of this animal to explore organ structures and cellular composition at electron microscopic level.</p> <p>This data is related to the paper, 3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM. (Scientific Reports, 2021, 11, 4833) (https://www.nature.com/articles/s41598-021-83706-y)</p>
SBF-SEM Dataset of Locust Lobula
<p>SBF-SEM dataset of locust lobula, of 500 sections at 60nm thickness. Images are 6000x6000 pixels at 8.7nm resolution. </p>
SBF-SEM Dataset of Guinea Pig Adult Psoas
<p>Multiple SBF-SEM datasets from 3 Duncan hartley guinea pig adult psoas muscle. Parameters for each of the datasets are attached as a spreadsheet, including nm resolution, image size, number of sections and section thickness. </p> <p> </p>
Serial section images of Oikopleura dioica juvenile using SBF-SEM
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Serial section images of Oikopleura dioica larva using SBF-SEM
<p>The larvacean, Oikopleura dioica is a planktonic chordate, which is an emerging model organism with short life cycle of 5 days and belongs to tunicates (urochordates). Organ formation in the trunk proceeds in seven hours form hatching of tailbud larvae at three hours after fertilization (hpf) to completion of organ formation in fully functional juveniles that start feeding at 10 hpf and are just miniature of adult form. The dataset is serial section images of Oikopleura dioica juvenile that were obtained using SBF-SEM (Serial block face scanning electron microscopy). The 1812 TIFF images were compressed to a zip file.</p> <p>The larva were fixed at 3 hours post fertilization, 10 min after hatching (Nishida, H., 2008 Development of the appendicularian Oikopleura dioica: culture, genome, and cell lineages. Dev. Growth Differ. 50, S239–S256.). The dataset would be used as a basic morphological data of this animal to explore organ structures and cellular composition at electron microscopic level.</p> <p>This data is related to the paper, 3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM. (Scientific Reports, 2021, 11, 4833) (https://www.nature.com/articles/s41598-021-83706-y).</p>
3D Reconstruction of guinea pigs cardiac SBF-SEM datasets
<p>3D Reconstruction of guinea pigs cardiac SBF-SEM datasets:</p> <p>Total three datasets were reconstructed in 3D. The serial sections of the dataset are put in the file name. </p> <p>Reconstructions were done using MIB and AMIRA</p>
SBF-SEM Dataset of Guinea Pig Fetal Day 50-54 Psoas Muscle
<p>Multiple SBF-SEM datasets of Duncan Hartley guinea pigs at fetal day 50-54 from psoas muscle. Parameters for each of the datasets are attached as a spreadsheet, including nm resolution, image size, number of sections and section thickness. </p>
SBF-SEM Dataset of Guinea Pig Adult Soleus
<p>Multiple SBF-SEM datasets from 3 Duncan hartley guinea pig adult soleus muscle. Parameters for each of the datasets are attached as a spreadsheet, including nm resolution, image size, number of sections and section thickness. </p>
SBF-SEM Dataset of Guinea Pig Fetal Day 55-59 Soleus Muscle
<p>Multiple SBF-SEM datasets of Duncan Hartley guinea pigs at fetal day 55-59 from soleus muscle. Parameters for each of the datasets are attached as a spreadsheet, including nm resolution, image size, number of sections and section thickness. </p>
SBF-SEM Dataset of Guinea Pig Fetal Day 55-59 Psoas Muscle
<p>Multiple SBF-SEM datasets of Duncan Hartley guinea pigs at fetal day 55-59 from psoas muscle. Parameters for each of the datasets are attached as a spreadsheet, including nm resolution, image size, number of sections and section thickness. </p>
SBF-SEM Dataset of Guinea Pig Fetal Day 60-64 Psoas Muscle
<p>Multiple SBF-SEM datasets from 3 Duncan hartley guinea pig fetal day 60-64 psoas muscle. Parameters for each of the datasets are attached as a spreadsheet, including nm resolution, image size, number of sections and section thickness. </p>
SBF-SEM Dataset of Guinea Pig Fetal Day 60-64 Soleus Muscle
<p>Multiple SBF-SEM datasets from 3 Duncan hartley guinea pig fetal day 60-64 soleus muscle. Parameters for each of the datasets are attached as a spreadsheet, including nm resolution, image size, number of sections and section thickness. </p>
SBF-SEM Dataset of Guinea Pig Fetal Day 50-54 Soleus Muscle
<p>Multiple SBF-SEM datasets of Duncan Hartley guinea pigs at fetal day 50-54 from soleus muscle. Parameters for each of the datasets are attached as a spreadsheet, including nm resolution, image size, number of sections and section thickness. </p>
Serial section images of Oikopleura dioica larva using SBF-SEM
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SBF-SEM Annotations of the CP Frnd
<p>SBF-SEM stacks and annotations of the CP frnd</p>
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