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Fig. 4 in Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response

Fig. 4. In vitro enzymatic assays of recombinant SaNES/LIS. GC-MS identification of (E)-nerolidol (A) and linalool (B) derived from FPP and GPP under action of recombinant SaNES/LIS, respectively. m/z, mass-to-charge ratio.

Fig. 3 in Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response

Fig. 3. Subcellular localization of SaNES/LIS in Arabidopsis mesophyll protoplasts. The full-length coding region of SaNES/LIS was fused to the YFP reporter gene in the vector pSAT6-EYFP-N1 to produce the pSAT6- SaNES/LIS construct. The fusion construct were then transferred into Arabidopsis protoplasts and analyzed by confocal laser scanning microscopy for YFP expression. The yellow columns show YFP fluorescence, the blue column shows chlorophyll auto-fluorescence, the merged column shows combined YFP fluorescence and chlorophyll auto-fluorescence, and the transmission column shows light-microscopy images of the intact protoplasts. (A) Control pSAT6-YFP construct. (B, C) pSAT6-SaNES/LIS (expressed in plastids and the cytosol, respectively). Arrows 1 and 2 indicate granular fluorescence and diffuse fluorescence, respectively in the plastid. Fluorescence was excited for YFP at 514 nm and for Chl at 543 nm. Scale bar = 10 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 6 in Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response

Fig. 6. In vivo characterization of the SaNES/LIS gene, which was transiently expressed in tobacco leaves by Agrobacterium-mediated infiltration. Compounds were analyzed three days post-infiltration by GC-MS. New product peaks were observed in contrast to controls and were identified by comparing mass spectra with library data. Leaves of tobacco infiltrated by pBI121 alone were used as the control. Peak 1, linalool; peak 2, (E)-nerolidol. m/z, mass-to-charge ratio.

Fig. 2 in Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response

Fig. 2. Phylogenetic tree of SaNES/LIS protein within representative samples of known plant TPSs. The neighbor-joining tree was drawn using MEGA 7.0 (Kumar et al., 2016). Bootstrap values from 1000 replicates were used to assess the robustness of the trees.

Fig. 1 in Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response

Fig. 1. Protein sequence alignment of SaNES/LIS with proteins from Vitis vinifera and Magnolia champaca. The RRX8W motif, the Asp-rich domain DDXXD, and the NSE/DTE motif are indicated. These are highly conserved in plant TPSs. Completely conserved residues are shaded in black, and similar residues are shaded in light grey. Dashes indicate gaps introduced to maximize sequence alignment.

Fig. 5 in Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response

Fig. 5. Plots of SaNES/LIS reaction rates versus FPP and GPP concentration. The FPP and GPP saturation curves were determined at pH 7.4 and the Michaelis-Menten model was used to determine Km and Vmax. Each data point was determined from triplicate measurements.

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Figure 3 from: Butaud J-F (2015) Reinstatement of the Loyalty Islands Sandalwood, Santalum austrocaledonicum var. glabrum (Santalaceae), in New Caledonia. PhytoKeys 56: 111-126. https://doi.org/10.3897/phytokeys.56.5924

Figure 3 - Fruit of Santalum austrocaledonicum var. glabrum on Ouvéa atoll in January 2015 (specimen Butaud 3414).

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Figure 2 from: Butaud J-F (2015) Reinstatement of the Loyalty Islands Sandalwood, Santalum austrocaledonicum var. glabrum (Santalaceae), in New Caledonia. PhytoKeys 56: 111-126. https://doi.org/10.3897/phytokeys.56.5924

Figure 2 - Flowers of Santalum austrocaledonicum var. glabrum on Ouvéa atoll in January 2015 (specimen Butaud 3414).

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Figure 1 from: Butaud J-F (2015) Reinstatement of the Loyalty Islands Sandalwood, Santalum austrocaledonicum var. glabrum (Santalaceae), in New Caledonia. PhytoKeys 56: 111-126. https://doi.org/10.3897/phytokeys.56.5924

Figure 1 - Distribution map of New Caledonian varieties of Santalum austrocaledonicum based on the specimens examined.

Fig. 7 in Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response

Fig. 7. Expression levels of the SaNES/LIS gene in different tissues.

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Fig. 8 in Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response

Fig. 8. qRT-PCR expression analysis of SaNES/LIS from S. album subjected to different stresses. Six-month-old seedlings were sprayed with 1 mM MeJA (A) or exposed to 4 ◦C, 38 ◦C, strong illumination (approx. 250 μmol m 2 s 1) or drought for one week (B). Three measurements were averaged from the results of three replicated experiments. Significant differences were analyzed by a student's t-test (*P <0.05, **P <0.01 and ***P <0.001).