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12 results for “Shiraiaceae”
FIGURE 4 in Shiraiaceae, new family of Pleosporales (Dothideomycetes, Ascomycota)
FIGURE 4. Sexual stage of Shiraia bambusicola (epitype). (Figs A–D, F–M, O–P light microscopy and Figs E, N SEM). A. Ascostroma with yellow ascospore mass. B. Cross section of ascostroma. Note the sexual locules lining the periphery (a) and the asexual locules near the centre (b). The outer part is pink, while the inner part is white to light pink. C. Cross section of locules (fluorescent microscopy stained in Hoechst 33258). D. Vertical section of ascostroma. E. Tissue of locule wall. Note the cells of textura angularis at the inside fusing with cells of textura intricata at the outside. F. Pseudoparaphyses (cotton blue reagent staining). G. Asci and pseudoparaphyses. H–K. Asci. L–M. Apices of asci showing ocular chamber. N–P. Ascospores (P stained in lactic acid phenol Medan). Scale bars: A = 1 cm; B = 1.5 cm; C–D = 100 µm; E = 10 µm; F = 20 µm; G–K = 50 µm; L–M = 20 µm; N–P = 10 µm.
FIGURE 3 in Shiraiaceae, new family of Pleosporales (Dothideomycetes, Ascomycota)
FIGURE 3. Asexual state of Shiraia bambusicola (epitype). (Figs A–B, D–E, J–N light microscopy and Figs C, F–I SEM) A. Ascostroma on bamboo branches. Note that the ascostromata form at the end of branches, but below the leaf petioles. B. Ascostromata with yellow conidium mass. C. Cross section of ascostroma. Note the leaf sheath of the host bamboo with green drawing and asexual locule (arrowed). D–E. Asexual locule (D shows release of the conidia, E fluorescent microscopy stained in Hoechst 33258). F–G. Conidia growing on the wall of the conidiomata. H. Cells of the asexual locule. I. Conidium on short conidiophore. J–L. Conidia. M– N. Colony (face and reverse views) on PDA after 14 days at 25°C. Scale bars: A = 2 cm; B = 1 cm; C = 1 mm; D = 200 µm; E = 300 µm; F–G =20 µm; H–L = 10 µm.
FIGURE 1 in Shiraiaceae, new family of Pleosporales (Dothideomycetes, Ascomycota)
FIGURE 1. Phylogram constructed using MP analysis of 28S nrDNA nucleotide sequences. Bootstrap support values>50% are shown above the branch. Dothidea sambuci is the outgroup taxon. The original isolate numbers are noted after the species names.
FIGURE 2 in Shiraiaceae, new family of Pleosporales (Dothideomycetes, Ascomycota)
FIGURE 2. Shiraia bambusicola (holotype) A. Surface of stroma. B–C. Section of stroma in cotton blue reagent showing arrangement of locules. D. Section of locule wall. E–F. Six-spored asci and pseudoparaphyses. G. Ascus. H. Conidium. I. Muriform ascospore. Scale bars: C = 200 µm, D–I = 40 µm.
Figure 8 from: Dai D-Q, Wijayawardene NN, Tang L-Z, Liu C, Han L-H, Chu H-L, Wang H-B, Liao C-F, Yang E-F, Xu R-F, Li Y-M, Hyde KD, Bhat DJ, Cannon PF (2019) Rubroshiraia gen. nov., a second hypocrellin-producing genus in Shiraiaceae (Pleosporales). MycoKeys 58: 1-26. https://doi.org/10.3897/mycokeys.58.36723
Figure 8 Hypocrella bambusae (K(M)52469, isotype, images are accredited to the Royal Botanic Gardens, Kew) A, C fruiting bodies on inflorescence of bamboo B vertical section of stromata showing the perithecia locating D herbarium envelope E filiform ascospores F asci with caps (Staining by cotton blue). Scale bars: 5 mm (A), 200 μm (B), 2 mm (C), 20 μm (E, F).
Figure 7 from: Dai D-Q, Wijayawardene NN, Tang L-Z, Liu C, Han L-H, Chu H-L, Wang H-B, Liao C-F, Yang E-F, Xu R-F, Li Y-M, Hyde KD, Bhat DJ, Cannon PF (2019) Rubroshiraia gen. nov., a second hypocrellin-producing genus in Shiraiaceae (Pleosporales). MycoKeys 58: 1-26. https://doi.org/10.3897/mycokeys.58.36723
Figure 7 Rubroshiraia bambusae (HKAS102255, holotype) A fruiting bodies B, C surface of ascostromata showing the openings of ostiole D vertical section of ascostromata E, F vertical section of locule G peridium of locule H asci and pseudoparaphyses I asci and asci ocular chamber J ascospores K, L immature asci. Scale bars: 1 cm (A), 25 mm (B), 2 mm (C, D), 500 μm (E, F), 200 μm (G), 50 μm (H–L).
Figure 5 from: Dai D-Q, Wijayawardene NN, Tang L-Z, Liu C, Han L-H, Chu H-L, Wang H-B, Liao C-F, Yang E-F, Xu R-F, Li Y-M, Hyde KD, Bhat DJ, Cannon PF (2019) Rubroshiraia gen. nov., a second hypocrellin-producing genus in Shiraiaceae (Pleosporales). MycoKeys 58: 1-26. https://doi.org/10.3897/mycokeys.58.36723
Figure 5 The UV spectrum of the standards and of hypocrellin A and B from the samples (Shiraia bambusicolaHKAS 102253 and Rubroshiraia bambusaeHKAS 102255) were recorded in alcohol at room temperature. HA: hypocrellin A, HB: hypocrellin B.
Figure 6 from: Dai D-Q, Wijayawardene NN, Tang L-Z, Liu C, Han L-H, Chu H-L, Wang H-B, Liao C-F, Yang E-F, Xu R-F, Li Y-M, Hyde KD, Bhat DJ, Cannon PF (2019) Rubroshiraia gen. nov., a second hypocrellin-producing genus in Shiraiaceae (Pleosporales). MycoKeys 58: 1-26. https://doi.org/10.3897/mycokeys.58.36723
Figure 6 Shiraia bambusicolaA–J sexual morph A fruiting bodies (HKAS102253, HKAS102254, HKAS102257, HKAS102261, HKAS102262) B–J photographs from material HKAS102253 B Surface of ascostromata showing the dark openings of ostiole C vertical section of ascostromata D vertical section of locule E pseudoparaphyses F, G asci (G Showing the fissitunicate asci) H–J ascospores K–M asexual morph K vertical section of asexual locules L–M conidia. Scale bars: 2 cm (A), 5 mm (B), 1 mm (C), 100 μm (D, K), 50 μm (F, G), 20 μm (H–J, L, M).
Figure 4 from: Dai D-Q, Wijayawardene NN, Tang L-Z, Liu C, Han L-H, Chu H-L, Wang H-B, Liao C-F, Yang E-F, Xu R-F, Li Y-M, Hyde KD, Bhat DJ, Cannon PF (2019) Rubroshiraia gen. nov., a second hypocrellin-producing genus in Shiraiaceae (Pleosporales). MycoKeys 58: 1-26. https://doi.org/10.3897/mycokeys.58.36723
Figure 4 Hypocrellin A and hypocrellin B HPLC-UV profiles (265 nm) of standards and stromatal HPLC-UV profiles (265 nm) of specimens of Shiraia bambusicola (HKAS 102253) (II) and Rubroshiraia bambusae (HKAS 102255) (III) and DAD spectra of major metabolites.
Figure 3 from: Dai D-Q, Wijayawardene NN, Tang L-Z, Liu C, Han L-H, Chu H-L, Wang H-B, Liao C-F, Yang E-F, Xu R-F, Li Y-M, Hyde KD, Bhat DJ, Cannon PF (2019) Rubroshiraia gen. nov., a second hypocrellin-producing genus in Shiraiaceae (Pleosporales). MycoKeys 58: 1-26. https://doi.org/10.3897/mycokeys.58.36723
Figure 3 Maximum likelihood phylogenetic tree generated from RAxML (GTR+G model), based on combined LSU and ITS sequences data. ML and MP values (MLBP/MPBP) (> 50%), resulting from 1000 bootstrap replicates, are given at the nodes. The original isolate numbers' codes are noted after the species names. The tree is rooted to Pleospora herbarum (CBS 191.86). Ex-type or ex-epitype strains are in bold. Newly generated strains are in red.
Figure 2 from: Dai D-Q, Wijayawardene NN, Tang L-Z, Liu C, Han L-H, Chu H-L, Wang H-B, Liao C-F, Yang E-F, Xu R-F, Li Y-M, Hyde KD, Bhat DJ, Cannon PF (2019) Rubroshiraia gen. nov., a second hypocrellin-producing genus in Shiraiaceae (Pleosporales). MycoKeys 58: 1-26. https://doi.org/10.3897/mycokeys.58.36723
Figure 2 Maximum likelihood phylogenetic tree generated from RAxML (GTR+G model), based on combined LSU, SSU, TEF1 and RPB2 sequences data. ML values (MLBP) (> 50%), resulting from 1000 bootstrap replicates and Bayesian posterior probabilities (BYPP) greater than 0.90, are given at the nodes. The original isolate numbers' codes are noted after the species names. The tree is rooted to Dothidea insculpta (CBS 189.58). Ex-type or ex-epitype strains are in bold. Newly generated strains are in red and the new genus is in yellow background.
Figure 1 from: Dai D-Q, Wijayawardene NN, Tang L-Z, Liu C, Han L-H, Chu H-L, Wang H-B, Liao C-F, Yang E-F, Xu R-F, Li Y-M, Hyde KD, Bhat DJ, Cannon PF (2019) Rubroshiraia gen. nov., a second hypocrellin-producing genus in Shiraiaceae (Pleosporales). MycoKeys 58: 1-26. https://doi.org/10.3897/mycokeys.58.36723
Figure 1 Chemical structures of hypocrellin A and hypocrellin B. A hypocrellin A B hypocrellin B.
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