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1,276 results for “Stromal cells”
Data: DEAE-Dextran enhances the lentiviral transduction of primary human mesenchymal stromal cells from all major tissue sources without affecting their proliferation and phenotype
<p>This data set includes all the raw data collected for the following article: "DEAE-Dextran enhances the lentiviral transduction of primary human mesenchymal stromal cells from all major tissue sources without affecting their proliferation and phenotype"</p>
Storage conditions affect the composition of the lyophilized secretome of multipotent mesenchymal stromal cells - graphs
<p>Figures for artcile Rogulska, O., Vackova, I., Prazak, S. <em>et al.</em> Storage conditions affect the composition of the lyophilized secretome of multipotent mesenchymal stromal cells. <em>Sci Rep</em> 14, 10243 (2024). https://doi.org/10.1038/s41598-024-60787-z</p> <p><span>Fig. 1. Preservation of the growth factors and cytokines in the lyophilized MSC-derived conditioned medium after 3 months of storage at different temperatures (N=3). Data is presented as Median (Q1; Q3). * - the values are significantly (p<0.05) lower compared to the -80°C group; # - the values are significantly (p<0.05) lower compared to the -20°C group. The values are related to non-lyophilized MSC-sec stored for the same time (3 months) in a frozen state at −80°C.</span></p> <p><span> </span><span>Fig. 2. The preservation of growth factors and cytokines in the lyophilized MSC-derived conditioned medium after 30 months of storage at different temperatures (N=3). Data is presented as Median (Q1; Q3). * - the values are significantly (p<0.05) lower compared to the -80°C group; # - the values are significantly (p<0.05) lower compared to the -20°C group; ° - the values are significantly (p<0.05) lower compared to the 4°C group. The values are related to non-lyophilized MSC-sec stored for the same time (30 months) in a frozen state at −80°C.</span></p> <p><span> </span><span>Fig. 3. The preservation levels of the whole lyophilized MSC-sec cocktail, determined as the median of the preservation values for each of the MSC-sec components. The v</span><span>alues are related to non-lyophilized MSC-sec stored for the same time in a frozen state at −80°C.</span></p>
Data from: MET receptor activation by stromal cells serves as promising target in melanoma brain metastases
<p>Supplementary tables to our recent manuscript: MET receptor activation by stromal cells serves as promising target in melanoma brain metastases</p>
Distinct Stromal Cell Populations Define the B-cell Acute Lymphoblastic Leukemia Microenvironment
<p>Processed single-cell RNA-seq from the study </p> <ul> <li>10X Genomics CellRanger output (barcodes.tsv, genes.tsv, matrix.mtx) for each each sample</li> <li>Metadata</li> <li>Seurat object of the integrated scRNAseq dataset</li> <li>Xenium object of the spatial transcriptomic data</li> </ul> <p>Distinct Stromal Cell Populations Define the B-cell Acute Lymphoblastic Leukemia Microenvironment</p> <p>Mauricio N. Ferrao Blanco<sup>1</sup>, Bexultan Kazybay<sup>1</sup>, Mirjam Belderbos<sup>1</sup>, Olaf Heidenreich<sup>1</sup>, Hermann Josef Vormoor<sup>1,2</sup></p> <p><sup>1 </sup>Princess Máxima Center for Pediatric Oncology, Utrecht, the Netherlands</p> <p><sup>2 </sup>University Medical Center Utrecht, Utrecht, the Netherlands</p> <p><strong>Abstract</strong></p> <p>The bone marrow microenvironment plays a critical role in B-cell acute lymphoblastic leukemia (B-ALL) progression, yet its cellular heterogeneity remains poorly understood. Using single-cell RNA sequencing on patient-derived of bone marrow aspirates from pediatric B-ALL patients, we identified two distinct mesenchymal stromal cell (MSC) populations: early mesenchymal progenitors and adipogenic progenitors. Spatial transcriptomic analysis further revealed the localization of these cell types and identified a third stromal population, osteogenic-lineage cells, exclusively present in the bone biopsy. Functional <em>ex vivo</em> assays using sorted stromal populations derived from B-ALL patient bone marrow aspirates demonstrated that both early mesenchymal and adipogenic progenitors secrete key niche-supportive factors, including CXCL12 and Osteopontin, and support leukemic cell survival and chemoresistance. Transcriptomic profiling revealed that B-ALL cells interact differently with stromal subtypes. Notably, adipogenic progenitors, but not early mesenchymal progenitors, provide support to leukemic cells through interleukin-7 and VCAM1 signaling. Stromal cells from B-ALL patients exhibited an enhanced adipogenic differentiation capacity compared to healthy controls. Moreover, co-culture experiments showed that B-ALL cells induce adipogenic differentiation in healthy MSCs through a cell contact-dependent mechanism. Adipogenic progenitors were also enriched in relapse samples, implicating them in disease progression. These findings highlight the complexity of the B-ALL microenvironment and identify different specialized stromal niches with which the leukemic cells can engage.</p> <p> </p> <p> </p> <p> </p>
Safety and Efficacy of Mesenchymal Stromal Cells Mitochondria Transplantation as a Cell-Free Therapy for Osteoarthritis
<p><strong><span>Abstract</span></strong></p> <p><strong><span>Objective</span></strong></p> <p><span>The inflammatory responses from synovial fibroblasts and macrophages</span><span> and the mitochondrial dysfunction in chondrocytes<span> leading to oxidative stress, disrupt extracellular matrix (ECM) homeostasis</span> and accelerate the deterioration process of articular cartilage<span> in osteoarthritis (OA).<span> </span>In the last years, it has been proposed that mesenchymal stromal cells (MSC) transfer their functional mitochondria to damaged cells in response to cellular stress, becoming one of the mechanisms underpinning their therapeutic effects. </span>Therefore, we hypothesize that a novel cell-free treatment for OA could involve direct mitochondria transplantation, leading to the restoration of both cellular and mitochondrial homeostasis.</span></p> <p><strong><span>Methods</span></strong></p> <p><span>Mitochondria<span> were isolated from Umbilical Cord (UC)-MSC (Mito-MSC) and characterized based on their morphology, phenotype, functions, and their ability to be </span>internalized by <span>different articular cells. Furthermore, </span>the transcriptional changes following mitochondrial uptake by chondrocytes were evaluated using an Affymetrix analysis, Lastly, <span>the dose dependance therapeutic efficacy, biodistribution and immunogenicity of Mito-MSC w</span>ere assessed<span> in vivo, </span>through an intra-articular injection <span>in male C57BL6 mice in a </span>collagenase-induced OA <span>(CIOA) </span>model.</span></p> <p><strong><span>Results</span></strong></p> <p><span>Our findings demonstrate the functional integrity of Mito-MSC and their ability to be efficiently <span>transferred into chondrocytes, synovial macrophages, and synovial fibroblasts. </span>Moreover, the transcriptomic analysis showed the upregulation of genes involved in stress such as DNA reparative machinery and<span> inflammatory antiviral responses</span>.<span> </span>Finally, <span>Mito-MSC transplantation </span>yielded significant reductions<span> in joint mineralization, </span>a hallmark of OA progression<span>, </span>as well as improvements in OA-related histological signs, with the lower dose exhibiting better therapeutic efficacy. <span><span> </span></span>Furthermore, Mito-MSC were detected within the knee joint for up to 24 hours post-injection without eliciting an inflammatory response in CIOA mice. </span></p> <p><strong><span>Conclusion</span></strong></p> <p><span><span> </span>Collectively, our results reveal that mitochondria derived from MSC are transferred to key articular cells and are retained in the joint without generating an inflammatory immune response mitigating articular cartilage degradation in OA, probably through a restorative effect trigger by the stress antiviral response within OA chondrocytes.</span></p>
Data: Computed tomography lacks sensitivity to image gold labelled mesenchymal stromal cells in vivo as evidenced by multispectral optoacoustic tomography.
<p>This data set includes all the raw data collected for the following article: "Computed tomography lacks sensitivity to image gold labelled mesenchymal stromal cells in vivo as evidenced by multispectral optoacoustic tomography."</p>
DATA: Comparison between optical tissue clearing methods for detecting administered mesenchymal stromal cells in mouse lungs
<p>This data set includes all the raw data collected for the following article: "Comparison between optical tissue clearing methods for detecting administered mesenchymal stromal cells in mouse lungs".</p>
Data: Fate of intravenously administered umbilical cord mesenchymal stromal cells and interactions with the host's immune system
<p>This data set includes all the raw data collected for the following article: "Fate of intravenously administered umbilical cord mesenchymal stromal cells and interactions with the host's immune system".</p>
Raw Data for the article: Current Perspectives on Adult Mesenchymal Stromal Cell-Derived Extracellular Vesicles: Biological Features and Clinical Indications
<p>Extracellular vesicles (EVs) constitute one of the main mechanisms by which cells communicate with the surrounding tissue or at distance. Vesicle secretion is featured by most cell types, and adult mesenchymal stromal cells (MSCs) of different tissue origins have shown the ability to produce them. In recent years, several reports disclosed the molecular composition and suggested clinical indications for EVs derived from adult MSCs. The parental cells were already known for their roles in different disease settings in regulating inflammation, immune modulation, or transdifferentiation to promote cell repopulation. Interestingly, most reports also suggested that part of the properties of parental cells were maintained by isolated EV populations. This review analyzes the recent development in the field of cell-free therapies, focusing on several adult tissues as a source of MSC-derived EVs and the available clinical data from in vivo models.</p>
Single-cell RNA sequencing of human salivary gland derived mesenchymal stromal cells under cytokine treatment conditions
Open the record for dataset details and reuse information.
Bulk RNA sequencing of human mesenchymal stromal cells derived from labial salivary glands, bone marrow, and adipose
Open the record for dataset details and reuse information.
Raw Data for the article: Extracellular Vesicle-Derived microRNAs of Human Wharton's Jelly Mesenchymal Stromal Cells May Activate Endogenous VEGF-A to Promote Angiogenesis
<p>Despite low levels of vascular endothelial growth factor (VEGF)-A, the secretome of human Wharton's jelly (WJ) mesenchymal stromal cells (MSCs) effectively promoted proangiogenic responses in vitro, which were impaired upon the depletion of small (~140 nm) extracellular vesicles (EVs). The isolated EVs shared the low VEGF-A profile of the secretome and expressed five microRNAs, which were upregulated compared to fetal dermal MSC-derived EVs. These upregulated microRNAs exclusively targeted the <em>VEGF-A</em> gene within 54 Gene Ontology (GO) biological processes, 18 of which are associated with angiogenesis. Moreover, 15 microRNAs of WJ-MSC-derived EVs were highly expressed (Ct value ≤ 26) and exclusively targeted the thrombospondin 1 (<em>THBS1</em>) gene within 75 GO biological processes, 30 of which are associated with the regulation of tissue repair. The relationship between predicted microRNA target genes and WJ-MSC-derived EVs was shown by treating human umbilical-vein endothelial cells (HUVECs) with appropriate doses of EVs. The exposure of HUVECs to EVs for 72 h significantly enhanced the release of VEGF-A and THBS1 protein expression compared to untreated control cells. Finally, WJ-MSC-derived EVs stimulated in vitro tube formation along with the migration and proliferation of HUVECs. Our findings can contribute to a better understanding of the molecular mechanisms underlying the proangiogenic responses induced by human umbilical cord-derived MSCs, suggesting a key regulatory role for microRNAs delivered by EVs.</p>
Raw Data for the article: Human Amnion-Derived Mesenchymal Stromal Cells: A New Potential Treatment for Carbapenem-Resistant Enterobacterales in Decompensated Cirrhosis
<p><strong>Background: </strong>Spontaneous bacterial peritonitis (SBP) is a severe and often fatal infection in patients with decompensated cirrhosis and ascites. The only cure for SBP is antibiotic therapy, but the emerging problem of bacterial resistance requires novel therapeutic strategies. Human amniotic mesenchymal stromal cells (hA-MSCs) possess immunomodulatory and anti-inflammatory properties that can be harnessed as a therapy in such a context.</p> <p><strong>Methods: </strong>An in vitro applications of hA-MSCs in ascitic fluid (AF) of cirrhotic patients, subsequently infected with carbapenem-resistant Enterobacterales, was performed. We evaluated the effects of hA-MSCs on bacterial load, innate immunity factors, and macrophage phenotypic expression.</p> <p><strong>Results: </strong>hA-MSCs added to AF significantly reduce the proliferation of both bacterial strains at 24 h and diversely affect M1 and M2 polarization, C3a complement protein, and ficolin 3 concentrations during the course of infection, in a bacterial strain-dependent fashion.</p> <p><strong>Conclusion: </strong>This study shows the potential usefulness of hA-MSC in treating ascites infected with carbapenem-resistant bacteria and lays the foundation to further investigate antibacterial and anti-inflammatory roles of hA-MSC in in vivo models.</p>
Adipose-derived stromal cells preserve pancreatic islet function in a transplantable 3D bioprinted scaffold
<p><span>Intra-portal islet transplantation is the method of choice for treatment of insulin dependent type 1 diabetes, but its outcome is hindered by limited islet survival due to the immunological and metabolic stress post transplantation. Adipose-derived stromal cells (ASCs) promise to improve significantly the islet micro-environment but an efficient long-term delivery method has not been achieved. We therefore explore the potential of generating ASC enriched islet transplant structure by 3D bioprinting. Here, we fabricate a double-layered 3D bioprinted scaffold for islets and ASCs by using alginate-nanofibrillated cellulose bioink. We demonstrate the diffusion properties of the scaffold and report that human ASCs increase the islet viability, preserve the endocrine function, and reduce pro-inflammatory cytokines secretion <em>in vitro</em>. Intraperitoneal implantation of the ASCs and islets in 3D bioprinted scaffold improve the long-term function of islets in diabetic mice. Our data reveals an important role for ASCs on the islet micro-environment. We suggest a novel cell therapy approach of ASCs combined with islets in a 3D structure with a potential for clinical beta cell replacement therapies at extrahepatic sites. </span></p>
Generation of fluorescently labelled OP9 stromal cells for growing patient leukemic cells
<p>We added fluorescent labels to OP9 stromal cells to allow us to easily distinguish stromal cells from primary leukemia cells in co-culture growth conditions.</p>
Discovery proteomics by mass spectrometry comparing secreted proteins from young and old mouse lung mesenchymal stromal cells
<p>Lung mesenchymal stromal cells (L-MSCs) were isolated from the young ( 3 months) and old (22-24 months) mice (N = 4 each) following collagenase digestion of the lungs and anchorage-dependent growth. Culture media was collected from young and old L-MSCs (10^6 cells) grown in culture for 24 hrs in serum-free condition. Culture media was then centrifuged to remove any cellular debris. Five (5) ml of supernatants were concentrated using 3kDa Molecular weight cut-off filters, and 10 micrograms of proteins were <span><span><span><span><span>run as multiple MW 1D PAGE fractions using a standard </span><span>GeLC</span><span> approach with a </span><span>nano</span><span>-HPLC in-line with a </span><span>Velos</span><span> Pro Orbitrap MS. </span></span></span></span></span><span><span><span><span><span>Individual data files were searched using </span><span>Sequest</span><span>, the results were then combined for each sample, followed by grouping, filtering, and quantifying by normalized spectral counts. </span></span></span></span></span></p> <p> </p>
Dataset related to article "Systematic review and meta-analysis of preclinical studies testing mesenchymal stromal cells for traumatic brain injury"
<p>Mesenchymal stromal cells (MSCs) are widely used in preclinical models of traumatic brain injury (TBI). Results are promising in terms of neurological improvement but are hampered by wide variability in treatment responses. We made a systematic review and meta-analysis: 1) to assess the quality of evidence for MSC treatment in TBI rodent models; 2) to determine the effect size of MSCs on sensorimotor function, cognitive function and anatomical damage; 3) to identify MSC-related and protocol-related variables associated with greater efficacy; 4) to understand whether MSC manipulations boost therapeutic efficacy.</p> <p>The meta-analysis included 80 studies. After TBI, MSCs improved sensorimotor and cognitive deficits, and reduced anatomical damage. Stratified meta-analysis on sensorimotor outcome showed similar efficacy for different MSC sources and for syngeneic or xenogenic transplants. Efficacy was greater when MSCs were delivered in the first week post-injury, and when implanted directly into the lesion cavity. The greatest effect size was for cells embedded in matrices or for MSC-derivatives.</p> <p>MSC therapy is effective in preclinical TBI models, improving sensorimotor, cognitive and anatomical outcomes, with large effect sizes. These findings support clinical studies in TBI.</p> <p>The present dataset reports extrapolated data used for the meta-analysis</p>
Trellis Single-Cell Screening Reveals Stromal Regulation of Patient-Derived Organoid Drug Responses
<p>Patient-derived organoids (PDOs) can model personalized therapy responses, however current screening technologies cannot reveal drug response mechanisms or how tumor microenvironment cells alter therapeutic performance. To address this, we developed a highly-multiplexed mass cytometry platform to measure post translational modification (PTM) signaling, DNA-damage, cell-cycle activity, and apoptosis in >2,500 colorectal cancer (CRC) PDOs and cancer associated fibroblasts (CAFs) in response to clinical therapies at single-cell resolution. To compare patient- and microenvironment-specific drug responses in thousands of single-cell datasets, we developed <em>Trellis</em> — a highly-scalable, hierarchical tree-based treatment effect analysis method. Trellis single-cell screening revealed that on-target cell-cycle blockage and DNA-damage drug effects are common, even in chemorefractory PDOs. However, drug-induced apoptosis is rare, patient-specific, and aligns with cancer cell PTM signaling. We find that CAFs can regulate cancer cell plasticity — shifting proliferative stem cells to slow-cycling revival stem cells via YAP to protect cancer cells from chemotherapy.</p> <p> </p> <p>This repo contains the processed scRNA-seq Scanpy AnnData objects generated from the study. More information describing the data can be found at: https://github.com/TAPE-Lab/Ramos-et-al-Trellis</p>
A Prospective Study of Remestemcel-L, Ex-vivo Cultured Adult Human Mesenchymal Stromal Cells, for the Treatment of Pediatric Participants Who Have Failed to Respond to Steroid Treatment for Acute Graf
ClinicalTrials.gov study NCT02336230. IPD Sharing: NO. Countries: 1. Publications: 1.
Perinatal Arterial Stroke Treated With Stromal Cells Intranasally
ClinicalTrials.gov study NCT03356821. IPD Sharing: UNDECIDED. Countries: 1. Publications: 14.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.