Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
44
datasets available to search
ShareScore release 0.9.0
Dataset results
44 results for “Threonine”
X-ray diffraction images for L-threonine dehydrogenase from Trypanosoma brucei with NAD and pyruvate bound.
<p>X-ray diffraction images which were collected at ESRF (Grenoble) using an ADSC 315r CCD detector on beamline ID29 on 11th November 2009. More details are given in the uploaded notes. </p>
Diffraction images of a crystal of the F-BAR domain of PSTPIP1 (Proline-serine-threonine phosphatase-interacting protein 1) mutant G258A (PDB entry 7AAL)
<p>Diffraction images of a crystal of the F-BAR domain of PSTPIP1 (residues 1-289), mutant G258A.</p> <p>Data were collected on a single crystal at the beamline i03 of the Diamond Light Source synchrotron (Didcot, UK) using radiation of 0.9999 Å wavelength and a PILATUS3 6M detector. The dataset consists of 2400 images (0.15 degree oscillation per image). Crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions a=48.19 Å, b=73.02 Å, c=205.25 Å. The asymmetric unit contains an homodimer of the F-BAR domain (~53% solvent content), which is the biological unit.</p> <p>Diffraction data was notably anisotropic. The lowest resolution limit was 2.92 Å in the direction b* and the highest limits were 1.97 Å and 2.09 in the directions a* and c*, respectively.</p> <p> </p> <p>The structure derived form these data is published in:</p> <p>Manso, J.A., Marcos, T., Ruiz-Martín, V. Casas J, Alcón P, Sánchez Crespo M, Bayón Y, de Pereda JM, Alonso A <em>PSTPIP1-LYP phosphatase interaction: structural basis and implications for autoinflammatory disorders</em>. <strong>Cell. Mol. Life Sci</strong>. 79, 131 (2022). <a href="https://doi.org/10.1007/s00018-022-04173-w">https://doi.org/10.1007/s00018-022-04173-w</a></p> <p>The structure is available at the PDB under the code 7AAL:</p> <p><a href="https://www.ebi.ac.uk/pdbe/entry/pdb/7aal">https://www.ebi.ac.uk/pdbe/entry/pdb/7aal</a></p>
Diffraction images of a crystal of the F-BAR domain of PSTPIP1 (Proline-serine-threonine phosphatase-interacting protein 1) bound to the C-terminal homology (CTH) segment of the phosphatase LYP (PTPN22) (PDB entry 7AAM)
<p>Diffraction images of a crystal of the F-BAR domain of human PSTPIP1 (residues 1-289, Uniprot reference O43586-1) in complex with the CTH of LYP (residues 787-807, Uniprot Q9Y2R2-1).</p> <p>Data were collected on a single crystal at the beamline i03 of the Diamond Light Source synchrotron (Didcot, UK) using radiation of 0.99987 Å wavelength and a PILATUS3 6M detector. The dataset consists of 3 groups, each containing of 1800 images (0.1 degree oscillation per image), collected at three different positions of the same crystal. Crystal belongs to the space group P2(1)2(1)2(1) with unit cell dimensions a=48.0 Å, b=72.0 Å, c=205.0 Å. The asymmetric unit contains an homodimer of the F-BAR domain bound to a LYP-CTH (~53% solvent content), which is the biological complex.</p> <p>Diffraction data was notably anisotropic. The lowest resolution limit was 4.05 Å in the direction b* and the highest limits were 2.11 Å and 2.10 in the directions a* and c*, respectively.</p> <p> </p> <p>The structure derived form these data is published in:</p> <p>Manso, J.A., Marcos, T., Ruiz-Martín, V. Casas J, Alcón P, Sánchez Crespo M, Bayón Y, de Pereda JM, Alonso A <em>PSTPIP1-LYP phosphatase interaction: structural basis and implications for autoinflammatory disorders</em>. <strong>Cell. Mol. Life Sci</strong>. 79, 131 (2022). <a href="https://doi.org/10.1007/s00018-022-04173-w">https://doi.org/10.1007/s00018-022-04173-w</a></p> <p>The structure is available at the PDB under the code <strong>7AAM</strong>:</p> <p><a href="https://www.ebi.ac.uk/pdbe/entry/pdb/7aam">https://www.ebi.ac.uk/pdbe/entry/pdb/7aam</a></p>
Diffraction images of a crystal of the F-BAR domain of PSTPIP1 (Proline-serine-threonine phosphatase-interacting protein 1) (PDB entry 7AAN)
<p>Diffraction images of a crystal of the F-BAR domain of PSTPIP1 (residues 1-289).</p> <p>Data were collected on a single crystal at the beamline i03 of the Diamond Light Source synchrotron (Didcot, UK) using radiation of 0.99987 Å wavelength and a PILATUS3 6M detector. The dataset consists of 3600 images (0.15 degree oscillation per image) that were collected: 2400 at one position and the other 1200 at a second site in the same crystal. Crystal belongs to the space group P2(1)2(1)2(1) with unit cell dimensions a=48.3 Å, b=71.9 Å, c=204.6 Å. The asymmetric unit contains an homodimer of the F-BAR domain (~53% solvent content), which is the biological unit.</p> <p>Diffraction data was notably anisotropic. The lowest resolution limit was 4.32 Å in the direction b* and the highest limits were 2.12 Å and 2.17 in the directions a* and c*, respectively.</p> <p>The structure derived form these data is published in:</p> <p>Manso, J.A., Marcos, T., Ruiz-Martín, V. Casas J, Alcón P, Sánchez Crespo M, Bayón Y, de Pereda JM, Alonso A <em>PSTPIP1-LYP phosphatase interaction: structural basis and implications for autoinflammatory disorders</em>. <strong>Cell. Mol. Life Sci</strong>. 79, 131 (2022). <a href="https://doi.org/10.1007/s00018-022-04173-w">https://doi.org/10.1007/s00018-022-04173-w</a></p> <p>The structure is available at the PDB under the code <strong>7AAN</strong>:</p> <p><a href="https://www.ebi.ac.uk/pdbe/entry/pdb/7aan">https://www.ebi.ac.uk/pdbe/entry/pdb/7aan</a></p>
Trypanosoma brucei L-threonine dehydrogenase diffraction images.
<p>Diffraction images for apo-L-threonine dehydrogenase from <em>T. brucei</em>. </p>
Fig. 4 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 4. TT motif is not required for the production of ent-kaurene (1). Extracted ion (m/z = 272) chromatograms demonstrating the production of 1 by all mutants (as indicated) by comparison to wild-type (WT) AtKS.
Fig. 5 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 5. TT motif has minimal effect on catalytic efficiency. Curve fit of Michaelis-Menton equation to data for wild-type (WT) and indicated mutants of AtKS.
Fig. 3 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 3. Identification of the TT motif. A) Representative alignment of KSs spanning plant evolution with the KS specific pair of threonines (TT motif) indicated by asterisks (*) above the alignment. B) Sequence logos demonstrating the absolute conservation of this TT motif in KS (bottom) relative to the derived KS(L)/TPS-e subfamily more generally (top). C) Location of TT motif in active site from model of AtKS (blue) containing 2-fluoroGGPP (green) and Mg2+ co-factors (magenta) derived from the template co-crystal structure of taxadiene synthase (Koksal et al., 2011).
Fig. 2 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 2. Representative phylogenetic tree for plant KS(L)/TPS-e subfamily. Green lines indicate KS activity, red lines KSL that mediate alternative product outcome. Green text indicates KS with known or assumed role in phytohormone biosynthesis, while blue text indicates enzymes known to produce 1 for secondary metabolism – i.e., in maize (Fu et al., 2016).
Fig. 7 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 7. TT motif codons are not conserved. A) Exon sizes from KS with known genomic sequence. B) Sequence of TT motif containing exon from AtKS (codons for TT motif in larger blue text). C) Sequence logo indicating lack of conservation of the codons for the TT motif – i.e., in the variable third/'wobble' position.
Fig. 6 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 6. TT motif has minimal effect on protein structure. CD spectra for wildtype (WT) and the T527 V/T528V (TT/VV) double mutant of AtKS (note that the minimal differences observed here are not reproducible).
A Physiological Study to Determine the Enteral Threonine Requirements in Infants Aged 1 to 6 Months
ClinicalTrials.gov study NCT02364843. IPD Sharing: NO. Countries: 1. Publications: 14.
Length of Adaptation and Threonine Requirement
ClinicalTrials.gov study NCT04585087. IPD Sharing: NO. Countries: 1. Publications: 1.
Trypanosoma brucei L-threonine dehydrogenase high resolution diffraction images.
<p>Diffraction images obtained in the high resolution pass for apo-L-threonine dehydrogenase from <em>T. brucei</em>. </p>
Fig. 1 in A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis
Fig. 1. KS reaction mechanism.
Threonine-4 of the budding yeast RNAP II CTD couples transcription with Htz1-mediated chromatin remodeling
GEO Series GSE60181. Saccharomyces cerevisiae. 4 samples. Type: Expression profiling by high throughput sequencing.
Threonine Phosphorylation of STAT1 Restricts Interferon Signaling and Promotes Innate Inflammatory Responses
GEO Series GSE261824. Mus musculus. 16 samples. Type: Expression profiling by high throughput sequencing.
Bisulfite sequencing with Daphnia highlights a role for epigenetics in regulating stress response to Microcystis through preferential differential methylation of serine and threonine amino acidsing bi
GEO Series GSE83407. Daphnia magna. 6 samples. Type: Methylation profiling by high throughput sequencing.
Microarry and RNA-seq analysis of M. tuberculosis response to threonine auxotrophic starvation
GEO Series GSE119107. Mycobacterium tuberculosis H37Rv; Mycobacterium tuberculosis. 20 samples. Type: Expression profiling by array; Expression profiling by high throughput sequencing.
Whole genome ChIP-seq of histone H3 threonine 11 phosphorylation in Saccharomyces cerevisiae
GEO Series GSE100564. Saccharomyces cerevisiae. 10 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.