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132 results for “Tissue patterning”
Dataset for "Fast creation of data-driven low-order predictive cardiac tissue excitation models from recorded activation patterns"
<p>This archive contains the source code and data sets presented in the publication "Fast creation of data-driven low-order predictive cardiac tissue excitation models from recorded activation patterns".</p> <p>Kabus, D., De Coster, T., de Vries, A. A., Pijnappels, D. A., & Dierckx, H. (2024). Fast creation of data-driven low-order predictive cardiac tissue excitation models from recorded activation patterns. <em>Computers in Biology and Medicine</em>, 107949. <a title="Persistent link using digital object identifier" href="https://doi.org/10.1016/j.compbiomed.2024.107949" target="_blank" rel="noreferrer noopener"><span>https://doi.org/10.1016/j.compbiomed.2024.107949</span></a></p>
Raw data: Patterned apoptosis has an instructive role for local growth and tissue shape regulation in a fast-growing epithelium
<p>What regulates organ size and shape remains one fundamental mystery of modern biology. Research in this area has primarily focused on deciphering the regulation in time and space of growth and cell division, while the contribution of cell death has been overall neglected. This includes studies of the <i>Drosophila</i> wing, one of the best characterised systems for the study of growth and patterning, undergoing massive growth during larval stage and important morphogenetic remodeling during pupal stage. So far, it has been assumed that cell death was relatively neglectable in this tissue both during larval and pupal stage and as a result the pattern of growth was usually attributed to the distribution of cell division. Here, using systematic mapping and registration combined with quantitative assessment of clone size and disappearance as well as live imaging, we outline a persistent pattern of cell death and clone elimination emerging in the larval wing disc and persisting during pupal wing morphogenesis. Local variation of cell death is associated with local variation of clone size, pointing to an impact of cell death on local growth which is not fully compensated by proliferation. Using morphometric analyses of adult wing shape and genetic perturbations, we provide evidence that patterned death affects locally and globally adult wing shape and size. This study describes a roadmap for precise assessment of the contribution of cell death to tissue shape, and outlines an important instructive role of cell death in modulating quantitatively local growth and morphogenesis of a fast-growing tissue.</p><p>This repository contains the raw data (local projection images, ROI and quantificatiosn) of the Current Biology article "<strong>Patterned apoptosis has an instructive role for local growth and tissue shape regulation in a fast-growing epithelium".</strong></p>
Research data supporting "Engineering anisotropic muscle tissue using acoustic cell patterning"
<p>Raw research data supporting the publication:</p> <p>Armstron, JPK et al., "Engineering anisotropic muscle tissue using acoustic cell paterning", Advanced Materials, DOI: 10.1002/adma.201802649 (2018)</p>
Splicing patterns of the Arabidopsis organellar rhomboid At1g74140 change during development from young to mature leaf tissues.
<p><strong>Background to the study:</strong> Four distinct rhomboid genes appear to function in <em>Arabidopsis</em> plastids, two “active” types from the secretases and presenilin-like associated rhomboid-like (PARL) categories (At1g25290 and At5g25752) and two “inactive” rhomboid forms (At1g74130 and At1g74140). The number of working rhomboids is further increased by alternative splicing, two reported for At1g25290 and three for At1g74130. Since At1g25290 and At1g74130 exist as alternative splice variants, it would be necessary to assess the splicing patterns of the other two plastid rhomboid genes, At5g25752 and At1g74140, before studying the <em>Arabidopsis</em> plastid rhomboid system as a whole. </p> <p><strong>Method used:</strong> This study thus specifically focused on an analysis of the At1g74140 transcript population using various RT-PCR strategies. The supplementary table provides the details of the various RT-PCR primers used in this study.</p> <p><strong>Results of the study and their relationship to the supplementary figures deposited here:</strong> The exon mapping results indicate splicing patterns different from the close relative At1g74130, despite similarity between the exonic sequences. The splicing patterns indicate a high level of sequence “discontinuity” in the At1g74140 transcript population with a significant portion of the discontinuity being generated by two regions of the gene. The three supplementary figures of RT-PCR results deposited here provide further evidence that the alternatively spliced short transcript products from the discontinuous splicing patterns fluctuate during development.</p> <p><strong>Conclusion of the main study and its relationship to the supplementary results deposited here:</strong> The overall discontinuous splicing pattern of At1g74140 may be reflective of its mode of involvement in activities like controlling gene expression. The developmental evidence in these deposited supplementary RT-PCR results indicate that some of the alternatively spliced short transcript products fluctuate between mature and young tissues, suggesting that the short transcripts bear functional significance.</p>
Fig. 1. Abomasal tissue infected predominantly with A. sidemi. A in Nematode-induced pathological lesions and alterations of mucin pattern identified in abomasa of wild ruminants
Fig. 1. Abomasal tissue infected predominantly with A. sidemi. A. Fallow deer, positive for Ashworthius sidemi. Washed abomasal mucosa with apparent extensive areas of hyperemia (arrowheads) and hemorrhagic lesion in the area of the fundus (arrow). B. Red deer, sample from gross lesion. Part of the abomasum with dispersed round-cellular interstitial inflammation at the base of the glands (arrow) and between abomasal pits (arrowheads). Full findings are accompanied by interstitial edema (asterisk). Hematoxylin and eosin (H&E) staining, magnification ×200. C. Fallow deer, sample from gross lesion. Part of the abomasum with hyperemia, consisting of several groups of vital erythrocytes in the interstitial tissue (arrow), including edema (arrowheads). H&E staining, magnification ×200. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Text-fig. 5. Reyispermum parvum gen. et sp. nov. seeds from the Early Cretaceous Vale de Água locality, Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, volume renderings). a) Holotype (S174178; Vale de Agua sample 141) in lateral view showing shape and cell pattern; remains of mounting media (¤). b) Cut volume rendering of seed (cut at yz0553) showing the slightly raised tissue immediately adjacent to the lower edge of the hilum (arrow head) and palisade-shaped cells of exotesta. c) Apical view of seed showing hilar depression (hi), position of micropylar slit (mi) and the slightly raised raphal ridge (ra). d) Seed in lateral view showing raised tissue immediately adjacent to the lower edge of the hilum (arrow head) (S174495, Vale de Água sample 300). e) Cut volume rendering (cut at yz0500) of the seed in (5d) showing the raised tissue (arrow head) immediately adjacent to the lower edge of the hilum and sclerenchyma cells of exotesta. f) Detail of seed in (5d) showing micropylar slit (mi), hilum (hi) and raised tissue (arrow head) immediately adjacent to the lower edge of the hilum. g, h) Seed in lateral view (g) and view towards raphe (h) showing seed shape, the raised tissue below hilum (arrow head) and the raphal ridge (ra); note pointed micropylar area (S174179, Vale de Água sample 141). i) Seed surface of seed in (5d) showing the raised outlines of the undulate anticlinal walls of the exotestal cells. Scale bars = 250 µm (a–e, g, h); 125 µm (f, i). in Extinct Taxa Of Exotestal Seeds Close To Austrobaileyales And Nymphaeales From The Early Cretaceous Of Portugal
Text-fig. 5. Reyispermum parvum gen. et sp. nov. seeds from the Early Cretaceous Vale de Água locality, Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, volume renderings). a) Holotype (S174178; Vale de Agua sample 141) in lateral view showing shape and cell pattern; remains of mounting media (¤). b) Cut volume rendering of seed (cut at yz0553) showing the slightly raised tissue immediately adjacent to the lower edge of the hilum (arrow head) and palisade-shaped cells of exotesta. c) Apical view of seed showing hilar depression (hi), position of micropylar slit (mi) and the slightly raised raphal ridge (ra). d) Seed in lateral view showing raised tissue immediately adjacent to the lower edge of the hilum (arrow head) (S174495, Vale de Água sample 300). e) Cut volume rendering (cut at yz0500) of the seed in (5d) showing the raised tissue (arrow head) immediately adjacent to the lower edge of the hilum and sclerenchyma cells of exotesta. f) Detail of seed in (5d) showing micropylar slit (mi), hilum (hi) and raised tissue (arrow head) immediately adjacent to the lower edge of the hilum. g, h) Seed in lateral view (g) and view towards raphe (h) showing seed shape, the raised tissue below hilum (arrow head) and the raphal ridge (ra); note pointed micropylar area (S174179, Vale de Água sample 141). i) Seed surface of seed in (5d) showing the raised outlines of the undulate anticlinal walls of the exotestal cells. Scale bars = 250 µm (a–e, g, h); 125 µm (f, i).
Data for: Relatively rare root endophytic bacteria drive plant resource allocation patterns and tissue nutrient concentration in unpredictable ways
<p><span><span><span><span><span><span><span><span><span><span><span><b>Premise of Study</b></span></span></span></span></span></span></span></span></span></span></span></p> <p><span><span><span><span><span><span><span><span><span><span><span>Plant endophytic bacterial strains can influence plant traits such as leaf area and root length. Yet, the influence of more complex bacterial communities in regulating overall plant phenotype is less explored. Here, we conducted two complementary experiments to test if we can predict plant phenotype response to changes in microbial community composition. </span></span></span></span></span></span></span></span></span></span></span></p> <p><span><span><span><span><span><span><span><span><span><span><span><b>Methods</b></span></span></span></span></span></span></span></span></span></span></span></p> <p><span><span><span><span><span><span><span><span><span><span><span>In the first study, we inoculated a single genotype of <i>Populus deltoides</i>with individual root endophytic bacteria and measured plant phenotype. Next, single inoculation data were used to predict phenotypic traits in mixed three-member community inoculations, which we tested in the second experiment. </span></span></span></span></span></span></span></span></span></span></span></p> <p><span><span><span><span><span><span><span><span><span><span><span><b>Key Results</b></span></span></span></span></span></span></span></span></span></span></span></p> <p><span><span><span><span><span><span><span><span><span><span><span>When in isolation, each bacterial endophyte significantly but weakly altered plant phenotype relative to non-inoculated plants. In mixture, bacterial strain <i>Burkholderia</i>BT03, constituted at least 98% of community relative abundance. Yet, plant resource allocation and tissue nutrient concentrationswere disproportionately influenced by <i>Pseudomonas </i>sp.GM17, GM30, and GM41. We found a 10% increase in leaf mass fraction and a 11% decrease in root mass fraction when replacing<i>Pseudomonas </i>GM17 with GM41 in communities containing both <i>Pseudomonas </i>GM30 and <i>Burkholderia</i>BT03. </span></span></span></span></span></span></span></span></span></span></span></p> <p><span><span><span><span><span><span><span><span><span><span><span><b>Conclusions</b></span></span></span></span></span></span></span></span></span></span></span></p> <p><span><span><span><span><span><span><span><span><span><span><span>Our results indicate that interactions among endophytic bacteria may drive plant phenotype over the contribution of each strain individually. Additionally, we have shown that low-abundant strains contribute to plant phenotype challenging the assumption that the dominant strains will drive plant function.</span></span></span></span></span></span></span></span></span></span></span></p>
Additional data for "Tissue-specific impacts of aging and genetics on gene expression patterns in humans"
<p>Additional files for paper "Tissue-specific impacts of aging and genetics on gene expression patterns in humans" by Yamamoto and Chung et al. 2021.</p> <p>Contains file of results from joint age and genetics model.</p>
Transcriptome data from silica-preserved leaf tissue reveals gene flow patterns in a Caribbean bromeliad
<p>Transcriptome sequencing is a cost-effective approach that allows researchers to study a broad range of questions. However, to preserve RNA for transcriptome sequencing, tissue is often kept under special conditions, such as immediate ultracold freezing. Here, we demonstrate that RNA can be obtained from six-month-old, field-collected samples stored in silica gel at room temperature. Using these transcriptomes, we explore the evolutionary relationships of the genus Pitcairnia (Bromeliaceae) in the Dominican Republic and infer barriers to gene flow.</p>
Patterns of Care and Outcomes in METAstatic Soft Tissue SARComa
ClinicalTrials.gov study NCT03977818. IPD Sharing: NO. Countries: 1. Publications: 1.
Variation in the production of plant tissues bearing extrafloral nectaries explains temporal patterns of ant attendance in Amazonian understory plants
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Data for: Relatively rare root endophytic bacteria drive plant resource allocation patterns and tissue nutrient concentration in unpredictable ways
Open the record for dataset details and reuse information.
Root tissue carbon and nitrogen:Effect of Burning Patterns on Vegetation in the Fish Lake Burn Compartments
This study examines the effects of long-term prescribed burning treatments on vegetation structure and composition, productivity, and nutrient cycling in upland oak savanna and woodland vegetation. The basis for the study is an ongoing, experimental prescribed burning program begun in 1964 at Cedar Creek, and a similar program operating since 1962 on the adjacent Helen Allison Savanna property (owned by The Nature Conservancy). These prescribed burning programs are designed to subject upland oak communities (and some old fields) to different burn frequencies and patterns of burning, with the ultimate objectives of 1) restoring and maintaining the historically important savanna and open woodland vegetation, and 2) providing information about the effects of different burning patterns on vegetation structure and composition. This study addresses the latter of these two purposes and expands on it by also investigating possible influences of fire on resource availability (nutrients, water, and light) and net primary productivity. This study represents a continuation and expansion of experiments 015 and 094.
Data from: Seasonal patterns in δ2H values of multiple tissues from Andean birds provide insights into elevational migration
Elevational migration is a widespread phenomenon in tropical avifauna but it is difficult to identify using traditional approaches. Hydrogen isotope (δ2H) values of precipitation decrease with elevation so δ2H analysis of multiple bird tissues with different isotopic incorporation rates may be a reliable method for characterizing seasonal elevational migration. Here we compare δ2H values in metabolically inert (feathers and claws) and metabolically active (whole blood) tissues to examine whether an upslope migration occurs prior to the breeding season in the Yungas Manakin (Chiroxiphia boliviana). We compare results from C. boliviana with data from a known elevational migrant, the Streak-necked Flycatcher (Mionectes striaticollis). Opposite to our expectations, tissue δ2H values increased over time, largely reflecting seasonal patterns in precipitation δ2H rather than elevational effects; linear mixed-effects models with strongest support included ordinal date, tissue type and elevation. This seasonal increase in precipitation δ2H is a general phenomenon in both tropical and temperate mountain ranges. We use these data to propose a hypothetical framework that predicts different patterns in tissue δ2H values collected in different seasons from residents and elevational migrants. This framework can serve as a reference for future studies that assess elevational migration in birds and other animals.
Data from: Automated segmentation of complex patterns in biological tissues: lessons from stingray tessellated cartilage
Introduction - Many biological structures show recurring tiling patterns on one structural level or the other. Current image acquisition techniques are able to resolve those tiling patterns to allow quantitative analyses. The resulting image data, however, may contain an enormous number of elements. This renders manual image analysis infeasible, in particular when statistical analysis is to be conducted, requiring a larger number of image data to be analyzed. As a consequence, the analysis process needs to be automated to a large degree. In this paper, we describe a multi-step image segmentation pipeline for the automated segmentation of the calcified cartilage into individual tesserae from computed tomography images of skeletal elements of stingrays. Methods - Besides applying state-of-the-art algorithms like anisotropic diffusion smoothing, local thresholding for foreground segmentation, distance map calculation, and hierarchical watershed, we exploit a graph-based representation for fast correction of the segmentation. In addition, we propose a new distance map that is computed only in the plane that locally best approximates the calcified cartilage. This distance map drastically improves the separation of individual tesserae. We apply our segmentation pipeline to hyomandibulae from three individuals of the round stingray (Urobatis halleri), varying both in age and size. Results - Each of the hyomandibula datasets contains approximately 3000 tesserae. To evaluate the quality of the automated segmentation, four expert users manually generated ground truth segmentations of small parts of one hyomandibula. These ground truth segmentations allowed us to compare the segmentation quality w.r.t. individual tesserae. Additionally, to investigate the segmentation quality of whole skeletal elements, landmarks were manually placed on all tesserae and their positions were then compared to the segmented tesserae. With the proposed segmentation pipeline, we sped up the processing of a single skeletal element from days or weeks to a few hours.
Emergence of a geometric pattern of cell fates from tissue-scale mechanics in the Drosophila eye
<p>Pattern formation of biological structures involves the arrangement of different types of cells in an ordered spatial configuration. In this study, we investigate the mechanism of patterning the <em>Drosophila</em> eye into a precise triangular grid of photoreceptor clusters called ommatidia. Previous studies had led to a long-standing biochemical model whereby a reaction-diffusion process is templated by recently formed ommatidia to propagate a molecular prepattern across the eye epithelium. Here, we find that the templating mechanism is instead, mechanical in origin; newly born columns of ommatidia serve as a template to spatially pattern cell flows that move the cells in the epithelium into position to form each new column of ommatidia. Cell flow is generated by a pressure gradient that is caused by a narrow zone of cell dilation precisely positioned behind the growing wavefront of ommatidia. The newly formed lattice grid of ommatidia cells are immobile, deflecting and focusing the flow of other cells. Thus, the self-organization of a regular pattern of cell fates in an epithelium is mechanically driven.</p>
Spatial methods for fixed skin tissues reveal new molecular patterns and lncRNA
<p>Spatial transcriptomics (ST) to explore a vast amount of formalin-fixed paraffin-embedded (FFPE) archival cancer tissues has been highly challenging due to several critical technical issues. In this work, we optimised ST protocols to generate unprecedented spatial gene expression data for FFPE skin cancer. Skin is among the most challenging tissue types for ST due to its fibrous structure and a high risk of RNAse contamination. We evaluated tissues collected from ten years to two years ago, spanning a range of tissue qualities and complexity. Technical replicates and multiple patient samples were assessed. Further, we integrated gene expression profiles with pathological information, revealing a new layer of molecular information. Such integration is powerful in cancer research and clinical applications. The data allowed us to detect the spatial expression of non-coding RNAs. Together, this work provides important technical perspectives to enable the applications of ST on archived cancer tissues.</p>
Tissue-resident Memory T Cells Expression Among the Repigmentation Patterns Induced by NB-UVB Phototherapy in Vitiligo
ClinicalTrials.gov study NCT05506995. IPD Sharing: NO. Countries: 1. Publications: 10.
Data from: Euconodont hard tissue: preservation patterns of the basal body
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Data from: Seasonal patterns in δ2H values of multiple tissues from Andean birds provide insights into elevational migration
Open the record for dataset details and reuse information.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.