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7 results for “correction filters”
Inner filter effect correction for fluorescence measurements in microplates (ZINFE and NINFE) - experimental data
<p>Experimental data for the paper entitled <em>Inner Filter Effect Correction for Fluorescence Measurements in Microplates Using Variable Vertical Axis Focus</em> (https://doi.org/10.1021/acs.analchem.2c01031).</p> <p>Separate datasets are provided for data with background correction, without background correction, and absorbance-corrected data.</p> <p>All results were obtained using the online calculator service written in Javascript: https://ninfe.science (version 15.9.2021.).</p> <p>For additional details please visit: https://glymech.pharma.hr//GlyMech.html.</p> <p> </p>
Inner filter effect correction for fluorescence measurements in microplates (AddAbs) - experimental data
<p>Experimental data for the paper entitled <em>Reducing the Inner Filter Effect in Microplates by Increasing Absorbance? Linear Fluorescence in Highly Concentrated Fluorophore Solutions in the Presence of an Added Absorber</em> (<a href="https://doi.org/10.1021/acs.analchem.3c01295">https://doi.org/10.1021/acs.analchem.3c01295</a>).</p> <p>Separate worksheets are provided for the following:</p> <p>1. Fluorescence measurements - raw values in triplicate; 2 worksheets for transparent (T) and nontransparent (NT) microplates, each with 15 titrations: L<sub>1</sub>-L<sub>12</sub> and H<sub>1</sub>-H<sub>3</sub>,</p> <p>2. Absorbance measurements - raw values in triplicate (<em>λ</em><sub>ex</sub> = 345 nm, <em>λ</em><sub>em</sub> = 390 nm); 1 worksheet for T microplates only, with 4 titrations: L<sub>1</sub>-L<sub>4</sub>,</p> <p>3. Fluorescence measurements - averaged, baseline-corrected averaged and normalized baseline-corrected averaged triplicates; 2 worksheets for T and NT microplates, each with 15 titrations: L<sub>1</sub>-L<sub>12</sub> and H<sub>1</sub>-H<sub>3</sub>,</p> <p>4. ZINFE/NINFE-corrected fluorescence in T microplates; 12 worksheets for 12 titrations: L<sub>1</sub>-L<sub>9</sub> and H<sub>1</sub>-H<sub>3</sub>,</p> <p>5. ZINFE/NINFE-corrected fluorescence in NT microplates; 12 worksheets for 12 titrations: L<sub>1</sub>-L<sub>9</sub> and H<sub>1</sub>-H<sub>3</sub>.</p> <p>The results of the ZINFE and NINFE correction methods obtained using the online calculator service written in Javascript: https://ninfe.science (version 09.5.2022.)</p> <p>For additional details please visit: https://glymech.pharma.hr//GlyMech.html.</p>
Chromophore-driven optical path reduction as an indirect inner filter effect correction strategy in fluorescence microplate assays - experimental data
<p>Experimental data for the paper entitled <em>Chromophore-driven optical path reduction as an indirect inner filter effect mitigation strategy in fluorescence microplate assays</em> (<a title="Persistent link using digital object identifier" href="https://doi.org/10.1016/j.saa.2025.127049" target="_blank" rel="noreferrer noopener"><span><span>https://doi.org/10.1016/j.saa.2025.127049</span></span></a>). </p> <p>An Excel file with multiple worksheets is provided for the following:</p> <p>1. Fluorescence data:</p> <p>The worksheets contain fluorescence values measured in tetraplicates using two microplate readers (Tecan Spark M10, Tecan, Austria and SpectraMax iD3, Molecular Devices, USA), with baseline values recorded in octuplicates. The results were averaged and baseline-corrected. Detailed notes on the titration experiments are included in the manuscript. Data include both IFE-uncorrected fluorescence and fluorescence corrected (fully or partially) by the addition of an absorbing chromophore.</p> <p>2. Absorbance data:</p> <p>All measured absorbance values obtained using the Tecan Spark M10 microplate reader with UV-Vis transparent microplates are included. The absorbance values at specific wavelengths suitable for the Lakowicz IFE correction method or for determining the concentration of the compound are given separately.</p> <p>3. Fluorescein spectra:</p> <p>UV-Vis and fluorescence excitation/emission spectra for highly dilute fluorescein concentrations where the inner filter effect (IFE) is negligible are reported. These measurements were performed to obtain “true” excitation and emission spectra without the influence of IFE quenching effects.</p> <p>4. ZINFE/NINFE IFE corrections:</p> <p>The dataset contains the results of ZINFE/NINFE IFE corrections (<a href="https://doi.org/10.1021/acs.analchem.2c01031">https://doi.org/10.1021/acs.analchem.2c01031</a>) applied to the fluorescence data and processed using the web service available at <a href="https://ninfe.science">https://ninfe.science</a>.</p> <p>The notation of the fluorophores and microplates is the same as in the manuscript. For reasons of visual clarity, the numerical titration indices are not in subscript.</p> <p> </p>
Lorentzian filter correction of turbulence measurements on oscillating floating platforms: impact on wind spectra and eddy covariance fluxes
<p class="MsoTitle">Turbulence and eddy covariance measurements on a floating platform over water surfaces can be contaminated by platform oscillations, which may affect the calculated air–water exchange. The conventional method for decontamination of the platform oscillations from the wind velocity measurements requires the installation of an additional sensitive, and often costly, motion sensor. This paper examines a new mathematical decontamination method, termed Lorentzian filter, which avoids the need for such an instrument. The method, based on the Lorentzian function, capitalizes on the pseudo-harmonic behavior of the platform oscillations and reduces the amplitude of turbulent wind velocity data detected as artifacts at the specific natural frequencies of the platform. The Lorentzian filter was applied to wind velocity data measured by sonic anemometer and eddy covariance system over the Dead Sea, Israel, for 30 days. We examined three approaches of dealing with motion contamination: Lorentzian filter decontamination, motion sensor decontamination, and non-filtered raw wind velocity. Using the 3D wind velocity series, we examined the wind spectra, the co-spectra of water vapor concentration and horizontal wind speed with vertical wind speed and H2O and momentum fluxes. The Lorentzian filter performed very well in decontaminating the wind spectrum, meaning that it efficiently identified the contamination in the natural oscillation frequency and returned a decontaminated wind velocity time series. The co-spectra and fluxes were less prone to the contamination of platform oscillations, presumably due to low correlations between the spurious wind velocity components and other measured scalars, such as water vapor.</p>
Lorentzian filter correction of turbulence measurements on oscillating floating platforms: impact on wind spectra and eddy covariance fluxes
Open the record for dataset details and reuse information.
Heme_k2_Filtered bckg correct trans-norm
GEO Series GSE8744. Lactiplantibacillus plantarum; Lactiplantibacillus plantarum WCFS1. 4 samples. Type: Expression profiling by array.
A workflow and digital filters for correcting speed and equalisation errors on digitised audio open-reel magnetic tapes - Audio Samples
<p>This repository makes available the audio samples related to the paper:</p> <p>Niccolò Pretto, Nadir Dalla Pozza, Alberto Padoan, Anthony Chmiel, Kurt James Werner, Alessandra Micalizzi, Emery Schubert, Antonio Rodà, Simone Milani and Sergio Canazza, <em>A workflow and digital filters for compensating speed and equalisation errors on digitised audio open-reel magnetic tapes</em>, Journal of the Audio Engineering Society, Special Issue on Audio Filter Design, 2022.</p> <p>The experiment and the three case studies are described in the publication above.</p> <p>This repository contains two main directories (<strong>bold</strong> indicates directory names):</p> <ul> <li> <p><strong>Experiment Samples</strong>: the 10 seconds long samples used in the experiment;</p> </li> <li> <p><strong>Long Samples</strong>: the original 6 minutes long samples, one for each of the identified cases.</p> </li> </ul> <p>Here is the notation of the file naming:</p> <ul> <li> <p>W: recording (writing);</p> </li> <li> <p>R: reproducing;</p> </li> <li> <p>3: 3.75 NAB;</p> </li> <li> <p>7N: 7.5 NAB;</p> </li> <li> <p>7C: 7.5 CCIR;</p> </li> <li> <p>15C: 15 CCIR.</p> </li> </ul> <p>For what concerns the <strong>Experiment Samples</strong> directory, here is the summary of the recording/reproducing standards of the adopted samples and their notation:</p> <ul> <li> <p>SET A: Recording 3.75 NAB (W3) - Reproducing 7.5 CCIR (R7C);</p> </li> <li> <p>SET B: Recording 3.75 NAB (W3) - Reproducing 15 CCIR (R15C);</p> </li> <li> <p>SET C: Recording 7.5 NAB (W7N) - Reproducing 15 CCIR (R15C).</p> </li> </ul> <p>Here are the variants of the samples:</p> <ul> <li> <p>REFERENCE: produced by using the correct equalization standard;</p> </li> <li> <p>ANCHOR: the "Reference" altered with a low-pass filter, with pass band set at 7 kHz for music and 3.5 kHz for speech;</p> </li> <li> <p>INCORRECT: produced by using an intentionally incorrect equalization, created by mismatching the recording and reading curves and resampled to the correct speed;</p> </li> <li> <p>MATLAB: the “Incorrect” variant corrected by means of a Matlab script;</p> </li> <li> <p>API: the “Incorrect” variant corrected by means of an <em>ad hoc</em> web interface adopting Web Audio API, for simulating real-time correction in web applications.</p> </li> </ul> <p>Here is the samples list:</p> <ul> <li> <p>SET A:</p> <ul> <li> <p>Training:</p> <ul> <li> <p>sample4: Richard Wagner - <em>Ride of the Valkyries</em>;</p> </li> </ul> </li> <li> <p>Test:</p> <ul> <li> <p>sample1: Taylor Swift - <em>Shake It Off</em>;</p> </li> <li> <p>sample5: Queen - <em>We Will Rock You</em>;</p> </li> <li> <p>sample8: Bruno Maderna - <em>Continuo</em>;</p> </li> <li> <p>sample9: Luciano Berio - <em>Différences</em>.</p> </li> </ul> </li> </ul> </li> <li> <p>SET B (the track title reflects the name of the file from which the track itself was extracted, from the CLIPS project of the University of Napoli: <a href="http://www.clips.unina.it/en/index.jsp">http://www.clips.unina.it/en/index.jsp</a>):</p> <ul> <li> <p>Training:</p> <ul> <li> <p>sample22: CLIPS project - <em>LP4m18bZ</em>;</p> </li> </ul> </li> <li> <p>Test:</p> <ul> <li> <p>sample15: CLIPS project - <em>LP1f20bZ</em>;</p> </li> <li> <p>sample16: CLIPS project - <em>LP4m20bZ</em>;</p> </li> <li> <p>sample17: CLIPS project - <em>LP1f19bZ</em>;</p> </li> <li> <p>sample18: CLIPS project - <em>LP4m19bZ</em>.</p> </li> </ul> </li> </ul> </li> <li> <p>SET C:</p> <ul> <li> <p>Training:</p> <ul> <li> <p>sample3: Carl Orff - <em>Carmina Burana - O Fortuna</em>;</p> </li> </ul> </li> <li> <p>Test:</p> <ul> <li> <p>sample2: The Weeknd - <em>Save Your Tears</em>;</p> </li> <li> <p>sample6: Eagles - <em>Hotel California</em>;</p> </li> <li> <p>sample10: Bruno Maderna - <em>Musica su Due Dimensioni</em>;</p> </li> <li> <p>sample12: Bruno Maderna - <em>Syntaxis</em>.</p> </li> </ul> </li> </ul> </li> </ul>
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