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ShareScore release 0.9.0
Dataset results
36 results for “gRNA”
Joint AstraZeneca-Cancer Research Horizons Functional Genomics Centre's CRISPRn library benchmark screens: gRNA counts and associated metadata
<p>Genome-wide CRISPR sgRNA libraries have emerged as transformative tools to systematically probe gene function. While these libraries have been iterated over time to be more efficient, their large size limits their use in some applications. Here, we benchmarked publicly available genome-wide single-targeting sgRNA libraries and evaluated dual targeting as a strategy for pooled CRISPR loss-of-function screens. We leveraged this data to design two minimal genome-wide human CRISPR-Cas9 libraries that are 50% smaller than other libraries and that preserve specificity and sensitivity, thus enabling broader deployment at scale. </p>
Cross-species gRNA-design
<p>This dataset contains all scripts and data files required to run the <em><strong>Cross-species gRNA design </strong></em>shiny<em><strong> </strong></em>app created for the Perturb-seq project of the Hellmmann-Enard Lab. As part of this project, we plan to perturb a selection of transcription factors (TFs) using single-cell CRISPRi screens in primate iPS cells, infer gene regulatory networks (GRNs) based on the outcome of the perturbations, then quantitatively compare these GRNs aross species. </p> <p>During the experimental design, we selected TFs based on previous data that might be interesting to perturb and designed single-guide RNAs (gRNAs) to target them in the human and cynomolgus macaque genomes. The app aims to present all information about the TF selection and gRNA design in a structured and interactive manner.</p>
NGS Data from: Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage
<p><span><span>We engineered gRNAs with highly stable hairpins in their constant parts and further enhanced their stability by chemical modifications. The 'Genome-editing Optimized Locked Design' (GOLD)-gRNA increases genome editing efficiency up to around 1000-fold (from 0.08% to 80.5%) with a mean increase across different other targets of 7.4-fold. The related NGS data is deposited </span></span>here.</p>
NGS Data from: Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage
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GiRAFR improves gRNA detection and annotation in single cell CRISPR screens
GEO Series GSE216040. Homo sapiens. 2 samples. Type: Other.
gRNA-seq from Ishikawa after medroxyprogesterone treatment
GEO Series GSE241070. Homo sapiens. 3 samples. Type: Other.
Characterization of gRNA-Dependent and gRNA-Independent Off-Target Binding Sites of PspCas13b and RfxCas13d in Mammalian Cells [CLIP-seq]
GEO Series GSE291436. Homo sapiens. 47 samples. Type: Other.
ARID1A and the BAF complex are determinants of breast cancer treatment response [CRISPR gRNA-seq]
GEO Series GSE123283. Homo sapiens. 35 samples. Type: Other.
Characterization of gRNA-Dependent and gRNA-Independent Off-Target Binding Sites of PspCas13b and RfxCas13d in Mammalian Cells [RNA-Seq]
GEO Series GSE291437. Homo sapiens. 66 samples. Type: Expression profiling by high throughput sequencing.
Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency and patterns
GEO Series GSE127752. Mus musculus. 49 samples. Type: Expression profiling by high throughput sequencing; Other; Third-party reanalysis.
mRNA profiling on GM-CSF knockout CART19 compared to wildtype CART19 and Ctrl gRNA CART19 produced from normal donors
GEO Series GSE147991. Homo sapiens. 9 samples. Type: Expression profiling by high throughput sequencing.
Forward genetic screening in engineered colorectal cancer organoids identifies novel regulators of metastasis [crispr gRNA knockout screening 3]
GEO Series GSE263582. Mus musculus. 15 samples. Type: Other.
Forward genetic screening in engineered colorectal cancer organoids identifies novel regulators of metastasis [crispr gRNA knockout screening 2]
GEO Series GSE263581. Mus musculus. 30 samples. Type: Other.
Cas9 gRNA engineering for selectable genome editing, activation and repression
GEO Series GSE70694. Homo sapiens. 24 samples. Type: Expression profiling by high throughput sequencing.
IDLV capture for gRNA off-target analysis
GEO Series GSE133861. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
RNASeq of Arg2 gRNA or scrambled gRNA CRISPR'd Tregs
GEO Series GSE140031. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Combined CRISPR and proteomics screening reveal a cohesin-CTCF-bound allele contributing to increased expression of RUVBL1 and prostate cancer progression [gRNA]
GEO Series GSE224653. Homo sapiens; Expression vector lentiGuide-Puro. 10 samples. Type: Other.
Light induced expression of gRNA allows for optogenetic gene editing of T lymphocytes in vivo
GEO Series GSE275757. Homo sapiens. 8 samples. Type: Other.
Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity with CHANGE-seq-BE [gRNA sequencing for ABE targets]
GEO Series GSE311547. synthetic construct. 18 samples. Type: Other.
WT vs MO-grnA injected: 16, 24, 48, 72 hpf trunk region of zebrafish embryos
GEO Series GSE38441. Danio rerio. 12 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.