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8 results for “microfluidic channels”
Supplementary Table S1 (raw data) of "Filtration extraction method using microfluidic channel for measuring environmental DNA "
<p>Supplementary Table S1 (all raw data) of "Filtration extraction method using microfluidic channel for measuring environmental DNA ". Each data of the validation experiment; Experiment 1-4 was located in different sheets..</p>
injection of PVA MBs via microcatheter in microfluid channel
<p>jinjection tests operating the syringe manually, also in reverse flux, to verify the absence of obstructions and damages.Thereafter, the injection syringe was mounted on a programmable, step-by-step motor driven syringe pump system which was set at different constant volumetric flow rates, ranging from 0.05 ml/min to 1.12 ml/min according to material and methods, section "<em>Handling of microcatheters with PVA MBs for insertion in microfluidic channels"</em></p>
Node-Pore Coded Coincidence Correcting Microfluidic Channel Framework: Code Design and Sparse Deconvolution
<p>This is the dataset for the work titled and authored by:</p> <p><strong>Node-Pore Coded Coincidence Correcting Microfluidic Channel Framework: Code Design and Sparse Deconvolution</strong></p> <p>Michael Kellman, Francois Rivest, Alina Pechacek, Lydia Sohn, Michael Lustig</p> <p>We present a novel method to perform individual particle (e.g. cells or viruses) coincidence correction through joint channel design and algorithmic methods. Inspired by multiple-user communication theory, we modulate the channel response, with Node-Pore Sensing, to give each particle a binary Barker code signature. When processed with our modified successive interference cancellation method, this signature enables both the separation of coincidence particles and a high sensitivity to small particles. We identify several sources of modeling error and mitigate most effects using a data-driven self-calibration step and robust regression. Additionally, we provide simulation analysis to highlight our robustness, as well as our limitations, to these sources of stochastic system model error. Finally, we conduct experimental validation of our techniques using several encoded devices to screen a heterogeneous sample of several size particles.</p> <p>Software can be found under this DOI:</p> <p>10.5281/zenodo.846448</p>
Comparison of measurement protocols for internal channels of transparent microfluidic devices - datasets
<p>Supplementary material to "Comparison of measurement protocols for internal channels of transparent microfluidic devices" submitted to Micromachines.</p> <p>Data accompanying comparison of measurement protocols.</p> <p> </p> <p>Further readings can be found at <a href="https://mfmet.eu/publications">https://mfmet.eu/publications</a></p> <p>The project (20NRM02 MFMET) have received funding from the EMPIR programme co-financed by the Participating States and from the European Union’s Horizon 2020 research and innovation programme.</p>
Rapid Identification of Bacterial isolates Using Microfluidic Adaptive Channels and Multiplexed Fluorescence Microscopy
<p>Dataset for: Rapid Identification of Bacterial isolates Using Microfluidic Adaptive Channels and Multiplexed Fluorescence Microscopy</p> <p>doi: <a title="Link to landing page via DOI" href="https://doi.org/10.1039/D4LC00325J">10.1039/D4LC00325J</a></p>
Optical trapping of micro-particles and bacterial cells in single channel and flow-focusing microfluidic devices
<p><strong>Video 1</strong> - The video shows the flow-focusing and trapping of 1.84 μm bacteria-sized particles flowing at a sample flow rate of 0.1 μL/min. The horizontal sheath flow rate 1 μL/min and the vertical sheath flow rate is 0.5 μL/min. Trapping is achieved using a maximum laser power of 250mW. </p> <p><strong>Video 2</strong> - The video shows the flow and fluorescence trapping of 1.84 μm bacteria-sized particles flowing at a flow rate of 0.013 μL/min. The channel surface is treated with pluronic F-127 to prevent cell adhesion. </p> <p><strong>Video 3</strong> - The video shows the flow and trapping of 1.84 μm bacteria-sized particles flowing at a flow rate of 1 μL/min. Increased flow rate results in continuous transient trapping of the cells is achieved at a trapping power of 250mW. The microchannel surface is not treated with pluronic F-127, therefore lot of particles stick to the channel surface. </p> <p><strong>Video 4</strong> - The video shows the flow and trapping of 1.84 μm bacteria-sized particles flowing at a flow rate of 0.013 μL/min. Trapping is achieved at a laser power of 250mW. The channel surface is treated with pluronic F-127 to prevent cell adhesion. </p> <p><strong>Video 5</strong> - The video shows the flow and trapping of <em>E. coli</em> MG1655 flowing at a flow rate of 0.013 μL/min. Trapping is achieved at a laser power of 250mW. The channel surface is treated with pluronic F-127 to prevent cell adhesion. </p> <p><strong>Video 6</strong> - The video shows the flow and trapping of <em>S. aureus</em> 6538 flowing at a flow rate of 0.013 μL/min. Trapping is achieved at a laser power of 250mW. The channel surface is treated with pluronic F-127 to prevent cell adhesion. </p>
A novel approach to low-cost, rapid and simultaneous colorimetric detection of multiple analytes using 3D printed microfluidic channels
<p>This research paper presents an inventive technique to swiftly create microfluidic channels on distinct membrane papers, enabling colorimetric drug detection. Using a modified DIY RepRap 3D printer with a syringe pump, microfluidic channels (µPADs) are crafted on a flexible nylon-based substrate. This allows simultaneous detection of four common drugs with a single reagent. An optimized blend of Polydimethylsiloxane (PDMS) dissolved in hexane is used to create hydrophobic channels on various filter papers. The PDMS-hexane mixture infiltrates the paper's pores, forming hydrophobic barriers that confine liquids within the channels. These barriers are cured on the printer's hot plate, controlling channel width and preventing spreading. Capillary action drives fluid along these paths without spreading. This novel approach provides a versatile solution for rapid microfluidic channel creation on membrane papers. The DIY RepRap 3D printer integration offers precise control and faster curing. The PDMS-hexane solution accurately forms hydrophobic barriers, containing liquids within desired channels. The resulting microfluidic system holds potential for portable, cost-effective drug detection and various sensing applications.</p>
A novel approach to low-cost, rapid and simultaneous colorimetric detection of multiple analytes using 3D printed microfluidic channels
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