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Figures 28-40 in Oogenesis in Phragmatopoma (Polychaeta: Sabellariidae): Evidence for morphological distinction among geographically remote populations

Figures 28-40. Ultrastructure in Phragmatopoma caudata of the SW Atlantic. Growth Phase. Figure 28 Oocyte during late vitellogenesis filled with yolk granules (Y) and lipid droplets (Li). Note the cytoplasmic patches where the annulate lamellae (Al) are located and the welldeveloped egg envelope (Eg) (810x). Figure 29 Ripe oocytes occupy the entire coelomic cavity toward the body wall (810x). MC = muscle; Ep = epidermis. Figure 30 General view of the annulate lamellae (Al) between yolk granules and lipid droplets (560X). Figures 31-33 The annulate lamellae are composed by fenestrated endomembranes (arrows) parallel to each other arranged (Figure 31, 2,900X. Figure 32, 10,500X. Figure 33, 3,100X). Figure 34 Mature yolk granules with an elliptical shape (Y) showing a medullar crystalline substructure (white arrow) and non-crystalline cortex with electron-lucent spheres (black arrow) (13500X). Figure 35 Crystallised medullar in a parallel arrangement (white arrow) and a cortical lucid sphere (black arrow) (43,000X). Figure 36 Yolk granules (Y) and lipid droplets (Li) in the cytoplasm, with the presence of glycogen granules (arrow) close to the droplets (13,500X). M = mitochondria. Figure 37 Details of yolk granules delimited by the membrane (arrow) next to a lipid droplet (Li) and glycogen (Gly) (61000X). Figure 38 Cortical cytoplasm filled with yolk granules (Y), lipid droplets (Li) and cortical granules (CG) below the plasma membrane. Notice the thick egg envelope (Eg) (2,050X). Figure 39 The perivitelline space (1) is narrow and the egg envelope (Eg) is composed of two layers (2 and 3) with different electron-densities. The winding microvilli (Mv) are completely immersed in the egg envelope. Only the apical surface of the microvilli is in contact with the coelomic cavity (arrow) (5,400X). CG = cortical granules. Figure 40 Microvilli apex showing the expansion bears extensive filamentous adornment with an electron-dense centre (31,000X).

Figures 22-27 in Oogenesis in Phragmatopoma (Polychaeta: Sabellariidae): Evidence for morphological distinction among geographically remote populations

Figures 22-27. Ultrastructure in Phragmatopoma caudata of the SW Atlantic. Grow Phase. Figure 22 Oocyte during early vitellogenesis (Ev) with attributes of several yolk granules (Y) and lipid droplets (Li) in the cytoplasm, adjacent to an oocyte in late vitellogenesis (Lv) which vitelline envelope (Eg) thick (1,450X). N = nucleus, arrow = clumps of heterochromatin. Figure 23 Nucleus showing the heterochromatin clumps (large white arrow) and many complex pores in the nuclear envelope (small white arrows). The perinuclear cytoplasm aspect of granular material accumulations, juxtaposed with the nuclear envelope (black arrow) and next to mitochondria (M) (2,050X). Figure 24 Small and rounded yolk granules (Y) with different electron-densities and lucid spheres are observed inside. Besides Vesicles containing some electron-dense material (white arrow) also have lucid spheres (black arrow) and resemble nascent yolk granules (8,200X). Figure 25 Yolk granules (Y) of oocytes at the beginning of vitellogenesis showing varied electron-densities (black arrow) and electron-lucent spheres (large white arrow). Glycogen is also noticed (small white arrow) (21,500X). Figure 26 Cortical cytoplasm showing Golgi complexes (GC) close to the cortical granules (CG), which are filled with a fibrous material (black arrow). Note the large amount of glycogen (Gly) in the cytoplasm (1,150X). White arrow = glycogen. Figure 27 Microvilli (Mv) are larger and the egg envelope (Eg) thicker (2), relative to the previous stage. The perivitelline space (1) appears thinner (1500X).

Figures 15-21 in Oogenesis in Phragmatopoma (Polychaeta: Sabellariidae): Evidence for morphological distinction among geographically remote populations

Figures 15-21. Ultrastructure in Phragmatopoma caudata of the SW Atlantic. Grow Phase. Figures 15 and 16 Previtellogenic oocyte with a large nucleus (N) and nucleolus (Nu), as well as small rough heterochromatin clumps (small arrow). The cytoplasm is filled with rough, lamellar endoplasmic reticulum (RER) and noticeable mitochondria (M) in the perinuclear cytoplasm. The elongated portions of these cells create adhesion with adjacent oocyte (large arrow) (Figure 15, 2,050X. Figure 16, 4,200X). Oc = oocyte. Figure 17 Cytoplasm showing glycogen α (Gly) greater than the ribosomes (white arrows) attached to the reticulum (black arrow) (13,500X). M = mitochondria. Figures 18 and 19 Cortical cytoplasm of the rounded surface, showing Golgi complexes (GC) close to the cortical granules (CG), positioned beneath the microvilli (Mv) with the expansion bearing extensive filamentous adornment above the vitelline envelope (Eg) (Figure 18, 10,500x. Figure 19, 5,400X). Figures 20 and 21 Plasma membrane on a rounded surface showing endocytic depressions (black arrow) and coated vesicles (Cv). The vitelline envelope is composed by means of medium-apical extracellular matrix (2) in relation to microvilli (Mv). The basal region is electron-lucent and forming the beginnings of the perivitelline space (1) (Figure 20. 13,500X. Figure 21, 28,000X). GC = Golgi complexes, Eg = vitelline envelope.

Figures 10–14 in Oogenesis in Phragmatopoma (Polychaeta: Sabellariidae): Evidence for morphological distinction among geographically remote populations

Figures 10–14. Ultrastructure in Phragmatopoma caudata of the SW Atlantic. Proliferative Phase. Figures 10 and 11 Oogonia (Oo) and oocytes (Oc) connected via cellular prolongations (white arrow) to the endothelium of the intersegmental blood vessel (Bv). Note that the contact of ovary basal lamina is quite electron dense (black arrow). Desmosomes (D) form the oocyte-oocyte junction (Figure 10, 1,150X; Figure 11, 2,050X). M = mitochondria; Ch = chromosome. Figure 12 Oogonia with mitotic chromosomes (Ch) and oocytes in prophase with finely granular chromatin and chromosomes united by synaptonemal complexes (arrows) (2,400X). Figure 13 Synaptonemal complex (33,600X). N = nucleus. Figure 14 Narrow cytoplasm in oogonia (Oo) androunded nucleus. Oocyte (Oc) with wide cytoplasm and adhesion via desmosomes (D) (2,050X).

Figures 1–9 in Oogenesis in Phragmatopoma (Polychaeta: Sabellariidae): Evidence for morphological distinction among geographically remote populations

Figures 1–9. Histology and Histochemistry in Phragmatopoma caudata of the SW Atlantic. Figures 1 and 2 Oogonia (Oo) and previtellogenic oocytes (Pv) of early vitellogenesis (Ev) associated with the intersegmental blood vessels (BV) for cytoplasmic prolongations (black star). Oocytes in advanced and late vitellogenesis are released into the coelomic cavity (white star). Oocytes in vitellogenesis have intensely acidophilic granules, and the ripe oocytes are less acidophilus (black arrows). Figure 2 depicts oocytes in late vitellogenesis occupying the entire coelom with a basophils vitelline envelope (white arrow). N = nucleus; H&E staining. Scales = 20 μm. Figures 3 and 4 Techniques used to visualize basic and total proteins, respectively. The previtellogenic and vitellogenesis (Ev) oocytes (Pv) have intense granules (large black arrow), and the ripe in late vitellogenesis (Lv) are less reactive (white arrow). The vitelline envelope is reactive to protein (small arrow). Scales = 20 μm. Figure 5 Technique for visualizing neutral polysaccharides with positive staining in both the previtellogenic oocyte cytoplasm and the vitellogenic oocyte granules. The reactivity disappears in the vitellogenic granules of ripe oocytes, but surrounding these is a noticeable positive staining (large black arrow). Star = oogonia with little reactivity to neutral polysaccharides. The egg envelope is reactive to PAS (small arrow). Scale = 20 μm. Figures 6 and 7 Absence of acid polysaccharides (pH 1.0 and 2.5), respectively, in oogonia and oocytes in P. caudata. Scales = 20 μm. Figures 8 and 9 Lipids stained by Sudan Black B. The oogonia are uniformly positive (small white arrow), while the oocytes during previtellogenesis and vitellogenesis have droplets in the cytoplasm (large black arrow). The yolk granules are reactive during vitellogenesis, while the staining is less intensive (large white arrows) in the mature oocytes. The vitelline envelopes have lipids (small black arrow). Scales = 20 μm.

opencc-by-4.0Oct 2024View details →

Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster

#Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster ##Authors Abhishek Meena, Alessio N. De Nardo, Komal Maggu, Sonja Sbilordo, Benjamin Eggs, Rawaa Al Toma Sho, Stefan Lüpold ## Citation of associated article Meena, A., Maggu, K., De Nardo, A.N., Sbilordo, S.H., Eggs, B., Al Toma Sho, R., Lüpold, S., 2024. Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster. Journal of Thermal Biology 125, 104001. https://doi.org/10.1016/j.jtherbio.2024.104001   ##Overview This dataset was collected to assess the impact of heat stress across various life stages on reproductive performance in Drosophila melanogaster.  The study focuses on stage- and sex-specific reproductive metrics such as: mating success, fertility, fecundity, hatching success, which for females were summed over four days and for males were assayed for four consecutive 24-hour periods, each with a different female.  The dataset is structured to analyze these outcomes at different temperatures and developmental stages, providing insights into sex- and stage-specific vulnerabilities to heat stress.  The analyses are performed in an R Markdown (.Rmd) file, using data stored in a CSV file ##Contents The dataset includes the following files: Rscript.Rmd: An R Markdown file containing code for analyzing sex- and stage-specific reproductive fitness based on the data in Data.csv. Data.csv: A CSV file with the raw data used for the analysis. ##Data Description Note: NA's in each data file represent missing data (data not available).  The Data.csv file contains the following columns: Unique.ID: Unique identifier for each experimental individual. SampleID: Identifier for each treatment group. Temperature: Thermal exposure temperature (measured in degrees Celsius) for 4 hours, with levels: 24.5°C, 28°C, 32°C, 36°C, and 38°C. Lifestage: Developmental stage during which thermal treatment was applied. Sex: Sex of the individual exposed to the thermal treatment. Day: Days post adult treatment exposure. Block: Experimental block identifier for randomized grouping. Total: Total number of eggs laid within 24 hours of oviposition. Hatched: Number of eggs that successfully hatched. Unhatched: Number of eggs that did not hatch (derived from the difference between Total and Hatched). ##Usage To analyze the dataset, open and run the respective R Markdown (Rmd) files in RStudio or any compatible R Markdown environment. The Rmd files contain all the necessary code to reproduce the analyses described in the overview.

Figs 7–8 in Flight muscles degeneration, oogenesis and fat body in Lasius niger and Formica rufa queens (Hymenoptera: Formicidae)

Figs 7–8. Indirect flight muscles, gaster fat body and ovaries in four young queens of F. rufa: 7 — from left to right: dissected mesosoma; gaster without tergites; gaster without part of the fat body for two alates; 8 — the same anatomy as described in 7 for two single dealate queens; abbreviations in the text. Scales: 1 mm. Рис. 7–8. Крыловые мыШцы непрЯмого действиЯ, жировое тело и Яичники четырёх молодых самок F. rufa: 7 — слева-направо: вскрытаЯ меЗосома; брюШко беЗ тергитов; брюШком с удаленной частью жирового тела у двух крылатых самок, 8 — то же, у двух бескрылых одиночных самок; обоЗначениЯ в тексте. МасШтаб — 1 мм.

Fig. 3 in Flight muscles degeneration, oogenesis and fat body in Lasius niger and Formica rufa queens (Hymenoptera: Formicidae)

Fig. 3. Stages of indirect flight muscles degeneration in Formica rufa queens. Lateral view of a dissected mesosoma. Arrows indicate transitions from stages without external signs of necrosis (0) to stages in which hystolysis of muscle fibers occur (Hi1, Hi2) and their replacement by columns of adipocytes (Ad1, Ad2); other abbreviations — in the text. Рис. 3. Стадии дегенерации крыловых мыШц непрЯмого действиЯ у самок F. rufa. ВскрытаЯ меЗосома, сбоку. Стрелками покаЗаны переходы от стадии беЗ проЯвлений некроЗа (0) до гистолиЗа мыШечных волокон (Hi1, Hi2) и их ЗамеЩениЯ колонками адипоцитов (Ad1, Ad2); остальные обоЗначениЯ — в тексте.

Figs 4–6 in Flight muscles degeneration, oogenesis and fat body in Lasius niger and Formica rufa queens (Hymenoptera: Formicidae)

Figs 4–6. Indirect flight muscles, gaster fat body and ovaries in three young dealate queens of L. niger: 4–5 — from left to right, a dissected mesosoma from a lateral view, a dorsal view of gaster without tergites, gaster without part of fat body; 6 — from left to right, a dissected mesosoma from a lateral view, ovaries from dorsal and ventral view; abbreviation in the text. Scales: 1 mm. Рис. 4–6. Крыловые мыШцы непрЯмого действиЯ, жировое тело брюШка и Яичники трёх молодых бескрылых самок L. niger: 4– 5 — слева-направо, вскрытаЯ меЗосома, сбоку; вид на брюШко беЗ тергитов, сверху; то же — удалена часть жирового тела; 6 — слева- направо, вскрытаЯ меЗосома, сбоку; Яичники — сверху и сниЗу; обоЗначениЯ в тексте. МасШтаб — 1 мм.

Figs 1–2 in Flight muscles degeneration, oogenesis and fat body in Lasius niger and Formica rufa queens (Hymenoptera: Formicidae)

Figs 1–2. Reproductive systems in queen and workers of red wood ants: 1 — polytrophic ovariole, ovaries and oviducts in queen [partly after Schwanwitsch, 1949 and Otto, 1962]; 2 — stages of ovarian development in workers of F. rufa [after Otto,1958]; J — initial stage of ovarie, E1–E3 and V — growth stages, R1, R2 — stages of resorption, D — stage of degeneration; other — in the text. Рис. 1–2. ПоловаЯ система самки и рабочих у рыжих лесных муравьёв: 1 — политрофическаЯ ЯйцеваЯ трубочка, Яичники и половые пути самки [частично по Schwanwitsch, 1949 и Otto, 1962]; 2 — стадии раЗвитиЯ Яичников у рабочих F. rufa [по Otto,1958]; J — начальнаЯ стадиЯ Яичника, E1–E3 и V — стадии роста Яичников, R1, R2 — стадии реЗобциии, D — стадии дегенерации; прочeе — в тексте

Figs 9–12. A in Flight muscles degeneration, oogenesis and fat body in Lasius niger and Formica rufa queens (Hymenoptera: Formicidae)

Figs 9–12. A dissected mesosoma, gaster fat body and ovaries in familial dealate queens of F. rufa at different stages of indirect flight muscle degeneration are shown:9 — at the stage of progressive hystolysis, Hi1; 10 — at the stage of active hystolysis, Hi2; 11 — at the stage of progressive adipogenesis, Ad1; 12 — at the stage of completed adipogenesis, Ad2; abbreviations uin the text. Scale — 1 mm Рис. 9–12. МеЗосома, жировое тело брюШка и Яичники четырех семейных самок F. rufa на раЗных стадиЯх дегенерации крыловой мускулатуры: 9 — на стадии продвинутого гистолиЗа Hi1; 10 — на стадии активного гистолиЗа Hi2; 11 — на стадии продвинутого адипогенеЗа Ad1; 12 — ЗаверШенного адипогенеЗа Ad1. МасШтаб — 1 мм

opennotspecifiedOct 2014View details →

Figure 2 in Apocrine secretion of the egg hull in oogenesis and exclusion of sperm organelles at fertilization make reproduction in Chitonida (Mollusca) unique

Figure 2. (A) Portion of Figure 1C enlarged to show a region (boxed area) of apical bleb formation and aposome release from oocyte (O) into the forming hull (H). Scale bar = 2 µm. Inset: centriole (C) with rootlets in cytoplasm of late oocyte. Scale bar = 0.3 µm. (B) 3D image of microapocrine secretion from the oocyte (O) to the forming hull (H) created from an area similar to that in box in (A). Image was formed from 12 digitized serial sections entered into 'Reconstruct'. (C) TEM of Littorina sitkana bursa copulatrix, showing macroapocrine secretions (MS) being released from the cell surface (CS) to the lumen. Scale bar = 10 μm.

opennotspecifiedOct 2014View details →

Figure 1 in Apocrine secretion of the egg hull in oogenesis and exclusion of sperm organelles at fertilization make reproduction in Chitonida (Mollusca) unique

Figure 1. (A) Scanning electron micrograph (SEM) of oocyte type with closed hull cupules (C) found in some species of Acanthochitona and Cyanoplax. The example is Cyanoplax dentiens. Scale bar = 40 µm. (B) Transmission electron micrograph (TEM) of section of later stage oocyte (O) of A. garnoti showing characteristic form of closed cupules (C). FC, follicle cell. Scale bar = 10 µm. (C) TEM of forming hull cupule (H) of A. garnoti showing three areas of microapocrine secretion (arrows) from surface of oocyte (O). FN, follicle cell nucleus. Scale bar = 5 µm. (D) TEM of one region of microapocrine secretion release below the forming hull (H). Note elongate apical blebs (AB), like microvilli, extending from cell membrane of oocyte (O). Micro-aposomes are pinched off and released into the intercellular space, where the hull is forming. Note also: rare Golgi vesicle exocytosis (arrow). Scale bar = 1 µm. (E) TEM detail of

opennotspecifiedOct 2014View details →

Figure 3 in Apocrine secretion of the egg hull in oogenesis and exclusion of sperm organelles at fertilization make reproduction in Chitonida (Mollusca) unique

Figure 3. (A) SEM of egg of Stenosemus albus exhibiting the spinous hull typical of Chitonina. Scale bar = 50 µm. (B) SEM of portion of egg of S. albus showing sperm (arrow) penetrating one of a series of pores in the hull (P) located at the bases of spines. Scale bar = 5 μm. (C) Tip of needle-like extension of sperm of S. albus, showing acrosomal vesicle (AV) and tip of nuclear filament (N). Scale bar = 0.1 µm. (D) Light micrograph (LM) taken with Nomarski optics of

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Rad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish

Open the record for dataset details and reuse information.

publicJun 2021View details →

openGEO-OpenMay 2024View details →

Genome organization regulates nuclear pore complex formation and promotes differentiation during Drosophila oogenesis [CUT&RUN]

GEO Series GSE250350. Drosophila melanogaster. 24 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.

openGEO-OpenSep 2019View details →

Stage-specific combinations of opposing poly(A) modifying enzymes guide gene expression during early oogenesis

GEO Series GSE130685. Caenorhabditis elegans. 15 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenAug 2018View details →

Nuclear actin is required for transcription during Drosophila oogenesis [RNA-seq]

GEO Series GSE116363. Drosophila melanogaster. 6 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenMar 2022View details →

A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [aramis mRNA IP]

GEO Series GSE190969. Drosophila melanogaster. 9 samples. Type: Other.