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365 results for “real-time PCR”
Table S1. Geographical localization of Pinus pseudostrobus Lindley. specimens used in the study and concordance of morphological identification with real-time PCR-HRM assay for Pinus pseudostrobus varieties pseudostrobus, apulcensis, oaxacana and coatepecensis, based on the cluster pattern.
<p>File encloses geographical location of collected Pinus pseudostrobus samples in México, as well as haplotype grouping obtaines from HRM analysis</p>
Development of thrips barcode database and multiplex real-time PCR assay for quarantine and agriculture pest species
<p>Thrips (Order Thysanoptera) species are agriculturally important as plant sap sucking pests and vectors of several plant diseases. They are very small insects and commonly associated with imported commodities at New Zealand border in all life stages. Morphological identification of thrips is mainly performed on adults, but the available identification keys for immature stages do not include many species and are inadequate, thus DNA barcode was regularly used for thrips identification, here, we have generated DNA barcode data for over 29 thrips species from over 100 individuals. At New Zealand border,<em> Frankliniella occidentalis </em>is the dominant species intercepted, followed by <em>F. panamensis</em>, <em>Thrips palmi</em> and <em>T. tabaci </em>and several other thrips species. Hence, we have also developed a multiplex real time PCR assay, targeting the four thrips species to facilitate the identification of quarantine interceptions with more accurate and faster diagnostic method for any developmental stages. The DNA barcode database further assists in thrip identification. The assay showed high specificity for all the four target species and could detect 10 copies/ µL of the target DNA. Linear responses and high correlation coefficients between the amount of DNA and <em>C</em><sub>q</sub> values for each species were also achieved. The method was tested on single egg, larva and adult and proved to be applicable for all life stages of the four species. This study has demonstrated the assay is a useful biosecurity tool for rapid and reliable identification of the target thrips species. </p>
Fig. 1. A in A novel quantitative real-time PCR diagnostic assay for seal heartworm (Acanthocheilonema spirocauda) provides evidence for possible infection in the grey seal (Halichoerus grypus)
Fig. 1. A: graphical representation of cluster 20 (C20). B: representation of the selected contig and the sequence used to design the C20 quantitative real-time PCR assay. The forward primer is in bold, the reverse primer is indicated by a dotted underline, and the double-quenched probe is underlined.
Fig. 2. Standard curve generated using the log10 in A novel quantitative real-time PCR diagnostic assay for seal heartworm (Acanthocheilonema spirocauda) provides evidence for possible infection in the grey seal (Halichoerus grypus)
Fig. 2. Standard curve generated using the log10 of the ng of input A. spirocauda DNA plotted against Ct value. Unknown values are displayed as stars. For unknown samples, the total input DNA was 1 ng, which contains a mixture of seal DNA from the blood and A. spirocauda DNA. R2 = 0.985 for linear fit of standards. Curve is described by the equation y = −5.63x + 17.16, where y is the log (ng) and x is the Ct value. 95% confidence intervals are denoted by the dotted lines.
Fig. 1 in Detecting co-infections of Echinococcus multilocularis and Echinococcus canadensis in coyotes and red foxes in Alberta, Canada using real-time PCR
Fig. 1. Standard curve for qPCR assays to detect E. canadensis and E. multilocularis using Cox143 and Nad234 primers/probes, respectively.
Fig. 3 in Comparison of the modified agglutination test and real-time PCR for detection of Toxoplasma gondii exposure in feral cats from Phillip Island, Australia, and risk factors associated with infection
Fig. 3. Predicted lines of fit for the multivariable logistic regression model plotted as probability of Toxoplasma gondii qPCR positivity in feral cats on Phillip Island (Victoria) versus body weight for each season. Dashed lines show 95% confidence intervals.
Fig. 1 in Comparison of the modified agglutination test and real-time PCR for detection of Toxoplasma gondii exposure in feral cats from Phillip Island, Australia, and risk factors associated with infection
Fig. 1. Location and Toxoplasma gondii infection status, as detected by real-time PCR (qPCR), of feral cats trapped on Phillip Island (Victoria) from July 2016 to December 2017. Map shows the distribution of different location types (Park, Agricultural, Residential) used in multivariable regression analysis. A circular spread of points around a location marked with 'x' indicates multiple animals were sampled at the same site (i.e. same GPS coordinates). Red = T. gondii qPCR positive, white = T. gondii qPCR negative. Map created using Quantum GIS, version 3.8. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Fig. 2 in A novel quantitative real-time PCR diagnostic assay for fecal and nasal swab detection of an otariid lungworm, Parafilaroides decorus
Fig. 2. Standard curve based on sensitivity data. A 95% confidence interval for the linear regression model is shaded in grey.
Fig. 1. Repeat family selection for the P. decorus diagnostic assay. A in A novel quantitative real-time PCR diagnostic assay for fecal and nasal swab detection of an otariid lungworm, Parafilaroides decorus
Fig. 1. Repeat family selection for the P. decorus diagnostic assay. A. Number of sequencing reads for P. decorus compared to outgroup species reads for each repeat family (1–104) on a log scale. Arrows indicate repeat families with no reads from the outgroup species. Plot was made using Tableau Software, 2019. B. Within a cluster, reads with similar sequences are closer together. Edges connect a read with its closest match (creating a pair) and the length of this edge represents the amount of overlap between the reads. The mean edge width provides context for the lengths in the cluster, so in a cluster with a larger mean edge width the edges are actually longer than edges in a cluster with a smaller mean edge width. Reads therefore may be distant because of sequence divergence, or in the case of a long repeat (more than 150 base pairs), because of a lack of overlap between reads. However, because there will likely be continuous reads covering different regions of the repeat, these longer repeats should still appear as a tight, though possibly larger, cluster. Read dots that stray from the central cluster most likely represent sequence divergence. Higher density therefore indicates lower sequence divergence.
Fig. 4 in Giardia duodenalis in a clinically healthy population of captive zoo chimpanzees: Rapid antigen testing, diagnostic real-time PCR and faecal microbiota profiling
Fig. 4. | Faecal bacterial community profile of captive chimpanzees infected with Giardia duodenalis detected by rapid antigen test. (A) Relative abundance of colour coded bacterial phyla separated based on presence (+) or absence (‒) of Giardia using rapid antigen test (RAT). The sample identity is located at the bottom of the graph with two labels (C20, C3) shaded indicating samples that were found as Giardia positive by real-time PCR. (B) Alpha diversity based on observed OTU and Shannon's index plotted as box plot and evaluated using t-tests. (C) Principal coordinates analysis (PCoA) 2D plot using first two principal components from Bray-Curtis dissimilarity matrix at the genus taxonomic levels. The clustering between Giardia positive (RAT+) and negative (RAT-) samples was tested using ANOSIM. (D) Linear discriminant analysis effect size (LEfSe) used plot of significant factors discriminating G. duodenalis positive from negative sample. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Fig. 3 in Giardia duodenalis in a clinically healthy population of captive zoo chimpanzees: Rapid antigen testing, diagnostic real-time PCR and faecal microbiota profiling
Fig. 3. | Faecal bacterial community profile of captive chimpanzees infected with Giardia duodenalis as detected by rapid antigen test and real-time PCR combined. (A) Relative abundance of colour coded bacterial phyla separated based on presence (+) or absence (‒) of Giardia. The sample identity is located at the bottom of the graph. (B) Alpha diversity based on observed OTU and Shannon's index plotted as box plot and evaluated using t-tests. (C) Principal coordinates analysis (PCoA) 2D plot using first two principal components from Bray-Curtis dissimilarity matrix at the genus taxonomic levels. The clustering between Giardia positive (+) and negative (‒) samples was tested using ANOSIM. (D) Linear discriminant analysis effect size (LEfSe) used plot of significant factors discriminating G. duodenalis positive from negative sample. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Fig. 2 in Giardia duodenalis in a clinically healthy population of captive zoo chimpanzees: Rapid antigen testing, diagnostic real-time PCR and faecal microbiota profiling
Fig. 2. | Results of Giardia duodenalis rapid antigen test applied on faecal samples from chimpanzees. A positive result for the Giardia duodenalis rapid antigen test (RAT, Anigen Rapid Giardia AG Test Kit) is represented by the line in the 'T' position in the window along with the positive control line in the 'C' position.
Fig. 1 in Giardia duodenalis in a clinically healthy population of captive zoo chimpanzees: Rapid antigen testing, diagnostic real-time PCR and faecal microbiota profiling
Fig. 1. Captive chimpanzees and their enclosure in Sydney, Australia. (A) Main chimpanzee open air exhibit with multiple climbing structures. (B) View from the other direction showing entry to the indoor area at the end of the exhibit. (C) smaller exhibit with mesh covering and more climbing and sleeping structures. (D) Members of the chimpanzee troop at the Taronga Zoo.
Real-time RT-PCR datasets for the CEO study
<p>Real-time RT-PCR results of testing citrus essential oils for viroid transmission. </p> <p>Ct values of each run are listed in Excel sheet according to the respective experiment. </p>
Data from quantitative real-time PCR of corticotropin-releasing hormone and glucocorticoid receptor in the hippocampus and hypothalamus of rats subjected to midline fluid percussion injury or control sham surgery.
<p>These files contain all data from quantitative real-time PCR of corticotropin-releasing hormone (CRH) and glucocorticoid receptor (GR) measured in the hippocampus and hypothalamus of male and female rats. Rats were randomly assigned to a treatment group before the initiation of the study. Rats received a control sham surgery or were subjected to midline fluid percussion injury to induce a diffuse traumatic brain injury. Tissue biopsies were collected at 7 days post-injury and analyzed via quantitative real-time PCR. </p> <p> </p>
Quantitative real-time PCR assays Q2 for species-specific detection and quantification of Baltic Sea spring bloom dinoflagellates
<p>These are the data behind figures 2 to 7 in the paper: Brink AM, Kremp A and Gorokhova E (2024) Quantitative real-time PCR assays for species-specific detection and quantification of Baltic Sea spring bloom dinoflagellates. Front. Microbiol. 15:1421101. doi: 10.3389/fmicb.2024.1421101</p>
Data from: Detection of airborne genetically modified maize pollen by real-time PCR
The cultivation of genetically modified (GM) crops has raised in the European Union and other parts of the world numerous concerns about their environmental and economic impact. Especially outcrossing of genetically modified organisms (GMO) was from the beginning a critical issue as airborne pollen has been considered an important way of GMO dispersal. Here, we investigate the use of airborne pollen sampling combined with microscopic analysis and molecular PCR analysis as an approach to monitor GM maize cultivations in a specific area. Field trial experiments in the European Union and South America demonstrated the applicability of the approach under different climate conditions, in rural and semi-urban environment, even at very low levels of airborne pollen. The study documents in detail the sampling of GM pollen, sample DNA extraction and real-time PCR analysis. Our results suggest that this ''GM pollen monitoring by bioaerosol sampling and PCR screening" approach might represent an aid in managing the co-cultivation of GM and non-GM crops, especially in the surveillance of GM-free areas, centres of origin, and natural reserves.
Fig. 3 in Establishment of conventional PCR and real-time PCR assays for accurate, rapid and quantitative authentication of four mistletoe species
Fig. 3. Sensitivity and amplification efficiency of qPCR assay using species-specific primers and serial dilutions of standard DNA. PCR cycling (A, C, E, G) and melting curve (B, D, F, H) analysis of primers specific for V. coloratum using VC-F3/R4 (A, B); T. sutchuenensis var. duclouxii using TS-F3/R4-1 (C, D), K. japonica using KJ-F5-1/R4 (E, F), and L. tanakae using LT-F5/R5-1 primers (G, H). Arrows indicate the amplification curves (A, C, E, G) of serial dilutions of standard DNA with NTC. Threshold was determined according to the Rotor-Gene Q series software (version 2.3.1) manual.
Fig. 2 in Establishment of conventional PCR and real-time PCR assays for accurate, rapid and quantitative authentication of four mistletoe species
Fig. 2. Sensitivity of multiplex PCR assay in conventional PCR. M: 100 bp DNA ladder. VC: V. coloratum. TS: T. sutchuenensis var. duclouxii. KJ: K. japonica. LT: L. tanakae. (+) means samples mixed different species used in PCR template. Hyphens and arrows indicate the sizes of DNA ladder and PCR products, respectively.
Fig. 4 in Establishment of conventional PCR and real-time PCR assays for accurate, rapid and quantitative authentication of four mistletoe species
Fig. 4. Analysis of commercial mistletoe products sold as leached tea in the Korean market using multiplex PCR assay. M: 100 bp DNA ladder. C1, VC_JM; C2, VC_GY; C3, TS_HR1; C4, TS_GC2; C5, KJ_SC1; C6, KJ_PS; C7, LT_GW; and C8, LT_JC (positive control). Lanes 1–16: Commercial food and herbal medicine products distributed as authentic mistletoe. Hyphens and arrows indicate the sizes of DNA ladder and PCR products, respectively. *, inauthentic products whose identity did not match the description on the label; **, inauthentic products whose identity matched the description on the label.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.