Find research datasets worth reusing

opencc-zeroNov 2020View details →

Data from: Populus euphratica WRKY1 binds the promoter of PeHA1 to enhance gene expression and salt tolerance

Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022-bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter-reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica. PeWRKY1 binding to the PeHA1 W-box in promoter region was verified by a yeast one-hybrid assay, electrophoretic mobility shift assay, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4, which has a cis-acting W-box in the regulatory region, and H+ pumping activity in both in vivo and in vitro assays. We conclude that salt stress upregulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1. Thus, we conclude that enhanced H+ pumping activity enabled salt-stressed plants to retain Na+ homeostasis.

zenodo40/100

Raw data and scripts from de Lima et al. Succulence explains salinity tolerance in a Neotropical orchid without any evidence of local adaptation to salt spray

Open the record for dataset details and reuse information.

opencc-by-4.0Nov 2023View details →

opencc-zeroDec 2022View details →

Data for: Effects of parental age on salt stress tolerance in an aquatic plant

Parental age influences components of offspring fitness in many species. The ability to tolerate stress also affects fitness, but less is known regarding changes in offspring stress tolerance with increasing parental age, especially in plants. We examined first and fifth-born clonal offspring (using birth order as a proxy for parental age), and compared their fitness in several sub-lethal concentrations of salt (NaCl), to investigate the interactive effects of birth order and salt stress on the offspring of the aquatic plant Lemna minor L. We found that increasing salt concentration reduced reproduction particularly at early ages, which detrimentally affected fitness, as measured by the intrinsic rate of natural increase. Fifth offspring had greater fitness than first offspring, potentially due to the hump-shaped relationship between offspring fitness and birth order observed in other studies on Lemna, with fifth offspring near the peak of the hump. We found no interactive effect of birth order and salt concentration on offspring fitness; however, there were interactive effects on the time to first reproduction and the size of fronds. Specifically, first offspring exposed to increasing salt concentrations exhibited longer delays to first reproduction and grew to a greater size, while fifth offspring showed little change in either variable with increasing salt concentration. Thus, variation in birth order affected offspring response to salt stress, although not in terms of fitness. These results help illuminate factors impacting the age-specific strength of natural selection and stress responses, and may be environmentally relevant in the context of environmental salinization.

publicNov 2019View details →

Populus euphratica WRKY1 binds the promoter of PeHA1 to enhance gene expression and salt tolerance

Open the record for dataset details and reuse information.

publicDec 2022View details →

Data for: Effects of parental age on salt stress tolerance in an aquatic plant

Open the record for dataset details and reuse information.

dryad36/100

Data from: Local adaptation for enhanced salt tolerance reduces non-adaptive plasticity caused by osmotic stress

Organisms often respond to environmental change via phenotypic plasticity, where an individual modulates its phenotype according to the environment. Highly variable or changing environments can exceed physiological limits and generate maladapted plastic phenotypes, which is termed non-adaptive plasticity. In some cases, selection may reduce the negative or disruptive impacts of environmental stress and produce locally adapted populations. Salt is an increasingly prevalent contaminant of freshwater systems and can induce non-adaptive plastic phenotypes for freshwater organisms like amphibians. Hyla cinerea is a frog species with populations inhabiting brackish, coastal habitats, so we use this species to test whether coastal populations are locally adapted to tolerate saltwater by determining how salt exposure during the embryonic and larval stages alters mortality and plastic developmental and metamorphic phenotypes of coastal and inland populations. Coastal frogs have higher survival, faster growth rates, and metamorphose sooner than inland frogs across salinities. Coastal frogs also metamorphose smaller (likely a consequence of earlier metamorphosis) yet maintain constant size, while higher salinities reduce metamorphic size for inland frogs. Coastal frogs evolved to minimize non-adaptive and disruptive impacts of saltwater during larval development and accelerate the larval period to reduce time spent in a stressful environment.

opencc-zeroJun 2019View details →

ClinicalTrials.gov36/100

Multicenter Randomized Parallel Group Phase III Study Comparing the Bowel Cleansing Efficacy, Safety and Tolerability of NER1006 Versus a Sodium Picosulfate and Magnesium Salt Solution Using Day Befor

ClinicalTrials.gov study NCT02273141. IPD Sharing: Not stated. Countries: 6. Publications: 1.

restrictedIPD-UNDECIDEDFeb 2026View details →

ClinicalTrials.gov36/100

Pharmacodynamics, Safety, Tolerability, and Pharmacokinetics of Two Orally Inhaled Indacaterol Salts in Adult Subjects With Asthma.

ClinicalTrials.gov study NCT03257995. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.

restrictedIPD-UNDECIDEDFeb 2026View details →

dryad36/100

Data from: Local adaptation for enhanced salt tolerance reduces non-adaptive plasticity caused by osmotic stress

Open the record for dataset details and reuse information.

publicJun 2019View details →

dryad32/100

Dataset from: Parapatric and sympatric adaptation of Setaria viridis populations in Japan to heterogeneous coastal habitats via trait divergence of plant form, salt spray tolerance and flowering time

This study aimed to determine how coastal variants of plants arise from local populations under natural selection by studying variations in phenotypic variations and survival of Setaria viridis populations inhabiting mosaic environments of two seashores in Japan. S.viridis populations comprised five coastal variants showing significantly higher salt spray tolerance than the inland variant: ST, short and tolerant (common variant); TM, tall and mid-tolerant (Inland Sea); TT, tall and tolerant; PT, prostrate and tolerant; L, extremely late flowering; I, inland and susceptible variants. These variations imply that maritime plants first acquired salt spray tolerance for survival, after which compact plants evolved in habitats where strong winds caused damage from salt spray. Results indicate that diverse intensities of salt spray and winds as well as summer drought generated various coastal variations in parapatry and sympatry.

opencc-zeroDec 2023View details →

Fig. 8 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 8. Chlorophyll (A) and carotenoid (B) contents (mg/g FW) in pea shoots developed from not primed (NP) and primed (P) seeds at 0 and 120 mM NaCl.The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 10 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 10. Total flavonoid content (mg QE/g FW) in roots and shoots of seedlings developed from not primed (NP) and primed (P) pea seeds at 0 and 120 mM NaCl. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 11 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 11. Total precipitable alkaloids content (mg PAHE/g FW) in roots and shoots of seedlings developed from not primed (NP) and primed (P) pea seeds at 0 and 120 mM NaCl. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 7 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 7. Sodium (a), potassium (b), and phosphor (c) content (in %) in (A) pea germinated not primed (NP) and primed (P) seeds and (B) in shoots developed from P and NP seeds, in the presence of NaCl at different concentrations. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 5 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 5. Proline content (μmol/g DW) in (A) pea germinated not primed (NP) and primed (P) seeds and (B) in roots and shoots of seedlings developed from P and NP seeds,in the presence of NaCl at different concentrations. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 6 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 6. Total soluble sugars content (mg/g FW) in (A) pea germinated not primed (NP) and primed (P) seeds and (B) in roots and shoots of seedlings developed from P and NP seeds, in the presence of NaCl at different concentrations. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 3 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 3. Content of malondialdehyde (MDA) (U/g FW) in (A) pea germinated not primed (NP) and primed (P) seeds and (B) in roots and shoots of seedlings developed from P and NP seeds, in the presence of NaCl at different concentrations. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 2 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 2. Electrolyte leakage (%) of (A) pea germinated not primed (NP) and primed (P) seeds and (B) and pea roots and shoots of seedlings developed from P and NP seeds in the presence of NaCl at different concentrations, after an immersion of 24 and 48 h in distilled water. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.

Fig. 1 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 1. Roots and shoots length, developed from primed seeds, at 0 and 120 mM NaCl, expressed in percent of control (seedlings developed from not primed (NP) seeds). Value (N = 4 ± S.E.). Different letters in columns indicate significant differences at p <0.05.

Fig. 9 in Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract

Fig. 9. Total phenolic content (mg GA/g FW) in roots and shoots of seedlings developed from not primed (NP) and primed (P) pea seeds at 0 and 120 mM NaCl. The bars on each column show standard error. Value = average ± S.E., n = 4. Different letters on columns indicate significant differences among treatments at p <0.05.