Find research datasets worth reusing

Fig. 6 in A previously undescribed phenylethanoid glycoside from Callicarpa kwangtungensis Chun acts as an agonist of the Na/K-ATPase signal transduction pathway

Fig. 6. Effect of callicarpanoside B on ERK activation in AAC-19 cells. AAC-19 cells were treated with or without 100, 1000 nM callicarpanoside B for 10 min, and the cell lysate was collected for Weston blot analysis showed in the upper panel. The statistics were combined from 3 separate experiments and are presented as mean ± SEM, showed in the lower panel. Student t-test, *, P <0.05; **, P <0.01.

Fig. 7 in A previously undescribed phenylethanoid glycoside from Callicarpa kwangtungensis Chun acts as an agonist of the Na/K-ATPase signal transduction pathway

Fig. 7. The relative binding affinity of callicarpanoside B compared with ouabain. The 3H-ouabain binding assay was utilized to determine the relative binding affinity. After preincubation with non-radioactive ouabain or callicarpanoside B to NKA for 30 min in cultured LLC-PK1 cells, 100 nM 3H-ouabain were added and incubated another 30 min. Thus, the IC50 for binding nonradioactive ouabain or callicarpanoside B to NKA was determined by competition with 3H-ouabain. The data are combined from three experiments and are presented as mean ± SD. Non-radioactive ouabain, IC50 = 0.95 ± 0.35 μM; callicarpanoside B, IC50 = 10.55 ± 3.05 μM.

Fig. 5 in A previously undescribed phenylethanoid glycoside from Callicarpa kwangtungensis Chun acts as an agonist of the Na/K-ATPase signal transduction pathway

Fig. 5. Effect of callicarpanoside B on NKA-mediated kinase activation in LLC-PK1 cells. A, LLCPK1 cells were treated with 10, 100, and 250 nM callicarpanoside B for 10 min, and the cell lysate was collected for Weston blot analysis showed in the upper panel. The data are combined from 3 to 8 separate experiments and are presented as mean ± SEM, shown in the lower panel. B, LLCPK1 cells were treated with 100 nM callicarpanoside B for 2, 10, 30, and 120 min, and the cell lysate was collected for Weston blot analysis, showed in the upper panel. The data are combined from 3 to 8 separate experiments and are presented as mean ± SEM. C, LLCPK1 cells were treated with 10, 100, and 250 nM callicarpanoside B for 10 min, and the cell lysate was collected for Weston blot. Probed for p-Src and showed in the upper panel. The data are combined from indicated separate experiments and are presented as mean ± SEM, shown in the lower panel. D, LLCPK1 cells were treated with 10, 100 nM callicarpanoside B for 15 min, and the lysates were fractioned into Cytosolic (Cy) and particulate (Pa) fractions for Weston blot analysis and Pa/Cy ratios of PKCε, contents were compared. The upper panel represents Western blot. The data are combined and presented as mean ± SEM of 3 independent experiments, shown in the lower panel. Student t-test, *, P <0.05; **, P <0.01.

Fig. 4 in A previously undescribed phenylethanoid glycoside from Callicarpa kwangtungensis Chun acts as an agonist of the Na/K-ATPase signal transduction pathway

Fig. 4. Effect of callicarpanoside B on NKA endocytosis. A, TCN-YFP-α1 cells were treated with callicarpanoside B for 6 h. Cells were fixed with Methanol. Coverslips were mounted and imaged under a fluorescence microscope described previously (Liang et al., 2006; Tian et al., 2006). B, cell surface NKA α1 was measured by biotinylation assay as described under "Experimental". Proteins were collected after treatment with different concentrations of callicarpanoside B, ouabain as a control. Cell lysates were separated by SDS-PAGE and analyzed by Western blot for NKA α1. D, quantitative dose-response endocytosed NKA α1 was calculated from four to eight independent experiments for each dose. The values are mean ± SEM. Two-way ANOVA, **, P <0.01; ***, P <0.001.

Fig. 3 in A previously undescribed phenylethanoid glycoside from Callicarpa kwangtungensis Chun acts as an agonist of the Na/K-ATPase signal transduction pathway

Fig. 3. Concentration curve of callicarpanoside B (2) on NKA inhibition. The purified NKA was incubated with different concentrations of compounds for 15 min, then assayed for ouabain-sensitive ATPase activity as described under Materials and Methods. The data are combined from three to five separate experiments and are presented as mean ± SEM.

Fig. 8 in A previously undescribed phenylethanoid glycoside from Callicarpa kwangtungensis Chun acts as an agonist of the Na/K-ATPase signal transduction pathway

Fig. 8. Effect of callicarpanoside B on cell growth. LLCPK1 cells were subcultured in 12-well plates (50,000 cells/well) and serum-starved overnight. Before treatment, three wells of day 0 were trypsinized and counted. After callicarpanoside B treatment at the indicated dose, three wells of individual control (Con) and compound-treated cells were trypsinized and counted at 48 h.

zenodo28/100

Fig. 2. Key 1H–1H in A previously undescribed phenylethanoid glycoside from Callicarpa kwangtungensis Chun acts as an agonist of the Na/K-ATPase signal transduction pathway

Fig. 2. Key 1H–1H COSY and HMBC correlations of compounds 1 and 2.

zenodo28/100

Fig. 1 in A previously undescribed phenylethanoid glycoside from Callicarpa kwangtungensis Chun acts as an agonist of the Na/K-ATPase signal transduction pathway

Fig. 1. Chemical Structure of compounds 1–14.

publicMay 2009View details →

dryad28/100

Data from: Contrasting selection pressures on components of the Ras-mediated signal transduction pathway in Drosophila

Open the record for dataset details and reuse information.

openGEO-OpenAug 2015View details →

Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses [Expt: siRNA]

GEO Series GSE39963. Homo sapiens. 6 samples. Type: Expression profiling by array.

openGEO-OpenApr 2018View details →

Enhanced wound healing of tissue-engineered human corneas through altered phosphorylation of the CREB and AKT signal transduction pathways

GEO Series GSE113438. Homo sapiens. 8 samples. Type: Expression profiling by array.

Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses [Expt: ChIP-chip]

GEO Series GSE39960. Homo sapiens. 6 samples. Type: Genome binding/occupancy profiling by genome tiling array.

openGEO-OpenAug 2015View details →

Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses [Expt: PLZF_KELLY]

GEO Series GSE39964. Homo sapiens. 4 samples. Type: Expression profiling by array.

Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses [Expt: Geni_Bafi]

GEO Series GSE39962. Homo sapiens. 8 samples. Type: Expression profiling by array.

openGEO-OpenMay 2022View details →

Supplementation of specific collagen peptides following high-load resistance exercise upregulates gene expression in pathways involved in skeletal muscle signal transduction

GEO Series GSE195585. Homo sapiens. 119 samples. Type: Expression profiling by high throughput sequencing.

Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses

GEO Series GSE39965. Homo sapiens. 30 samples. Type: Genome binding/occupancy profiling by genome tiling array; Expression profiling by array.

openGEO-OpenFeb 2013View details →

Studies of target genes for signal transduction pathways (using a Ras1-, a Cdc42- and a Gap1-mutant) in the basidiomycete Schizophyllum commune.

GEO Series GSE43965. Schizophyllum commune. 6 samples. Type: Expression profiling by array.

openGEO-OpenJul 2020View details →

Genome-wide identification and characterization of Gibberellin Metabolic and Signal Transduction (GA MST) pathway mediating seed and berry development (SBD) in Grape (Vitis vinifera L.)

GEO Series GSE153169. Vitis vinifera. 1 samples. Type: Expression profiling by high throughput sequencing.

Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses [Expt: PLZF_HEK]

GEO Series GSE39961. Homo sapiens. 6 samples. Type: Expression profiling by array.