FIGURE 7 in The description of a cryptic species related to the pan-Amazonian frog Allobates femoralis (Boulenger 1883) (Anura: Aromobatidae)

FIGURE 7. Maximum Likelihood phylogenetic tree reconstructed from unique haplotypes of a 518 b.p. fragment of the mitochondrial gene 16S rRNA of Allobates femoralis and Allobates hodli sp. nov. sampled in 13 localities in Brazilian Amazon. Data set included reference sequences from Peru, Colombia, Ecuador, Suriname and other localities in Brazil obtained from GenBank. (A) Phylogenetic position of the clade including A. hodli sp. nov. and A. femoralis in relation to outgroups supports Allobates zaparo as their sister group. Clade labels represent support values from 5000 bootstrap replicates (only values above 50 are shown). (B) Relative phylogenetic placement of clades within the ingroup. Sample oscillograms and sonograms of advertisement calls from populations within clades display natural variation in number of notes. A. hodli is closely related to populations of A. femoralis from the southern Brazilian state of Acre (clade Acre 01), which present a four-note advertisement call and color pattern characteristic of A. femoralis. Both form the sister group to samples from northwestern Acre and from the Madre de Dios River basin (clade Acre 02). The basal clade containing A. hodli and Acre 01 and Acre 02 clades is the sister group to a basal clade containing A. femoralis sensu stricto (placed in the weakly supported clade femo 04) and the other reference sequences from populations referred to as A. femoralis (clades femo 01, femo 02, femo 03, femo 04). Samples from Ecuador form a divergent and well supported clade, with advertisement calls formed by a single note. Individuals from Panguana (placed in clade femo 04) present a distinctive 3- note advertisement call (not shown). All remaining populations have advertisement calls constituted by four notes. Calls from Yasuní, Ecuador, published by Read (2000). Calls from Pongo de Cainarachi, Peru (about 45 km south from A. femoralis type-locality in Yurimaguas) provided by Jason L. Brown. (C) Corresponding values on axes of oscillograms and sonograms of advertisement call samples.