Figure 5 in Tetraploidy in the Boettger's dwarf clawed frog (Pipidae: Hymenochirus boettgeri) from the Congo indicates non-conspecificity with the captive population

Figure 5. Hymenochirus sp. (IVB-H-Hsp06, female), sequential fluorescent chromosome mapping (DAPI, CMA3, C-banding, rDNA FISH), non-sequential snDNA FISH, and whole-genome painting on metaphase spread. A, DAPI (B&W) counter-stained metaphase spread shows all 21 chromosomes. B, CMA3 banding in green shows NOR locus on the p arm of chromosome 4. CMA3 signal co-localizes with 28S locus. C, C-banding (B&W, brighter staining) highlights heterochromatic blocks on telomeric and pericentromeric regions of almost all chromosomes. In addition, the whole B chromosome is intensely banded (arrow). D, 5S (green) and 28S (red) rDNA loci are located on the q arm of chromosome 6 and p arm of chromosome 4, respectively. The 5S rDNA is situated on two different chromosomal loci within the single q arm. E, the snDNA loci U1 (red) and U2 (green) are located on the p arm of chromosome 1 and the q arm of chromosome 8, respectively. F, the GISH experiment of the H. boettgeri whole-genome painting probe, which hybridizes on Hymenochirus sp. chromosomes. All chromosomes are painted (red) except one, B chromosome, which shows no GISH signal and is DAPI-positive (arrow). Scale bars represent 10 μm.