Figure 4 in Agrobacterium-mediated gene delivery and transient expression in the red macroalga Chondrus crispus

Figure 4: Effect of cultivation medium, wounding method and acetosyringone on Agrobacterium transformation efficiency. The transformation efficiencies of Chondrus thalli segments co-cultivated with pCAMBIA 1301-transformed Agrobacterium in seawater and Induction Medium (IM) were compared. Using seawater as co-cultivation medium, the wound-related treatments, namely biolistics wounding, wounding using pin-pricks and no wounding, were compared. The effect of acetosyringone (100 µM) was also studied. Data are expressed as means ± SE (n = 4). The Mann-Whitney non-parametric test was conducted to compare between two groups.