Imaging dataset 01 for mtFociCounter
<p>This is the first dataset associated with the updated manuscript on mtFociCounter.</p> <p>Images are Spinning Disk Confocal Images of unsorted 3t3 NIH mouse fibroblasts stably expressing <strong>mitochondrially</strong> targeted dsRed, immunofluorescence against dsDNA (mitochondrial <strong>nucleoids</strong>) and AlexaFluor 647, and stained <strong>nuclei</strong> with Hoechst.</p> <p>It contains all data necessary to reproduce the analysis of 3t3 WT sampling (sample-to-sample comparison), as described in the manuscript. "samples" from the same day are 13mm coverslips processed in parallel (seeding of cells, fixation, immunofluorescence) and mounted on the same glass slide (#1 on left, #2 centre, #3 right). The dataset contains the 3t3 WT data from the following acquisition dates:</p> <p>20220603_sample2<br> 20220603_sample3<br> 20220706_sample2<br> 20220706_sample3<br> 20220708_sample1<br> 20220708_sample2<br> 20220708_sample3<br> 20221022_sample2<br> 20221022_sample3</p> <p>For details of the sampling procedure, please refer to the accompanying manuscript, which will soon be made available on BioRxiv.<br> </p>
ShareScore
36/100
Overall dataset sharing score
Score breakdown
These five areas show where the dataset supports — or may limit — practical reuse.
- Stewardship
- 8
- Harmonization
- 4
- Access
- 16
- Reuse readiness
- 8
- Engagement
- 0