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Figure 3 in Differential gene expression pattern and plasma sex steroids during testicular development in Genyatremus luteus (Perciforme: Haemulidae) (Bloch, 1790)
Figure 3. Gene expression of er (A) andlhr (B) in the male gonad of Genyatremus luteus individuals at different maturation stages. Columns represent arithmetic mean and standard error of the mean. abc Different letters indicate statistically significant difference (p<0.05).
Figure 2 in Saccharomyces cerevisiae OS303 expression of an alkaline protease from a newly isolated Bacillus subtilis D9
Figure 2. Analysis cloning vector construction containing alkaline protease gene with restriction enzymes to confirm the integrity of the PCR product of alkaline protease gene and to design the appropriate primers to construct the expression vector. By the enzyme combination of NheI- NdeI- BamHI restriction enzymes showed two bands of approximately 3000bp and ~1300bp must be shown as alkaline protease gene. Lane 1: DNA size marker hyperladder I; Lane 2, 3, 4, and 5: Digested sample of TA Cloning vector construction containing alkaline protease with enzyme SpeI, EcoRI, XbaI, and NheI- NdeI- BamHI respectively.
Figure 3 in Saccharomyces cerevisiae OS303 expression of an alkaline protease from a newly isolated Bacillus subtilis D9
Figure 3. Analysis of expression cloning vector (digested and purified cloning vector pRS426/GAL1p-207-Glu-MS ligated with alkaline protease called in this study pRS426/GAL1p-207-Glu-MS/ alkaline-protease plasmids. To screen the recombinant plasmids, the plasmids prepared from colonies were digested with double restriction enzymes and then were run on 0.8% agarose gel electrophoresis. Lane 1: DNA size marker hyperladder I; Lane 2, 3: pRS426/GAL1p-207-Glu-MS/alkaline-protease plasmid digested with BamHI and SpeI, after digestion, two bands of>6000bp and <1500bp observed.
Figure 10 in Evaluation of the Chilli veinal mottle virus CP gene expressing transgenic Nicotiana benthamiana plants for disease resistance against the virus
Figure 10. Symptoms development in transgenic plants after challenging with ChiVMV isolate ATIPK at15dpi. A) Resistance transgenic lines. B) Moderately resistance transgenic lines. C) Moderately susceptible transgenic lines.
Figure 8 in Evaluation of the Chilli veinal mottle virus CP gene expressing transgenic Nicotiana benthamiana plants for disease resistance against the virus
Figure 8. PCR products from transgenic plants. (a) Lane 1-3, represent PCR product from agro-infilterated plants. Lane 4, control plant. (b) Lane 1-9, RT- PCR product from transgenic plants represent the presence of CP gene transcription. Lane 10, represents untransformed control sample.
Figure 5 in Evaluation of the Chilli veinal mottle virus CP gene expressing transgenic Nicotiana benthamiana plants for disease resistance against the virus
Figure 5. Maximum likelihood tree of ChiVMV isolate ATIPK on nucleotide sequence of CP gene with 22 ChiVMV isolates from the world. The bootstrap analysis was conducted in 1000 replications. ZYMV (AB127936) sequence is used as out-group.
Figure 4 in Evaluation of the Chilli veinal mottle virus CP gene expressing transgenic Nicotiana benthamiana plants for disease resistance against the virus
Figure 4. RT-PCR amplification of the CP gene with CVMV1037/ oligo(dT). Lane 1, 2, 3 and 4 contains 1.2kb amplified product of NIb and CP gene.
Figure 4 in TiO nanoparticles and salinity stress in relation to artemisinin production and ADS and DBR2 expression in Artemisia absinthium L.
Figure 4. The effect of salinity and titanium dioxide nanoparticles on DBR2 gene expression in wormwood (the non–identical letters indicate significant difference based on Duncan test P≤ 0.05).
Data from: miRglmm: a generalized linear mixed model of isomiR-level counts improves estimation of miRNA-level differential expression and uncovers variable differential expression between isomiRs
<p>These datasets can be used to reproduce all analyses from the publication "miRglmm: a generalized linear mixed model of isomiR-level counts improves estimation of miRNA-level differential expression and uncovers variable differential expression between isomiRs" in conjunction with codes found at https://github.com/mccall-group/miRglmm_paper. </p> <p>"Monocyte_data_subset.rda", "monocyte_exact_subset_filtered2.rda" and "sims_N100_m2_s1_rtruncnorm.rda" can be used to reproduce the simulation analysis. </p> <p>"panel_B_SE.rda" and "ERCC_filtered.rda" can be used to reproduce the ERCC synthetic data analysis with known ground truth.</p> <p>"study89_data_subset.rda" and "study89_data_subset_filtered2.rda" can be used to reproduce the immune cell-type analysis. </p> <p>"bladder_testes_data_subset.rda" and "bladder_testes_data_subset_filtered2.rda" can be used to reproduce the bladder vs testes tissue analysis.</p>
Fig. 4 in Successful transcription but not translation or assembly of Solenopsis invicta virus 3 in a baculovirus-driven expression system
Fig. 4. Quantitative PCR (absolute) results evaluating transcript production of SINV-3 by AcSINV-3-infected Sf21 cells. RNA preparations from AcSINV-3 (2 rep- licates, Ci and Di) were treated with DNase I, reverse transcribed, and amplified by qPCR. Results were compared with a series of plasmid constructs containing a portion of the SINV-3 genome (102–109 genome equivalents). The region amplified was at the 3'-most end of ORF2 (see Fig. 2). No amplification was detected in polyhedrin-negative AcRP23.lacZ preparations.
Fig. 6 in Expression profile of two HSP70 chaperone proteins in response to extreme thermal acclimation in Xestia c-nigrum (Lepidoptera: Noctuidae)
Fig. 6. Xc-HSP70 mRNA expression profiles induced by cold (−7 to 5 °C) and heat (37 to 47 °C) in 2nd, 3rd, 4th, 5th, and 6th instars and pupae of Xestia cnigrum. The relative quantities indicate the levels of the HSP70 gene transcript normalized against transcript levels of β-actin as an internal standard and compared with the transcript levels of the untreated control at 25 °C. An asterisk indicates a significant difference between the control and heat shock conditions (significant, * P <0.05). The data are denoted as the mean ± SEM (error bar).
Fig. 5 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)
Fig. 5. Relative expressional levels of HSP90 mRNA in P. citri adult females afer treatment at 32 °C with abamectin concentrations of LC25, LC50 and LC75 for the indicated times. Data are shown as means of 3 replicates ± SD. The relative expression level of HSP90 mRNA in the control group is equal to 1.0 and is marked with a dashed line. The asterisk above the bar indicates a significance difference between treatment with abamectin and control group (t-test, P <0.05).
Fig. 3 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)
Fig. 3. Relative expressional levels of HSP90 mRNA in P. citri adult females afer treatment with abamectin at LC25, LC50 and LC75levels for the indicated times at 18 °C.Data are shown as means of 3 replicates ± SD. The relative expression level of HSP90 mRNA in the control group is equal to 1.0 and is marked with a dashed line. The asterisk above the bar indicates a significance difference between treatment with abamectin and control group (t-test, P <0.05).
Fig. 1 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)
Fig. 1. The cDNA and deduced amino acid sequence of HSP90 of Panonychus citri adult females. The start and stop codons are boxed; the numbers on the lef are for the positions of nucleotides and amino acids in the sequences.
Fig. 7 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)
Fig. 7. Relative expressional levels of HSP90 mRNA in adult female P. citri Mc- Gregor afer treatment with dsRNA at concentrations equivalent to 0.1 µg/µL, 0.2 µg/µL and 0.4 µg/µL. Data are shown as means of 3 replicates ± SD. The relative expression level of HSP90 mRNA in the control group is equal to 1.0 and is marked with a dashed line. The asterisk above the bar indicates a significance difference between treatment with dsRNA and control group (t-test, P <0.05).
Fig. 4 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)
Fig. 4. Relative expressional levels of HSP90 mRNA in P. citri adult females afer treatment at 25 °C with abamectin concentrations of LC25, LC50 and LC75 for the indicated times. Data are shown as means of 3 replicates ± SD. The relative expression level of HSP90 mRNA in the control group is equal to 1.0 and is marked with a dashed line. The asterisk above the bar indicates a significance difference between treatment with abamectin and control group (t-test, P <0.05).
Fig. 6 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)
Fig. 6. Relative expressional levels of HSP90 mRNA in P. citri adult females subjected to 18 °C or 32 °C for the length of time indicated. Data are shown as means of 3 replicates ± SD. The relative expression level of HSP90 mRNA in the control group is equal to 1.0 and is marked with a dashed line. The asterisk above the bar indicates a significance difference between treatment with temperature and control group (t-test, P <0.05).
Table S5 Differentially Expressed genes
Open the record for dataset details and reuse information.
Fig. 9 in A mosaic of conserved and novel modes of gene expression and morphogenesis in mesoderm and muscle formation of a larval bivalve
Fig. 9 Comparative expression of Mox in Metazoa. X: absence of a Mox ortholog in the genome,?: no data available. Phylogeny after Laumer et al. (2019). Comparative analysis suggests that Mox has an
Fig. 1 in A mosaic of conserved and novel modes of gene expression and morphogenesis in mesoderm and muscle formation of a larval bivalve
Fig. 1 Expression of Brachyury (Dro-Bra) in Dreissena rostriformis gastrula and trochophore stages. Anterior is up. Black arrowheads indicate the blastopore/stomodaeum, sf marks the shell field, dotted line marks the region of the prototroch (pt). Scale bar equals 20 µm. a Lateral view of Dro-Bra in the ventral mesoderm. b Ventral view of mesodermal Dro-Bra expression. c Dro-Bra is expressed in the ventro-posterior mesoderm and in the developing foregut. d Mesodermal and endodermal expression of Dro-Bra, ventral view. The left and right lobes belong to the prototroch (pt). A, anterior; D, dorsal; P, posterior; V, ventral
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.