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opencc-by-4.0Aug 2021View details →

Source data for Incarbone et al (2021) - "Immunocapture of dsRNA-bound proteins provides insight into tobacco rattle virus replication complexes and reveals Arabidopsis DRB2 to be a wide-spectrum antiviral effector"

<p>Source data for Incarbone et al (2021) - "Immunocapture of dsRNA-bound proteins provides insight into tobacco rattle virus replication complexes and reveals Arabidopsis DRB2 to be a wide-spectrum antiviral effector"</p> <p>Includes full scans of blots mounted in figures and additional microscopy acquisitions, including brightfield channel</p>

Fig. 4 in An electrophysiological characterization of naturally occurring tobacco alkaloids and their action on human α4β2 and α7 nicotinic acetylcholine receptors

Fig. 4. Concentration–response curves were obtained by using the whole-cell mode of the patch-clamp technique at a holding potential of −70 mV from CHO cells stably expressing human α4β2 (continuous black lines) or human α7 (dotted grey lines) nAChRs. The lines represent the fits to the Hill equation for nicotine (1) (circles symbols), anabasine (12) (triangles symbols), nornicotine (7) (squared symbols), and S-anatabine (16) (diamonds symbols). The fit parameters are presented (Table 1). The averaged normalized current response is plotted as a function of the maximal current to ACh (ImaxACh). Two backgrounds highlight the concentration range of the tobacco alkaloids levels measured in human plasma (grey background with diagonal lines) and in human cerebrospinal fluid (grey background with horizontal lines). Data are presented as mean ± SD.

Fig. 3 in An electrophysiological characterization of naturally occurring tobacco alkaloids and their action on human α4β2 and α7 nicotinic acetylcholine receptors

Fig. 3. Sequential applications of brief ACh pulses are plotted as a function of time for three ACh concentrations. (a) EC20 = 0.4 μM (circles), EC50 = 1.2 μM (squares), EC90 = 11.1 μM (triangles) for human α4β2 receptors. (b) EC20 = 33.3 μM (circles), EC60 = 300 μM (squares), EC90 = 900 μM (triangles) for human α7 receptors. Experiments were performed by using the whole-cell mode of the patch-clamp technique at a holding potential of −70 mV in CHO cells stably expressing the human α4β2 and α7 nAChRs. EC, effective concentration. Data are presented as mean ± SD.

Fig. 2 in An electrophysiological characterization of naturally occurring tobacco alkaloids and their action on human α4β2 and α7 nicotinic acetylcholine receptors

Fig. 2. Representative traces of currents elicited by using the agonist mode (a and c) and PAM mode (b and d) in the whole-cell mode of the patch-clamp technique at a holding potential of −70 mV in CHO cells stably expressing human α4β2 (a and b) and human α7 (c and d) nAChRs. Agonist mode (a, c): 0.3% DMSO (left); 33.3 μM of the tobacco alkaloid anabasine (middle); 33.3 and 900 μM ACh for α4β2 and α7, respectively (right). PAM mode (b, d): 0.4 and 100 μM ACh for α4β2 and α7, respectively (left); co-application of 33.3 μM of the tobacco alkaloid trans-anatalline (middle); 0.4 or 100 μM ACh for α4β2 and α7, respectively (right). Thick dotted lines represent the pre-application period of the alkaloid (≥2 min). Thin dotted lines represent zero current.; PAM, positive allosteric modulator.

Fig. 1 in An electrophysiological characterization of naturally occurring tobacco alkaloids and their action on human α4β2 and α7 nicotinic acetylcholine receptors

Fig. 1. Representative traces of currents elicited in response to increasing concentrations of ACh. Currents were measured in agonist mode by using the whole-cell mode of the patch-clamp technique at a holding potential of −70 mV in CHO cells stably expressing human α4β2 (a) and human α7 (b) nAChRs. The half-maximal effective concentrations determined by using the Hill equation were 1.02 ± 1.0 μM (a) and 142 ± 16 μM (b) (n = 5–11 cells). Bars indicate (in μM): 0.1 (1), 0.4 (2), 1.2 (3), 3.7 (4), 11.1 (5), 33.3 (6), 100 (7), 300 (8), 900 (9), and 2700 (10). Dotted lines represent zero current.

opennotspecifiedJan 2023View details →

Fig. 4 in The anti-TMV potency of the tobacco-derived fungus Aspergillus versicolor and its active alkaloids, as anti-TMV activity inhibitors

Fig. 4. Docking poses (left) and interactions (right) of compound 10 (yellow) at the binding site of TMV (blue, PDB code 2OM3). The ligand with TMV A chain amino GLY-135 (1.92 Å) form three hydrogen bonds (green dotted line), Hydrogen bonding distance had been hidden for clarity. The binding energy of compound 10 for between ligands and TMV. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

opennotspecifiedJan 2023View details →

Fig. 2. The key 1H–1H in The anti-TMV potency of the tobacco-derived fungus Aspergillus versicolor and its active alkaloids, as anti-TMV activity inhibitors

Fig. 2. The key 1H–1H COSY (bold lines), HMBC (solid arrows), and ROESY (dashed arrows) correlations of compounds 1–5.

opennotspecifiedJan 2023View details →

Fig. 3 in The anti-TMV potency of the tobacco-derived fungus Aspergillus versicolor and its active alkaloids, as anti-TMV activity inhibitors

Fig. 3. Docking poses (left) and interactions (right) of compound 1 (yellow) at the binding site of TMV (blue, PDB code 2OM3). The ligand with TMV. A chain amino ASN-73 (2.81 Å) and TYR-139 (3.04 Å) form four hydrogen bonds (green dotted line), Hydrogen bonding distance had been hidden for clarity. The binding energy of strong affinity between ligands and TMV. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article

Fig. 2 in Zinc uptake and HMA4 activity are required for micro- and macroelement balance in tobacco (Nicotiana tabacum)

Fig. 2. Classification of gene expression changes observed in both the HMA4 double-knockout mutants (cv. #3) and HMA4 RNAi plants (cv.#1) relative to their respective controls. The genes were classified manually according to the putative function of the closest Arabidopsis Blast hit (see Supplementary Table S2).

Fig. 1 in Zinc uptake and HMA4 activity are required for micro- and macroelement balance in tobacco (Nicotiana tabacum)

Fig. 1. (a) Relative element contents of HMA4-impaired plants compared with their controls. HMA4 RNAi plants in cv.#1 background were compared with their WT controls (n = 3 replicates each), and HMA4 double-mutant plants in cv. #3 background were compared with their null-segregant controls (n = 4). The elements were measured in dry weight of lower leaves of plants grown under greenhouse conditions. The error bars depict the 0.95 confidence interval (CI) corrected according to Satterthwaite approximation. (b) Pictures of representative plants of each group (sticker size = 11.9 cm).

Fig. 6 in Zinc uptake and HMA4 activity are required for micro- and macroelement balance in tobacco (Nicotiana tabacum)

Fig. 6. Relative element contents of different HMA4-double mutants versus the null-segregant WT controls in field experiments. The data were consolidated over 4 years of field experiments in Switzerland and 3 years of field experiments in Poland, as described in the Experimental section. The elements were measured in dry weight of leaves (mid-lower leaf position). Bars indicate estimates and confidence intervals (α = 0.1).

Fig. 4 in Zinc uptake and HMA4 activity are required for micro- and macroelement balance in tobacco (Nicotiana tabacum)

Fig. 4. Relative elemental content of N. tabacum cv. #1 and cv. #2 HMA4 RNAi plants compared with their WT and of HMA4 double-knockout mutants compared with their null-segregants, for four different fertilization conditions: (a) normal fertilization, (b) fertilization containing lower concentrations of phosphate, (c) fertilization containing higher concentrations of Zn, and (d) fertilization with low concentrations of phosphate and high concentrations of Zn. The high Zn condition was achieved by addition of 0.1 g additional Zn per plant. The elements were measured in dry weight of leaves (mid-lower leaf position). Absolute data are listed in Supplementary Table S3. Data represent mean (0.95 CI) of n = 5 replicate plants, corrected according to Satterthwaite approximation. (Three outliers were removed for condition (d): Al and Cu for cv.#2; Ti for cv.#3.).

Fig. 3 in Zinc uptake and HMA4 activity are required for micro- and macroelement balance in tobacco (Nicotiana tabacum)

Fig. 3. Working model of metabolic changes in HMA4-impaired plants based on the gene expression changes observed in the roots and leaves of HMA4 RNAi and HMA4-mutant N. tabacum plants relative to their controls. The lack of HMA4 function causes Zn deficiency in leaves, leading to a local Zn-deficiency response. In roots, a Fe-deficiency response is observed, caused either by a high Zn:Fe ratio in the roots or by a systemic Zn-deficiency signal. The Fe-deficiency signal leads—via the transcription factor FIT1—to upregulation of the Fe- and Mn- transporter genes IRT1 and NRAMP1 and also induces the synthesis and export of iron-binding compounds (IBC), thus additionally assisting Fe uptake. At the same time, a P-deficiency signal involving the transcription factor HHO2 is generated in the leaves. In roots, the P-deficiency signal leads to increased expression of genes encoding acid phosphatases, enabling increased phosphate uptake from the rhizosphere. Consequently, Fe, Mn, Cu, and P accumulate in the leaves. P is stored in vacuoles (via PHT5; 1, PHT5; 3) and also exported to mitochondria and chloroplasts. Cu is detoxified by transport to the chloroplasts, which are a major site of Cu use because of Cu-containing plastocyanin production. However, high levels of Cu are toxic for the photosynthetic electron transport system, especially for photosystem II, which is reflected in the upregulation of PII light harvesting complex genes (LHCB). At the same time, Cu uptake to the cells is limited by downregulation of COPT1. Ion uptake might cause a lower osmotic potential, leading to water influx via various aquaporin genes. High turgor pressure or Fe excess ultimately affects the cell wall, as observed by the increased expression of genes involved in cell wall biosynthesis, expansion, and crosslinking. bHLH115: basic helix-loop-helix transcription factor; CA: carbonic anhydrase; CESA: cellulose synthase; COPT: copper transporter; DMRL: dimethyl-8-ribityllumazine synthase; EXPA: expansin; FIT: FER-like regulator of iron uptake (transcription factor); HHO2: hypersensitivity to low phosphate-elicited primary root shortening 1 homolog 2 (myb-like transcription factor); HMA: heavy metal ATPase; IBC: iron binding compound; IRT: iron-regulated transporter; LAC: laccase; LHCB: photosystem II light harvesting complex; NA: nicotianamine; NAS: nicotianamine synthase; NRAMP: natural resistance-associated macrophage protein (metal ion transporter); PDR: pleiotropic drug resistance (ATP-binding cassette transporter); PHT: phosphate transporter; PIP: plasma membrane intrinsic protein; PME: pectin methyl esterase; PMEI: pectin methyl esterase inhibitor; PRX: peroxidase; RBCS: ribulose bisphosphate carboxylase small chain; TIP: tonoplast intrinsic protein; TBL: TRICHOME BIREFRINGENCE-LIKE (xylan acetylation); ZIP: zinc transporter.

Combining Default Choices and a Decision Aid to Improve Tobacco Cessation

ClinicalTrials.gov study NCT04868474. IPD Sharing: NO. Countries: 2. Publications: 3.

restrictedIPD-UNDECIDEDFeb 2026View details →

Diet Intervention Spirometry and Tobacco

ClinicalTrials.gov study NCT02151669. IPD Sharing: Not stated. Countries: 1. Publications: 3.

Identify the Usefulness of Peer-facilitated Versus Self-navigated Quit Tobacco Program for Youths.

ClinicalTrials.gov study NCT06053762. IPD Sharing: NO. Countries: 1. Publications: 22.

restrictedIPD-UNDECIDEDFeb 2026View details →

Using Non-invasive Brain Stimulation (tDCS) With Varenicline for Treating Tobacco Dependence

ClinicalTrials.gov study NCT03841292. IPD Sharing: Not stated. Countries: 1. Publications: 1.

Adapting and Evaluating a Brief Advice Tobacco Intervention in High-Reach, Low-Resource Settings in India

ClinicalTrials.gov study NCT05234983. IPD Sharing: NO. Countries: 1. Publications: 3.

restrictedIPD-UNDECIDEDFeb 2026View details →

Tobacco Dependence in Breast Cancer Patients Trial of Varenicline (Chantix)

ClinicalTrials.gov study NCT01532232. IPD Sharing: Not stated. Countries: 1. Publications: 1.

ADHAirE : a Randomized Controlled Trial to Improve the Implementation of Tobacco-free School Policies

ClinicalTrials.gov study NCT06655038. IPD Sharing: NO. Countries: 1. Publications: 1.