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opencc-zeroJan 2024View details →

Supplementary material 4 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778

Cluster analysis (Ward's method) of size-corrected morphometric data of analyzed specimens of Diapoma pampeana

opennotspecifiedNov 2018View details →

Fig. 6. ITS2 secondary structures showing significant variations between our isolate A in Funiculosone, a substituted dihydroxanthene-1,9-dione with two of its analogues produced by an endolichenic fungus Talaromyces funiculosus and their antimicrobial activity

Fig. 6. ITS2 secondary structures showing significant variations between our isolate A. Talaromyces sp. (MF927596.1*) and B. T. funiculosus (consensus), incompatible base pairs are highlighted in pale red and yellow colour based on their degrees of incompatibility; * indicating own isolate. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 5. C2C12 in Insulin-mimetic activity of 23-glycosyl oleanane triterpenoids isolated from Gymnema latifolium

Fig. 5. C2C12 myotube cells were exposed to compounds 2 and 4 (5 μM and 20 μM, respectively) and incubated for 1 h. The phosphorylation of the protein AMPK in the cells was assessed by Western blot analysis. Fold expression was calculated as p-AMPK/AMPK and normalized to the protein level of β-actin. Values are expressed as the mean ± SD (n = 3).

Fig. 2. Key 1H–1H in Insulin-mimetic activity of 23-glycosyl oleanane triterpenoids isolated from Gymnema latifolium

Fig. 2. Key 1H–1H COSY (bold) and HMBC (1H → 13C, green arrows) correlations for compounds 1, 2, 5, and 7. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 4 in Insulin-mimetic activity of 23-glycosyl oleanane triterpenoids isolated from Gymnema latifolium

Fig. 4. Stimulatory effects of compounds 1⎯9 on glucose uptake in 3T3-L1 adipocytes using the fluorescent glucose derivative 2-NBDG. (A) Differentiated adipocytes were treated with isolated compounds (1–9) at a concentration of 20 μM and insulin (100 nM) as a positive control. After 1 h of incubation with or without 2-NBDG, images were captured by fluorescence microscopy. (B) The fluorescence signal in the adipocytes was measured and analyzed. After the cells were incubated for 1 h with or without 2-NBDG, the fluorescence signals were measured at Ex/Em = 450/535 nm. The results are presented as the mean ± SD (n = 3) of experiments performed in triplicate; *p <0.05, **p <0.01, and ***p <0.001, compared to the vehicle group. (C) Differentiated 3T3-L1 adipocytes were treated with compounds 2 and 4 at concentrations of 1, 5, and 20 μM or insulin at 100 nM. After 1 h of incubation, the fluorescence intensities were measured using a fluorescence microscope. (D) The concentration–response effects on glucose uptake in 3T3-L1 adipocytes. Cells were exposed to compounds at various concentrations (5, 10, and 20 μM) and incubated for 1 h. The cells were lysed, and the fluorescence signals were measured at Ex/Em =450/535 nm. Data are expressed as the mean ±SD (n =3) of experiments performed in triplicate; *p <0.05, **p <0.01, and ***p <0.001, compared to the negative control.

Fig. 5 in Isolation and functional characterization of an antifungal hydrophilic peptide, Skh-AMP1, derived from Satureja khuzistanica leaves

Fig. 5. The effects of temperature (a) and pH (b) on the stability of Skh-AMP1 as measured by its biological activity toward Aspergillus fumigatus growth. (c) Assay of Skh-AMP1cytotoxicity against HEK293 cells after 6, 24 and 48 h time periods. Results represent mean ± SD of three independent experiments.

Fig. 4 in Isolation and functional characterization of an antifungal hydrophilic peptide, Skh-AMP1, derived from Satureja khuzistanica leaves

Fig. 4. (a) Primary structure alignment of Skh-AMP1 against other antifungal peptides from different plant sources is shown. Colored residues correspond to the conserved residues. (b) Three-dimensional structure model of Skh-AMP1. Hydrophilic and hydrophobic areas are shown red and blue regions, respectively. (c) Helical wheel projection of Skh-AMP1. The hydrophilic residues as circles, hydrophobic residues as diamonds, potentially negatively charged as triangles, and potentially positively charged as pentagons.

Fig. 1 in Isolation and functional characterization of an antifungal hydrophilic peptide, Skh-AMP1, derived from Satureja khuzistanica leaves

Fig. 1. (a) Purification of peptides from Satureja khuzistanica leaves. Two hundred fifty μL aliquot of lyophilized extract subjected to reverse phase-HPLC column by using a gradient from 5 to 65% (v/v) solution B (0.098% TFA in acetonitrile) combined with solution A (0.1% TFA in water) during 85 min. The absorbance was monitored at wavelength 220 nm. Totally, 15 peaks were collected. (b) The purity peak S2 was evaluated using the same column and method. (c) Tricine-SDS-PAGE profile of (1) protein standards from 2 kDa to 250 kDa (2) peptide extract passed through an ultra membrane with a 10-kDa cutoff, (3) The peak S2 purified from RP-HPLC column.

Fig. 4 in Nine geranylgeranylated derivatives isolated from the roots of Rhus chinensis Mill.

Fig. 4. The gastroprotective effects of the 95% EtOH extract (95% EXT) and 95% EtOH fraction from a microporous resin (95% FRA) of the roots of R. chinensis (R.C.) against indomethacin-induced (A, macroscopic observation; B, graphical representation) and water immersion-restraint stress-induced (C, macroscopic observation; D, graphical representation) gastric ulceration (with pantoprazole sodium as the positive control, ***p <0.05, n = 5).

Fig. 3 in Nine geranylgeranylated derivatives isolated from the roots of Rhus chinensis Mill.

Fig. 3. Chiral HPLC-UV chromatogram of racemic compounds 6 (6a and 6b) (A) and 7 (7a and 7b) (B) using a Chiralpak AD-H column (4.6 mm × 250 mm, 5 μm), isocratic elution with n-hexane/EtOH (98:2, v/v) or (90:10, v/v), respectively, flow rate 0.7 mL/min, UV detection at 254 nm, temperature at 25 ◦ C; ECD spectra for enantiomers of 6 (6a and 6b) (C) and 7 (7a and 7b) (D).

Fig. 3 in Isolation and functional characterization of an antifungal hydrophilic peptide, Skh-AMP1, derived from Satureja khuzistanica leaves

Fig. 3. Antifungal effects of peak S2 (identified as Skh-AMP1) against Candida albicans (1), Candida krusei (2), Candida glabrata (3), Aspergillus niger (4), Aspergillus flavus (5) and Aspergillus fumigatus (6) are shown. Amphotericin B and Peak S2 were used at 17.31 μM and 36 μM concentrations, respectively. Results represent means ± SD of three independent experiments.

Fig. 2 in Isolation and functional characterization of an antifungal hydrophilic peptide, Skh-AMP1, derived from Satureja khuzistanica leaves

Fig. 2. (a) Mass spectrum of peptide showing multiply protonated ions of the analyte. Note that not the first (monoisotopic) signals are annotated, but the most abundant one where 13C is incorporated. (b) MS/MS spectrum of peptide QELCTWERGSVRQADK (LysC digest, coverage from doubly protonated spectrum in top of list, from triply protonated ion in bottom of list). (c). MS/MS spectrum profile of a) peptide TSKQELCTWER (tryptic digest), b) peptide GSVRQADKTLAG (tryptic digest), c) peptide GRTSKQELCTWER (tryptic digest).

restrictedcc-by-4.0Dec 2023View details →

Data from: Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture.

Data from  Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. Files in .JBN and .xlsx format

opencc-by-4.0Mar 2024View details →

Comparative membrane proteomic analysis of Tritrichomonas foetus isolates (non filtered Data Sets)

Tritrichomonas foetus is a flagellated and anaerobic parasite able to infect cattle and felines. Despite its prevalence, there is no effective standardized or legal treatment for T. foetus-infected cattle; the vaccination still has limited success in mitigating infections and reducing abortion risk; and nowadays, the diagnosis of T. foetus presents important limitations in terms of sensitivity and specificity in bovines. Here, we characterize the plasma membrane proteome of T. foetus and identify proteins that are represented in different isolates of this protozoan.  Raw proteomics data sets from MALDI-TOF Mass Spectrometry presented here corresponds to six T. foetus isolates (Tf0-Tf5). For Tf2 isolate also five membrane fractions are presented (f1-f5).

opencc-by-4.0Apr 2017View details →

Isolation of a novel Streptococcus anginosus subspecies, AW1 from wound infection in Al-Madinah Al-Munawarah province

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opencc-by-4.0Nov 2024View details →

Draft Genome Sequences of 38 Aspergillus parasiticus isolates

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dryad32/100

Isolating human monoclonal antibodies against adeno-associated virus from donors with pre-existing immunity

With the advent of single B-cell cloning technology, we can isolate antibodies against virtually any antigen to study the interaction of a given pathogen with the immune system and develop novel therapeutic strategies. Antibodies directed against the capsid of adeno-associated viruses (AAV) are a significant obstacle to effectively leveraging AAV as a gene-delivery vector in seropositive individuals. In order to design next-generation vectors that can evade neutralization by these antibodies, studies have mapped the epitopes of mousemonoclonal antibodies generated by immunization with AAV. Although these studies provide critical information regarding capsid immunogenicity, they cannot address (1) differences in the antibody repertoire generated in humans following AAV natural infection; or (2) how reactions can vary when generated in response to vector administration. Here, we isolated and evaluated a panel of novel, fully human anti-AAV antibodies by cloning single memory B cells from a seropositive normal donor. We have validated the utility of this approach to study AAV immunology. Our goal is to leverage this knowledge to design novel AAV variants that can effectively transduce target tissues in individuals with AAV-neutralizing antibodies.

opencc-zeroOct 2021View details →

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Isolation and characterization of twelve polymorphic microsatellite markers in the endangered Hopea hainanensis (Dipterocarpaceae)

Using next-generation sequencing technology, 26 microsatellite markers were developed and genotyped by capillary electrophoresis for Hopea hainanensis Merrill & Chun, an endangered tree species in Hainan Island and northern Vietnam. Twelve markers were found to be polymorphic in this species. Primer transferability was tested with H. chinensis Hand.-Mazz. and H. reticulata Tardieu, in which 3 and 7 microsatellite markers were found to be polymorphic, separately. The 12 polymorphic microsatellite markers could be applied to population genetic studies aimed at H. hainanensis as well as its close relatives, facilitating the conservation and restoration of these endangered but valuable Hopea species.

opencc-zeroNov 2021View details →