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ShareScore release 0.9.0
Dataset results
24 results for “assay optimization”
Tandem Tag Assay Optimized for Semi-automated in vivo Autophagic Activity Measurement in Arabidopsis thaliana roots
<p>This is demo data set for AuTToFlux, the semi-automated assay for <em>in vivo</em> autophagic activity measurement in <em>Arabidopsis thaliana</em> roots.</p> <p>The protocol and software required for the assay are available here: <a href="https://github.com/jonasoh/AuTToFlux">https://github.com/jonasoh/AuTToFlux</a></p>
Development and Optimization of an IQF USP5 Activity Assay
<p>Development and optimization of a fluorescence based deubiquitinase (DUB) activity assay for USP5 with internally quenched fluorophore pair (IQF) substrates: di-ubiquitin lysine linked K48 and K63 (Ub2K48, Ub2K63). The inhibitory capability of USP5 zinc-finger ubiquitin binding domain (ZnF-UBD) ligands was investigated. </p>
Optimization and evaluation of a two-stage chromogenic tenase assay for measurement of emicizumab plasma levels
<p>A two-stage chromogenic tenase assay allows specific measurement of emicizumab plasma levels over a broad concentration range (10 µg/ml to 100 µg/ml). The assay showed a reasonable agreement with one-stage clotting assy used for emicizumab quantificaition and can be applied on an automated coagulation analyzer, demonstrating its applicability within a routine laboratory setting.</p>
ROS-specific Huntingtin Interactions: GFP reactivation assay optimization in HEK 293 cells
<p>Optimization step in the lead up to phenotypic analysis of ROS-dependent huntingtin interacting proteins.</p>
USP5 Zf-UBD Fluorescence Polarization Displacement Assay Optimization
<p>Optimization of a fluorescence polarization (FP) assay for USP5 zinc finger ubiquitin binding domain (Zf-UBD) with an N-terminal FITC-labeled ubiquitin RLRGG peptide. </p>
USP5 Zf-UBD Differential Scanning Fluorimetry Assay Optimization
<p>Optimization of a differential scanning fluorimetry (DSF) assay for USP5 zinc finger ubiquitin binding domain (Zf-UBD) to determine if the presence of detergent in buffer conditions results in significant difference in melting temperatures of USP5 Zf-UBD and if the addition of increasing concentrations of ubiquitin RLRGG peptide results in thermal shift of USP5 Zf-UBD. </p>
The process of HDAC11 Assay Development: Buffer optimization again
<p>The pH range used earlier was expanded for further optimization to check if the activity could improve.</p> <p> </p> <p><strong>Note: </strong>1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).</p> <p> 2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.</p>
Randomized Trial to Optimize Virologic Suppression Rates Using a Point-of-Care Urine Monitoring Assay (ROVING PUMA)
ClinicalTrials.gov study NCT06423612. IPD Sharing: YES. Countries: 1. Publications: 6.
Optimizing the Care Pathway of Febrile Children Via Capillary C-reactive Protein Assay in Primary Care
ClinicalTrials.gov study NCT06910631. IPD Sharing: YES. Countries: 1. Publications: 1.
Data from: A second-generation diagnostic single nucleotide polymorphism (SNP)-based assay, optimized to distinguish among eight poplar (Populus L.) species and their early hybrids
Open the record for dataset details and reuse information.
FACS validation dataset: An optimized library for reference-based deconvolution of whole-blood biospecimens assayed using the Illumina HumanMethylationEPIC BeadArray (III)
GEO Series GSE112618. Homo sapiens. 6 samples. Type: Methylation profiling by genome tiling array.
Pilot testing and optimization of a larval fathead minnow high throughput transcriptomics assay
GEO Series GSE207231. Pimephales promelas. 878 samples. Type: Expression profiling by high throughput sequencing.
Systematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay
GEO Series GSE31982. Escherichia coli; Homo sapiens. 18 samples. Type: Expression profiling by high throughput sequencing.
Optimization of the TeraTox assay for preclinical teratogenicity assessment
GEO Series GSE183534. Homo sapiens. 1055 samples. Type: Expression profiling by high throughput sequencing.
FlowSorted.Blood.EPIC: An optimized library for reference-based deconvolution of whole-blood biospecimens assayed using the Illumina HumanMethylationEPIC BeadArray (II)
GEO Series GSE110554. Homo sapiens. 49 samples. Type: Methylation profiling by genome tiling array.
A multimodal assay identifies optimized binders for a bicistronic CAR against GPC2 OR CD276 to overcome heterogeneity in Neuroblastoma
GEO Series GSE181437. Homo sapiens. 15 samples. Type: Expression profiling by high throughput sequencing.
Longitudinal dataset: An optimized library for reference-based deconvolution of whole-blood biospecimens assayed using the Illumina HumanMethylationEPIC BeadArray (I)
GEO Series GSE110530. Homo sapiens. 12 samples. Type: Methylation profiling by genome tiling array.
Optimization of Human Neural Progenitor Cells for an Imaging-Based High-Throughput Phenotypic Profiling Assay for Developmental Neurotoxicity Screening
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Sample Collection From Healthy Volunteers for Assay Optimization
ClinicalTrials.gov study NCT03538600. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Noninvasive Screening for Affected Pregnancies: Assay Development & Optimization in Affected Pregnancies
ClinicalTrials.gov study NCT01052688. IPD Sharing: NO. Countries: 1. Publications: 0.
ScienceDex guides
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.