Open Field Test citations

The apparatus consisted of an open field (40.5 × 40.5 × 30 cm) composed entirely of solid, light gray plastic (Maze Engineers, Glenview, IL, USA).

For the open field test, mice were placed in the corner of an open field apparatus (40 × 40 × 40 cm; Maze Engineers) and allowed to freely explore for 5 min under dim white indirect light (Smith et al., 2023).

...blue open-field chambers (40 × 40 × 40 cm; MazeEngineers, USA) used for the closed-loop optostimulation experiment.

Open field maze (OF) and novel object recognition (NOR) behavioural assessments were performed in this study using mazes from Maze Engineers (60077, Skokie, Illinois, USA).

For testing, each animal was placed in the corner of a 60 × 60 cm × 40 cm arena (Maze Engineers, Skokie, IL).

The OF apparatus consisted of a beige ABS-plastic arena (40 cm × 40 cm × 30 cm; 3201 mouse open field, Maze Engineers Inc., Boston, MA, USA).

Mice were placed in the center of a chamber measuring 40 cm (length) × 40 cm (width) × 40 cm (height) (Maze Engineers) and allowed to explore the chamber for 5 min freely (500 lx throughout the chamber).

All the behavioural assays were performed in the same chambers (40 × 40 cm, MazeEngineers).

Locomotor activity, exploration behavior, and anxiety-like behavior were assessed using the open-field test (Maze Engineers, Skokie, IL, USA)

On testing day, a single animal was transported to the testing room in a clean transport cage and placed in the corner of a 100 × 100 cm × 40 cm open field (Maze Engineers, Skokie, IL).

A total of n = 11 CRHTRA1 and n = 13 WT rats aged to 12 M were used. The open field (Maze Engineers, Skokie, IL) measures 60 cm x 60 cm and is constructed with matte black acrylic floor and walls.

...required, as many commercial arenas are available, for example, from Maze Engineers (https://maze.conductscience.com).

General locomotor behavior of mice was evaluated in multi-unit open field mazes (Maze Engineers).

∼6 days after the catheter implantation, mice were placed in a Black Plexiglas testing chamber (Maze Engineers; 60 cm for both side-widths x 50 cm in height with a floor illumination at ∼60 lux) (Shan et al., 2019) (Fig. 1A).

The apparatus used was an open-field box (50 × 50 cm, Maze Engineers, Catalog #3201).

Mice were then placed in an open field box with dimensions 40 × 40 × 40 cm (Maze Engineers, Boston, MA) for 10 minutes at 60 lux.

...were placed in the center of a chamber measured 40 cm (length) × 40 cm (width) × 40 cm (height) (Maze Engineers) and allowed to explore the chamber for 5 min freely (750 lux throughout the chamber).

A rodent open field apparatus (Maze engineers, part# 3203) was used for this assay.

...in the corner of an evenly lit square arena (80 cm × 80 cm, 41 cm height) without visual cues (Maze Engineers; Skokie, IL), and average velocity, track length, activity level, and time spent in the central...

The open field box was made with a black acrylic material, with a dimension of 60 cm (length) × 60 cm (width) × 40 cm (height) (Maze engineers, USA).

In each experiment, a testing mouse was placed in a squared open chamber at the dimensions of 40 (l) x 40 (w) x 30 (h) cm (MazeEngineer).

...Briefly, mice were placed in the center of an empty testing area (40 × 40 cm; 30cm height, Maze Engineers) and allowed to move freely for 10 min.

TOR was conducted in an 80 × 80 × 30 cm white acrylic open field arena, with white opaque wall inserts separating the arena into (4) 40 × 40 × 30 cm height square chambers (Conduct Science).

Briefly, mice were allowed to explore a 40 × 40 cm arena (Maze Engineers, # 3201) illuminated at 60 l × for 5 min during which the distance traveled and the time spent in the inner 15 cm-area were measured using the EthoVision tracking system (XT 17, Noldus Information Technology).

The OFT was conducted in a 100cm x 100cm black plexiglass square arena with 40cm tall walls (Maze Engineers).

A gray open field box purchased from Maze Engineers was used for habituation and for both trials.

To quantify the effect of drug on locomotion, mice were placed in a 40 × 40 × 30-cm box (Maze Engineers) and allowed to freely explore.

An open-field arena (a square, empty arena, 60 cm × 60 cm surrounded by walls 40 cm in height, Maze Engineers, Illinois) was used to assess anxietylike behavior in this study (Seibenhener and Wooten, 2015).

Mice were habituated to open field arenas (40×40×40cm; MazeEngineers, USA) for 30 minutes on the first day and 15 minutes on the second day.

All the behavioral assays were performed in the same chambers (40 × 40 cm, MazeEngineers).

...consists of an open field (40.5 × 40.5 × 30cm) composed entirely of solid, light gray plastic (Maze Engineers, Glenview, IL, USA).

...behavior, and was performed in a standardized arena of 40 × 40 cm made from inert plastic (Maze Engineers, Skokie, IL, USA).

...toward an unfamiliar CD1 target animal. The arena was a white open field (40 × 40 cm height; Maze Engineers) maintained in complete darkness supplemented with red lamp lights.

...were allowed to explore four novel objects in a square open field box purchased from Maze Engineers (40cm long, 40cm wide, 30cm tall) for 7 minutes (mice not exposed to the novel objects were used...

...field test consisted of 5 min trial in a white opaque arena of 45 cm × 45 cm × 30 cm in size (Maze Engineers, Boston, USA). The test arena was divided equally into nine square quadrants.

The OFT/OLT arena (Maze engineers, 412 Wilmette Ave, Glenview, IL 60025, USA) (80 × 80 × 30 cm) had four opaque plexiglass compartments (40 × 40 × 30 cm) that allowed us to test the four pups per litter at the same time (Fig. 6a).

...were assessed using an open field arena [60 cm × 60 cm surrounded by walls 40 cm in height (Maze Engineers, IL)].

...plastic open field box with dimensions of 40cm wide, 40cm long, and a 30cm height was purchased (Maze Engineers), and the box surrounded by distal spatial cues.

The apparatus consists of an open field (40.5 × 40.5 × 30cm) composed entirely of solid, light grey plastic (Maze Engineers, Glenview, IL, USA).

Mice were placed in an open field box with dimensions 40 × 40 × 40 cm (Maze Engineers, Boston, MA).

...(Grn+/+). The test consisted of one 10 min trial in a white opaque 40 cm × 40 cm × 30 cm arena (Maze Engineers).

The test chamber was 60 cm for both side-widths × 40cm in height (Maze Engineers) with a floor illumination at ~60–80 lux.

...The recording arena consisted of a 46 × 46 × 55 cm gray acrylic box with an open top (Maze Engineers)...

Mice were allowed to explore a 40 × 40 × 40 cm box (MazeEngineers, United States) for 5 min.

...consists of a square field (72 X 72 cm) surrounded by outer walls with a height of 25-28 cm (Maze Engineers; Boston, MA).