Primary Assay — Mouse
Three-Chamber Social Interaction
Mus musculus
Assesses sociability and social novelty preference. Mouse chooses between a chamber containing a stranger mouse vs an empty chamber (sociability), then between a familiar vs novel stranger (social novelty).
Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Mus musculus.
| Parameter | Unit | Description |
|---|---|---|
| Time near stranger vs empty | s | Sociability index |
| Time near novel vs familiar | s | Social novelty preference |
| Approach frequency | count | Number of entries to interaction zones |
| Sniffing time | s | Direct investigation of stimulus mouse |
| Social preference index | ratio | (Stranger − Empty) / (Stranger + Empty) |
References
Citations for Three-Chamber Social Interaction
- Moy SS, et al. (2004). Sociability and preference for social novelty in five inbred strains: an approach to assess autistic-like behavior in mice. Genes Brain Behav, 3(5), 287-302. PMID: 15344922
- Crawley JN. (2004). Designing mouse behavioral tasks relevant to autistic-like behaviors. Ment Retard Dev Disabil Res Rev, 10(4), 248-258. PMID: 15666338
- Kaidanovich-Beilin O, et al. (2011). Assessment of social interaction behaviors. J Vis Exp, (48), 2473. PMID: 21403628
Compatible Equipment
Hardware for Mouse Research
Open Field Maze (various sizes)
OFT locomotion and anxiety
Elevated Plus Maze
Anxiety-like behavior
Morris Water Maze
Spatial learning and memory
Fear Conditioning Chamber
Contextual and cued FC
Rotarod (accelerating)
Motor coordination
Three-Chamber Social Interaction
Sociability testing
Y-Maze
Spontaneous alternation
Barnes Maze
Spatial learning (dry, less stressful)
Light-Dark Box
Anxiety-like behavior
Forced Swim Test Cylinder
Depressive-like behavior
Tail Suspension Apparatus
Depressive-like behavior
Grip Strength Meter
Neuromuscular function
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Open Field Test
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Elevated Plus Maze (EPM)
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Morris Water Maze
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Run Three-Chamber Social Interaction on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your mouse facility.
