Tracked Object · Nuclei

Nuclei Tracking

Track the nucleus when the cytoplasm is invisible.

Nuclei are one of the four object classes ConductVision Image tracks. With an H2B-GFP or Hoechst label and no cytoplasmic stain, the engine segments and links each nucleus frame by frame, giving you the same trajectory, division, and lineage readouts as whole-cell tracking, plus mitosis timing straight from the division tree.

Fluorescence micrograph: a field of DAPI-stained cell nuclei glowing blue on a black background, the kind of labeled-nuclei image the engine segments and links frame by frame.
DAPI-stained cell nuclei — Accuratelibrarian, CC BY-SA 4.0, via Wikimedia Commons
Nuclei
Tracked object class
Frame-by-frame
Trajectory + lineage
Mitosis
Division timing
Outputs

What gets measured

Nuclei run through the same segment → link → lineage → analysis pipeline as cells, so every tracking table applies unchanged.

01

Motion (exported)

  • TotalPathLength
  • NetDisplacement
  • Directionality
02

Lineage (exported)

  • IsDivision
  • Children
  • Depth
  • RootId
  • IsCensored
03

Temporal (exported)

  • AvgSpeed
  • ProliferationRate
  • SurvivalRate
04

Population (exported)

  • TreeCount
  • BranchingRate
  • MeanLifeSec
  • LeafFraction
Workflow

How It Works

1

Load video

Point a tracking protocol at a time-lapse of labeled nuclei.

2

Set nuclear diameter

Draw one average nucleus so the model calibrates to your imaging scale.

3

Track & link

AI detects each nucleus and links it across frames, flagging divisions.

4

Export

Download motion, lineage, temporal, and population tables as CSV.

Ready to track nuclei in your own time-lapse?

Request a demo and see ConductVision Image link and score your nuclei frame by frame.

Request a Demo