Kidney structures
ConductVision Image finds every glomerulus in your kidney sections and measures its size, mesangial matrix, sclerosis, crescents and podocytes on the stains your lab uses.
Scale
As the view moves through the glomerulus, ConductVision Image keeps a box, an outline and a label on each cell it tracks. Choose a cell on the field or under Tracking to follow it.
Structures
Each card pairs a published photo of a structure with the measures ConductVision Image takes on it and the stains they are read on. Pick a stain to see only the structures it shows.
All 5 structures are shown.
Open capillary loops fill the tuft, with a thin core of mesangial matrix between them.
Measures
Read on
Matrix replaces the capillary loops, in part of the tuft or across all of it.
Measures
Read on
Mesangial matrix widens between the loops, and the open spaces in the tuft narrow.
Measures
Read on
Layers of cells fill Bowman's space and press on the tuft from the outside.
Measures
Read on
Podocytes wrap the outside of the capillary loops, and a marker such as WT1 stains their nuclei.
Measures
Read on
Measures
Every measure becomes a column in the summary tables ConductVision Image exports as CSV files, in the table for each section and, for most, the table for each animal.
| Measure | Unit | Read on | Exported as |
|---|---|---|---|
| Glomerular density | Unitglomeruli per mm² of cortex | Read onEvery stain | Exported asGlomerular density, per section and per animal |
| Tuft area | Unitµm² | Read onEvery stain | Exported asMean tuft area, per section and per animal |
| Bowman's capsule area | Unitµm² | Read onEvery stain | Exported asMean capsule area, per section |
| Mesangial matrix area | Unitpercent of the tuft | Read on
| Exported asMesangial matrix area, per section and per animal |
| Sclerosed area | Unitpercent of the tuft | Read on
| Exported asSclerosed area, per section |
| Glomerulosclerosis score | Unitscore for each glomerulus | Read on
| Exported asGlomerulosclerosis index, per section and per animal |
| Crescents | Unitpresent or absent in each glomerulus | Read on
| Exported asCrescents, per section and per animal |
| Podocytes per glomerulus | UnitWT1 or p57 positive nuclei | Read on
| Exported asPodocytes per glomerulus, per section and per animal |
| IHC positive area | Unitpercent of the structure | Read on
| Exported asIHC positive area, per section and per animal |
Stains
Glomeruli are found and sized on every stain. Each stain then adds the measures listed beside it.
| Stain | What it shows in glomeruli | ConductVision Image measures |
|---|---|---|
| PAS | What it shows in glomeruliMesangial matrix and capillary basement membranes stain magenta, so matrix growth, sclerosis and crescents stand out. | ConductVision Image measures
|
| H&E | What it shows in glomeruliNuclei and cells stand out, so crescents and crowded tufts are easy to see. | ConductVision Image measures
|
| Masson's trichrome | What it shows in glomeruliCollagen in a scarred tuft stains blue, while cell cytoplasm stains red. | ConductVision Image measures
|
| Picrosirius red | What it shows in glomeruliCollagen stains red, so scarring around Bowman's capsule stands out. | ConductVision Image measures
|
| IHC | What it shows in glomeruliA marker shows brown in the cells it labels, such as WT1 in podocyte nuclei. | ConductVision Image measures
|
Study planner
Enter your study design to see the animals and slides it covers. The planner opens on PAS and one IHC marker, which together give every measure on this page.
Other model, 4 groups of 8 animals at 1 time point (32 animals). PAS and 1 IHC marker, with 1 section per stain (64 slides).
Measures from these stains
The pilot form opens with this plan attached, and you can add details before you send it.
No. ConductVision Image is for research use only and is not for diagnosis. Labs use it on glomeruli in sections from animal studies and in human tissue held for research.
Yes. ConductVision Image finds and outlines every glomerulus in the cortex it measures, leaving out flagged areas such as folds. Glomerular density is that count divided by the cortex area.
ConductVision Image measures the sclerosed share of each tuft on PAS and gives each glomerulus a glomerulosclerosis score from it. The scores average into an index for each section and each animal.
Every stain on this page works for finding glomeruli and measuring their size. PAS adds mesangial matrix, sclerosis and crescents, and IHC adds podocyte counts and positive area.
Yes. On IHC for a podocyte marker such as WT1 or p57, ConductVision Image counts the positive nuclei inside each glomerulus and reports podocytes per glomerulus.
Yes. ConductVision Image exports every measure in CSV tables, so you can line its results up with the scores your lab already keeps.
You send a few stained sections that span your study, and our team measures their glomeruli with the measures you choose. You then review the tables and decide whether to measure the full study.
Tell us about your study, and we will plan a pilot on a few of its sections.
