Behavioral Mazes

Activity Cage

SKU ME-5607
$2,990.00
IncludesStandard care · Standard delivery

Automated rodent activity monitoring system with integrated running wheel, capacitive touch screen control, and comprehensive data recording for locomotor and circadian rhythm studies.

Scientist guidance
Louise Corscadden, PhD, Director of Science

Louise Corscadden, PhD

Director of Science · ConductScience

Ask Louise about Activity Cage fit, setup, configuration, or quote prep.

Key Specifications

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Model fit
Mouse, Rat
SKU family
ME-5607
Sizing
43.2 x 38.0 x 27.9 cm
Ordering
Online checkout and quote request available
Category
Behavioral Mazes
Build notes
Stainless Steel
Category: Behavioral Mazes
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Frequently Bought Together

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The complete Activity Cage workflow

Track behavior

No exact ConductVision activity-cage page is currently published. Locomotor counts are captured by infrared beam breaks rather than overhead tracking, so automated video scoring of cage activity stays a roadmap gap.

Supporting page not yet built

Run protocol

No exact ConductMaze activity-cage protocol is currently published. Habituation, session length, beam-spacing setup, and circadian-timing routines are apparatus-specific; keep this as a roadmap gap.

Supporting page not yet built

Analyze output

No exact activity-cage analysis tool is currently published. Distance, ambulatory and rearing counts, and habituation slope are summarized from beam-break logs rather than a dedicated analyzer; keep this as a roadmap gap.

Supporting page not yet built

Configuration considerations

Common Activity Cage setup decisions

Use these notes to scope species, cohort, tracking, and automation needs. Only verified product or support routes are linked from this section.

This productInfrared-beam

Activity Cage

Enclosed cage with horizontal infrared beam grid and a software counter for ambulatory and stereotypic counts

Standard configuration for automated locomotor monitoring, reporting total distance and ambulatory counts from horizontal beam breaks over a fixed session.

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BuyableMouse or rat

Species-Scaled Activity Cage

Cage footprint and beam spacing scaled for mouse or rat body size

Beam spacing relative to body size sets the spatial resolution of the counts, so the cage and grid should match the species being recorded.

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SpecialtyVertical beams

Dual-Plane / Rearing Activity Cage

Horizontal plus vertical beam planes for ambulatory and rearing counts

Best when vertical exploration matters, with a second beam plane that separates rearing from horizontal ambulation rather than collapsing them into one count.

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§ 1

Introduction

The Activity Cage measures spontaneous locomotor activity by counting infrared beam breaks as a rodent moves through an enclosed cage. Automated activity monitoring turns open-field-style locomotion into a continuous, observer-independent record of distance, ambulation, and rearing. 1

The core readouts are total distance, ambulatory counts, rearing or vertical counts, and the distribution of activity between the cage center and walls. Because beam-break monitoring runs unattended, it is widely used to phenotype hyperactivity, sedation, and drug effects, and to track activity across long circadian windows. 1

Novelty and habituation, circadian timing, handling, beam spacing, and whether animals are tested alone or in groups all change activity counts independent of the manipulation. A defensible protocol fixes the session length, controls the time of day, allows for within-session habituation, and reports beam resolution so counts are comparable across studies. 1

§ 2

Methods

2.1 Procedure

Beam-break locomotor monitoring with habituation handling, circadian control, and resolution-aware count scoring.

Pre-test setup

  1. 1.Acclimation and handlingAcclimate animals to the room and to handling so the first session reflects baseline locomotion rather than novelty stress and handling arousal.
  2. 2.Beam calibrationVerify horizontal and, where present, vertical beam alignment and spacing, and confirm the counter distinguishes ambulatory from stereotypic breaks.
  3. 3.Fix the sessionSet the session length, light level, and time of day in advance, since activity falls within a session as animals habituate and varies across the circadian cycle.
  4. 4.Define count rulesPre-define ambulatory versus stereotypic counts and the center-versus-periphery boundary so anxiety-like and locomotor measures are scored consistently.

Trial sequence

  1. 1.Place and startPlace the animal in the cage and start the counter, recording from the first beam break so the within-session habituation curve is captured.
  2. 2.Record ambulationLog ambulatory counts and total distance from horizontal beam breaks over the fixed session.3
  3. 3.Count rearingRecord vertical beam breaks as rearing counts where a second plane is present, since rearing indexes exploration distinctly from horizontal movement.1
  4. 4.Track center activityLog activity in the center versus the periphery, since center avoidance is an anxiety-like measure that rides alongside locomotion.2
  5. 5.Clean between subjectsClean the cage and beam frame between animals to remove odor and urine cues that alter exploration and thigmotaxis.

Critical methodological constraints

  • Novelty and habituation. Activity is highest at the start and falls as the animal habituates. Report the within-session slope rather than a single total, and allow a habituation window where appropriate.2
  • Circadian timing. Rodent activity varies strongly across the light-dark cycle. Fix the time of day across groups, or record across full circadian windows.5
  • Beam resolution. Beam spacing sets the smallest movement that registers. Coarse grids miss fine movements, so resolution must be reported and held constant for comparison.3
  • Stereotypy versus ambulation. Repetitive movement in one location inflates raw counts. Separate ambulatory from stereotypic counts so locomotion is not overstated.

2.2 Measurement & Analysis

Core activity-cage endpoints for locomotion, exploration, and within-session quality control.

Total Distance

Locomotion

Path length reconstructed from sequential beam breaks, the primary index of overall locomotor activity.3

Ambulatory Counts

Horizontal activity

Horizontal beam breaks reflecting whole-body movement, separated from repetitive stereotypic breaks.

Rearing/Vertical Counts

Exploration

Vertical beam breaks indexing rearing and exploratory posture, recorded where a second beam plane is present.1

Center Activity

Anxiety-like confound

Activity in the center versus the periphery, an anxiety-like thigmotaxis measure that accompanies locomotor counts.2

Habituation Slope

Within-session adaptation

Decline in activity across the session as the animal habituates, separating novelty-driven activity from steady-state locomotion.

+ Additional metrics: stereotypic counts, time of day, session length, beam spacing, resting time, and per-session apparatus notes.

2.3 ambulatory fraction (analysis)

A compact fraction of total beam breaks attributable to ambulation rather than stereotypy.

Inline calculator

Type the values your tracker recorded.

Full calculator with 95% CI ->
Ambulatory fraction

70.0%

Formula: ambulatory counts / (ambulatory counts + stereotypic counts) x 100. Interpret with the habituation slope, circadian timing, beam resolution, and center activity because a high fraction can still reflect novelty-driven activity rather than steady-state locomotion. 1

2.4 sample-size planning

Estimate the N per group needed to detect a literature-anchored locomotor effect at the endpoint you plan to report. Override the defaults with your own pilot numbers.

sample-size planning

Estimate the N per group needed to detect a literature-anchored locomotor effect at the endpoint you plan to report. Override the defaults with your own pilot numbers.

Control vs hyperactive mouse in an activity monitor; representative magnitudes from Tatem et al. (2014) on open-field activity monitoring.3

Cohen's d

1.69

N per group at 80% power

6

Total N

12

With attrition cushion

14

At 70% / 90% power

5 / 8

Methods sentence

Need ANOVA, proportions, paired design, or a power curve? Open in the full Sample-Size Calculator →

Formula: n = 2 · ((zα/2 + zβ) / d)2, where d = |μ₁ − μ₂| / σ. Assumes equal allocation, normality, and homoskedasticity. The attrition cushion inflates total N by 1 / (1 − dropout); confirm with your IACUC.

§ 3

Results

Aggregate publication data, sample apparatus output, and recent findings from the live PubMed feed.

3.1 Publication trends

PubMed volume and co-occurring behavioral methods for locomotor-activity monitoring studies.

Figure 1 · EPM publications by year (PubMed)

The paradigm has been dominant for 40 years and is still growing.

Live · Weekly

2000201020202025 YTD: 162 papers

Total in PubMed since 1985: 4,180+ papers. Updated 2026-06-12.

Figure 2 · Methods co-occurring with EPM (last 12 months)

Other paradigms most often run alongside EPM in the same paper.

Live

3.2 Sample apparatus output

Representative output from a 30-minute activity-cage session with horizontal and vertical beam planes.

Table 1 · Per-animal EPM scoring output

Download sample CSV →
AnimalGroupDistanceAmbulatoryRearingAmbulatory fraction
AC-001Control44 m6905869.7%
AC-002Control47 m7126271.2%
AC-003Control43 m6755568.9%
AC-004Hyperactive71 m10804078.3%
AC-005Hyperactive74 m11253779.0%
AC-006Hyperactive69 m10424277.6%

Synthetic example for illustration only. Pair distance with the habituation slope, circadian timing, and center activity before interpreting locomotor differences.

3.3 Recent findings (live PubMed feed)

  • Jun 2026Source note

    Locomotor-monitoring methods emphasize habituation-slope reporting over single totals.

    Static methods note aligned with Walsh & Cummins (1976), Prut & Belzung (2003), and Tatem et al. (2014).

    Activity peaks on novelty and declines with habituation, so report the within-session slope, fix the time of day, and document beam resolution before comparing locomotor counts across groups.

    Methods overviewReproducibility
  • Jun 2026Source note

    Beam resolution and circadian timing as comparability constraints.

    Static methods note aligned with Tang et al. (2002) and Crawley (1999).

    Count magnitudes are not comparable across grids of different beam spacing or across circadian phases. Report beam resolution, fix the time of day, and separate ambulatory from stereotypic counts.

    ResolutionCircadian timing

View all 4180matching papers on PubMed ->

§ 4

Discussion

Limitations of the paradigm, methodological caveats, and current directions.

4.1 Common confounds

Variables that shift Activity Cage results independent of anxiety state.

Novelty/habituation

Activity peaks on first exposure and falls as the animal habituates within and across sessions. Report the habituation slope rather than a single total count.

Circadian timing

Rodent locomotion varies strongly with the light-dark cycle. Fix the time of day across groups or record across full circadian windows.

Handling

Pre-test handling arouses animals and inflates early activity. Standardize handling and transfer so arousal does not differ between groups.

Beam spacing/resolution

Coarse beam grids miss fine movements and undercount distance. Report beam spacing and hold it constant for any cross-study comparison.

Individual vs group testing

Group-tested animals stimulate each other's movement. Whether animals are recorded alone or together must be fixed and reported.

Confound checklist

Tick the confounds your protocol addresses, then export a methods-paragraph blurb you can paste into your manuscript.

Preview exported markdown
## Activity Cage — methods controls

Confounds controlled in this protocol:

- **Novelty/habituation.** Activity peaks on first exposure and falls as the animal habituates within and across sessions. Report the habituation slope rather than a single total count.
- **Circadian timing.** Rodent locomotion varies strongly with the light-dark cycle. Fix the time of day across groups or record across full circadian windows.
- **Handling.** Pre-test handling arouses animals and inflates early activity. Standardize handling and transfer so arousal does not differ between groups.
- **Beam spacing/resolution.** Coarse beam grids miss fine movements and undercount distance. Report beam spacing and hold it constant for any cross-study comparison.
- **Individual vs group testing.** Group-tested animals stimulate each other's movement. Whether animals are recorded alone or together must be fixed and reported.

4.2 Construct validity caveats

Activity-cage counts are strongest when session length, time of day, beam resolution, and the ambulatory-versus-stereotypic distinction are fixed before testing. A single total count confounds novelty with steady-state locomotion; report the habituation slope and center activity, and confirm locomotor phenotypes with an independent open-field or home-cage measure. 1

4.3 Special considerations

How long should a session be?

Long enough to capture the within-session habituation curve, since a short session can sit entirely in the novelty phase. Fix the length in advance and report the activity slope rather than only a total.

Does beam spacing matter?

Yes. Beam spacing sets the smallest movement that registers, so coarse grids undercount distance and fine movements. Report the resolution and keep it constant across all groups in a study.

Should I separate ambulation from stereotypy?

Yes. Repetitive movement in one place inflates raw counts. Scoring ambulatory and stereotypic breaks separately keeps locomotion from being overstated by stationary repetitive behavior.

4.4 Current directions

Quarterly editorial review of emerging Activity Cage methodology. Q2 2026

Methods

Habituation-slope reporting

Reporting the within-session activity decline rather than a single total separates novelty-driven activity from steady-state locomotion and improves comparability.

Emerging

Long-window home-cage monitoring

Extended beam-break recording across full light-dark cycles captures circadian structure that a short novel-cage session cannot.

Methods

Resolution standardization

Reporting beam spacing and resolution is increasingly expected because count magnitudes are not comparable across grids of different density.

Emerging

Multi-plane activity decomposition

Adding vertical beam planes separates rearing from horizontal ambulation, giving distinct exploration and locomotion readouts from one session.

§ 5

References

5 selected methods and validation references for Activity Cage.

  1. Walsh RN, Cummins RA. The Open-Field Test: a critical review. Psychol Bull. 1976;83(3):482-504. doi:10.1037/0033-2909.83.3.482
  2. Prut L, Belzung C. The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: a review. Eur J Pharmacol. 2003;463(1-3):3-33. doi:10.1016/s0014-2999(03)01272-x
  3. Tatem KS, Quinn JL, Phadke A, Yu Q, Gordish-Dressman H, Nagaraju K. Behavioral and locomotor measurements using an open field activity monitoring system for skeletal muscle diseases. J Vis Exp. 2014;(91):51785. doi:10.3791/51785
  4. Crawley JN. Behavioral phenotyping of transgenic and knockout mice. Brain Res. 1999;835(1):18-26. doi:10.1016/s0006-8993(98)01258-x
  5. Tang X, Orchard SM, Sanford LD. Home cage activity and behavioral performance in inbred and hybrid mice. Behav Brain Res. 2002;136(2):555-569. doi:10.1016/s0166-4328(02)00228-0
Activity Cage
Activity Cage
$2,990.00
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