Primary Assay — Guinea Pig
Pup Distress / Separation
Cavia porcellus
Pup separation elicits whistle calls and a glucocorticoid stress response. The paradigm models maternal-attachment biology and developmental neuroendocrinology.

Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Cavia porcellus.
| Parameter | Unit | Description |
|---|---|---|
| Whistle rate during separation | calls/min | Distress signal |
| Cortisol response | fold change | HPA activation |
| Latency to first whistle | s | Distress onset |
| Recovery time on reunion | s | Affiliative response |
References
Citations for Pup Distress / Separation
- Hennessy MB, et al. (1995). PMID: 7610136
Compatible Equipment
Hardware for Guinea Pig Research
Audiometric Sound Booth
Behavioral hearing thresholds
Multi-Channel Vocal Recording
Whistle and call analysis
Open-Field Arena (Guinea-Pig Sized)
Activity and anxiety
Maternal Observation Cage
Sow-pup interaction
Pup Separation Chamber
Distress vocalization paradigm
Related Assays
Other Guinea Pig Primary Assays

13
Auditory Threshold (Behavioral)
Cavia porcellus
Operant audiometry measures absolute and frequency-difference thresholds across 100 Hz - 50 kHz. The guinea pig audiogra…

13
Vocal Communication
Cavia porcellus
Guinea pigs emit a structured repertoire (whistle, chut, drrr, purr, scream) tied to social and motivational contexts. C…

13
Open Field
Cavia porcellus
Guinea pigs in an open field freeze, vocalize, and explore peripheries. The assay measures locomotion, thigmotaxis, and …
Run Pup Distress / Separation on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your guinea pig facility.