Primary Assay — Honeybee
Proboscis Extension Reflex (PER) Conditioning
Apis mellifera
The gold-standard invertebrate associative learning paradigm. Bees learn to extend their proboscis to a conditioned odor stimulus, enabling precise measurement of acquisition, retention, and extinction of olfactory memories.
Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Apis mellifera.
| Parameter | Unit | Description |
|---|---|---|
| Acquisition rate | % per trial | Learning across CS-US pairings |
| Memory retention (1h/24h) | % responding | Short/long-term memory |
| Extinction rate | trials to criterion | Unlearning of CS-US association |
References
Citations for Proboscis Extension Reflex (PER) Conditioning
- Guo Y, et al. (2017). Proboscis Extension Reflex in Apis mellifera with Only One Antenna. Bio Protoc, 7(23), e2624. PMID: 34595292
- Giurfa M, et al. (2004). Associative mechanosensory conditioning of the proboscis extension reflex in honeybees. Learn Mem, 11(3), 294-302. PMID: 15169859
Compatible Equipment
Hardware for Honeybee Research
PER Conditioning Harness
Proboscis extension reflex assays
Hive Observation Camera
In-hive waggle dance recording
RFID Tracking System
Individual bee flight monitoring
Sucrose Stimulus Delivery
Gustatory response scoring
Video Arena
Defensive behavior testing
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Sucrose Responsiveness
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Run Proboscis Extension Reflex (PER) Conditioning on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your honeybee facility.
