Pass Rate (%)
Percentage of flies crossing the threshold line within the time limit, calculated per vial and averaged across replicates
ConductVision · Behavioral Analysis
Drosophila Negative Geotaxis
Automated scoring of climbing ability and motor performance in Drosophila.
DrosophilaMotor FunctionAuto Export
ConductVision / Drosophila Negative Geotaxis
Recording / Trial 3fly tracked
Pass Rate82%
Climb Speed2.1cm/s
PI0.78
Key Parameters
Metrics automatically extracted by ConductVision.
Climb Height
Maximum vertical position reached during the assay window, measured in millimeters from the vial base
Climb Velocity
Speed of upward movement after mechanical tap-down, computed as displacement over the first two seconds
Performance Index
Composite score integrating height reached, proportion passing, and climb velocity into a single 0–1 normalized metric
Failure Rate
Proportion of flies that fail to climb past the threshold within the assay period, stratified by genotype
Age-Dependent Decline
Longitudinal performance degradation across repeated trials, expressed as slope of PI vs. age in days
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Climb Latency
Time from tap-down to initiation of upward locomotion, sensitive to dopaminergic motor circuit deficits
Height Distribution
Population-level histogram of final positions binned by vial section, revealing bimodal motor phenotypes
Cohort Variability
Coefficient of variation across individuals within a genotype, indicating phenotypic penetrance and expressivity
Recovery After Repeat Tap
Performance change across consecutive tap-climb trials within a session, measuring motor fatigue and habituation
What is the Negative Geotaxis Assay?
The negative geotaxis (climbing) assay exploits the innate tendency of Drosophila to climb upward against gravity after being tapped to the bottom of a vial. Climbing performance declines with age, neurodegeneration, and motor-impairing mutations, making it a gold-standard assay for models of Parkinson's disease, ALS, and aging.
ConductVision automatically tracks vertical position across multiple flies simultaneously, computing pass/fail ratios, climb speed, and height reached. The software supports batch processing of hundreds of vials for large-scale genetic and drug screens with fully automated scoring.
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