ConductVision · Behavioral Analysis

Drosophila Courtship Assay

Automated quantification of courtship ritual behaviors and mating success.

DrosophilaReproductive BehaviorAuto Export
ConductVision / Drosophila Courtship Assay
Recording / Trial 3fly tracked
Courtship Index0.65
Wing Extension42s
Cop. Latency8.2min

Key Parameters

Metrics automatically extracted by ConductVision.

Courtship Index

Fraction of observation time spent performing any courtship sub-behavior, computed per 10-second sliding window

Wing Extension Duration

Cumulative time in unilateral wing vibration (courtship song), detected via wing-angle pose estimation

Following Time

Duration of oriented pursuit where the male heading aligns within 30° of the female bearing

Copulation Latency

Time from introduction of the pair to successful mounting, a key measure of male vigor and female receptivity

Copulation Duration

Length of the mating event from mounting to dismount, typically 15–20 minutes in wild-type Canton-S

Courtship Bout Frequency

Number of discrete courtship episodes separated by at least 2 seconds of non-courtship locomotion

+ 4 more parameters trackedShow all

Licking Events

Count of proboscis extension contacts with the female abdomen, a late-stage courtship behavior preceding mounting

Song Pulse / Sine Ratio

Relative proportion of pulse vs. sine song modes during wing vibration, reflecting species-specific courtship signals

Female Rejection Rate

Frequency of ovipositor extrusion, kicking, or decamping responses by the female during male courtship attempts

Inter-Fly Distance

Mean and minimum separation between male and female centroids across the session, indexing courtship engagement

What is the Drosophila Courtship Assay?

Drosophila courtship is a stereotyped multi-step ritual comprising orientation, following, wing extension (song production), licking, and attempted copulation. Courtship behavior integrates sensory processing, motor circuits, and motivational state, making it a powerful model for studying neural circuits, pheromone signaling, and sexual selection.

ConductVision tracks both male and female flies with identity preservation, automatically classifying courtship sub-behaviors using body orientation and wing angle detection. The software outputs courtship index, bout analysis, and latency metrics for genetic screens and neurobiology studies.

Ready to automate your behavioral analysis?

Request a demo or contact our team to discuss how ConductVision can accelerate your research.