ConductVision · Behavioral Analysis

Freezing Detection

Automated freezing bout detection for fear conditioning, PTSD, and anxiety research.

Built-inRodentAnxietyAuto Export
ConductVision / Freezing Detection
Recording / Trial 3subject tracked
Freezing Duration42%
Freezing Percentage18%
Bout Count8

Key Parameters

Metrics automatically extracted by ConductVision.

Freezing Duration

Total time spent immobile

Freezing Percentage

Percent of session in freezing state

Bout Count

Number of discrete freezing episodes

Mean Bout Length

Average duration per freezing episode

24.3s

Latency to First Freeze

Time from stimulus to first freeze

Freezing by Epoch

Freezing percentage in time bins

+ 2 more parameters trackedShow all

Movement Threshold

Pixel-change sensitivity for freeze/non-freeze boundary

Contextual vs Cued

Freezing in context A vs tone presentation

What is Freezing Detection?

Freezing — the complete cessation of movement except breathing — is the gold-standard measure of conditioned fear. It is central to fear conditioning paradigms, PTSD models, and anxiolytic drug screening, making automated freezing quantification essential for high-throughput behavioral neuroscience.

ConductVision detects freezing via two complementary methods: MOG2 background subtraction measuring pixel change below a calibrated threshold, and U-Net deep-learning segmentation for higher accuracy in complex backgrounds. Both methods run in real time during acquisition and produce frame-by-frame freezing classifications.

Research Applications

Fear Conditioning

  • Contextual fear conditioning — freezing in the training context
  • Cued fear conditioning — freezing to tone or light CS presentation
  • Fear extinction — decline in freezing over unreinforced CS trials
  • Fear renewal — return of freezing in a different context

Anxiety Research

  • Generalized anxiety models — baseline freezing in novel environments
  • PTSD models — enhanced freezing and extinction resistance
  • Anxiolytic drug screening — dose-response freezing reduction

Learning & Memory

  • Associative learning — CS-US pairing acquisition curves
  • Memory consolidation — post-training freezing retention
  • Reconsolidation — freezing after memory reactivation and disruption

Ready to automate your behavioral analysis?

Request a demo or contact our team to discuss how ConductVision can accelerate your research.