Microscopy Analysis Workflows
Specialized quantification pipelines for stereology, vascular imaging, neuron tracing, and spine morphology — all hardware-independent and AI-assisted.
Choose Your Workflow
Cell Counting & Stereology
Unbiased cell population estimates using the Optical Fractionator method with AI-assisted detection — without proprietary hardware. From $1,250/yr.
Vessel Reconstruction
3D vascular graph extraction from confocal and light-sheet microscopy with automated loop detection, diameter mapping, and terabyte-scale support.
Neuron Tracing
Semi-automated neuron reconstruction with branch detection, Sholl analysis, and morphometric exports compatible with SWC and NeuroMorpho.Org standards.
Spine Morphology
Dendritic spine detection and classification from confocal Z-stacks. Automatic spine head measurement, density quantification, and morphology categorization.
Cell Counting & Stereology
ConductVision Stereology brings design-based stereology to any image dataset. No motorized microscope, proprietary camera, or expensive control hardware required.
Traditional stereology systems require $15,000–$40,000 in perpetual licenses plus proprietary hardware. ConductVision Stereology works directly on your image files — SVS, NDPI, OME-TIFF, or any microscope format — starting at $1,250/yr.
Stereology Capabilities
Optical Fractionator
Define your region of interest and sampling grid. The software systematically samples the volume to estimate total population without counting every cell.
Human-in-the-Loop AI
The AI suggests candidate cells inside the counting frame. Researchers accept or reject detections, allowing the system to learn the definition of a valid cell marker.
Live Coefficient of Error
CE updates live as new sampling sites are counted. Stop once statistical confidence reaches your desired threshold — know when to stop counting.
True 3D Counting
Scroll through Z-stacks using a virtual disector probe. Only nuclei entering the counting frame are counted, eliminating lost-cap bias.
Stereology Workflow
- ConductPrep — Preprocess and stitch whole slide images or microscopy Z-stacks
- ROI Definition — Outline the anatomical region such as hippocampus or cortex
- Sample & Count — Run the AI-assisted Optical Fractionator on systematically sampled sites
- Statistical Report — Export total population (N), volume (V), and density estimates to Excel
Vessel Reconstruction
ConductVision Vessel extracts 3D vascular graphs from confocal and light-sheet microscopy. Compatible with iDISCO, CLARITY, and expansion microscopy.
Blood vessels form anastomosis loops that standard neuron tracers cannot handle. ConductVision Vessel automatically detects and reconstructs closed vascular loops with diameter-aware tracking along the entire vessel path.
Vessel Analysis Capabilities
Loop Detection
Automatic detection and reconstruction of closed vascular loops — anastomosis networks that cause standard neuron tracers to fail.
Diameter Mapping
Continuous diameter tracking along the entire vessel path to distinguish arteries, capillaries, and veins.
Terabyte-Scale Support
Cleared tissue datasets from light-sheet microscopy can exceed 1 TB. ConductVision Vessel processes large volumes block-by-block using the ConductPrep engine.
Graph Export
Export vascular graphs as SWC files, NetworkX graphs, or CSV tables with branch points, endpoints, and diameter measurements.
Neuron Tracing
Semi-automated neuron reconstruction for morphometric analysis. ConductVision Tracing detects neuronal branches, measures path lengths, and computes Sholl intersection profiles from confocal Z-stacks.
Exports are compatible with SWC format and NeuroMorpho.Org for cross-study comparison and open data sharing.
Spine Morphology
Automatic dendritic spine detection and classification from high-resolution confocal Z-stacks. ConductVision Spine measures spine head diameter, neck length, and spine density along dendritic segments.
Spine types (mushroom, thin, stubby, filopodia) are classified automatically with manual override capability for publication-quality data.
Gold Standard Quantification. Modern Pricing.
Conduct rigorous microscopy analysis without proprietary hardware. All workflows integrate with ConductPrep for seamless preprocessing.
