Wall Zone Time %
Proportion of time spent in the peripheral zone along arena walls
Quantify wall-hugging behavior as a measure of anxiety in zebrafish open-field arenas.
Metrics automatically extracted by ConductVision.
Proportion of time spent in the peripheral zone along arena walls
Proportion of time in the central open area
Number of crossings between peripheral and center zones
Swim distance broken down by peripheral vs. center region
Time to first enter the center zone after trial start
Mean swimming speed across zones
Thigmotaxis — the tendency to remain near the walls of an arena — is a robust anxiety-like behavior conserved from zebrafish to mammals. In circular or rectangular open-field arenas, anxious zebrafish spend significantly more time in the peripheral zone, while anxiolytic treatments increase center exploration.
ConductVision automatically defines peripheral and center zones, tracking zone occupancy, transitions, and swim kinematics per zone. The assay is widely used for anxiolytic compound screening, developmental neurotoxicology, and genetic models of anxiety disorders in both larvae and adult zebrafish.
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