ConductVision · Behavioral Analysis

Zebrafish Thigmotaxis

Quantify wall-hugging behavior as a measure of anxiety in zebrafish open-field arenas.

ZebrafishAnxietyAuto Export
ConductVision / Zebrafish Thigmotaxis
Recording / Trial 3fish tracked
Wall Zone Time %42%
Center Zone Time %27%
Zone Transitionsauto

Key Parameters

Metrics automatically extracted by ConductVision.

Wall Zone Time %

Proportion of time spent in the peripheral zone along arena walls

Center Zone Time %

Proportion of time in the central open area

Zone Transitions

Number of crossings between peripheral and center zones

Distance per Zone

Swim distance broken down by peripheral vs. center region

Latency to Center

Time to first enter the center zone after trial start

Velocity

Mean swimming speed across zones

What is Zebrafish Thigmotaxis?

Thigmotaxis — the tendency to remain near the walls of an arena — is a robust anxiety-like behavior conserved from zebrafish to mammals. In circular or rectangular open-field arenas, anxious zebrafish spend significantly more time in the peripheral zone, while anxiolytic treatments increase center exploration.

ConductVision automatically defines peripheral and center zones, tracking zone occupancy, transitions, and swim kinematics per zone. The assay is widely used for anxiolytic compound screening, developmental neurotoxicology, and genetic models of anxiety disorders in both larvae and adult zebrafish.

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