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Thermal Gradient
SKU CS-958331
$14,990.00
IncludesStandard care
Linear thermal gradient platform with 10 individually controlled zones (-5°C to +70°C) for assessing thermal preference and pain sensitivity in rodents across 20 temperature zones.
Scientist guidance
Louise Corscadden, PhD
Director of Science · ConductScience
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Key Specifications
Full details →- Model fit
- Mouse, Rat
- SKU family
- CS-958331
- Shipping box
- 120.0 x 10.0 x 15.0 cm
- Ordering
- Online checkout and quote request available
- Category
- Behavioral Mazes
- Build notes
- Aluminum alloy
The Thermal Gradient apparatus provides precise temperature control across a linear platform to assess thermal preference and pain sensitivity in rodents. The system features 10 individually controlled zones creating 20 total temperature zones (arranged in 2 rows of 10 adjacent plates) with temperature control ranging from -5°C to +70°C in 1-degree increments. The 120 cm aluminum alloy base plate ensures uniform heat distribution while the transparent PPC top cover allows for behavioral observation during testing.
This apparatus enables researchers to quantify thermal preference, thermal hyperalgesia, and cold allodynia responses by allowing animals to freely explore and select preferred temperatures along the gradient. The system integrates with video tracking software for automated behavioral analysis and includes data export capabilities with automatic calculation of statistical parameters for efficient data management.
How It Works
The thermal gradient test operates on the principle of free choice behavior, where animals can move along a temperature continuum to select their preferred thermal environment. The apparatus creates a linear thermal gradient through 10 independently controlled aluminum plates arranged in two parallel rows, generating 20 distinct temperature zones across the 120 cm platform length.
Each zone maintains precise temperature control within 1-degree accuracy, allowing researchers to establish gradients from hypothermic (-5°C) to nociceptive (70°C) ranges. Animals placed on the gradient naturally explore the platform and demonstrate preference by spending differential time in specific temperature zones. The aluminum alloy construction ensures rapid and uniform heat transfer, while the transparent top cover enables direct behavioral observation without thermal interference.
Behavioral responses are quantified by measuring time spent, distance traveled, and movement patterns within each temperature zone. The system integrates with video tracking software to automatically record position and calculate preference indices, thermal thresholds, and avoidance latencies for objective pain assessment.
Features & Benefits
10 individually controlled temperature zones
Enables precise gradient customization and independent zone temperature adjustment for flexible experimental design across thermal ranges
Temperature range -5°C to +70°C with 1-degree accuracy
Covers complete spectrum from hypothermic to nociceptive temperatures for comprehensive thermal sensitivity assessment
120 cm aluminum alloy base plate
Provides uniform heat distribution and sufficient platform length for natural exploratory behavior in rodents
Transparent PPC top cover
Allows direct behavioral observation while containing animals and preventing thermal interference from ambient environment
20 total temperature zones (2 rows of 10 plates)
Doubles available testing positions for higher throughput studies or simultaneous bilateral temperature exposure
Excel data export with automatic statistics
Streamlines data analysis workflow with automated calculation of mean, standard deviation, and preference indices
EthoVision XT compatibility
Enables automated tracking and analysis of movement patterns, zone preferences, and thermal threshold measurements
Grey PPC wall construction
Provides thermal insulation and visual containment while maintaining neutral color to minimize behavioral bias
Add-ons and modifications
| Add-on | Price | Details |
|---|---|---|
| Gradient Test | $14,990 | Length: 120 cm Width: 10 cm Height: 15 cm The test apparatus consists of a platform made up of 10 adjacent plates in 2 rows, allowing for 20 total temperature z |
Add any of these to your quote request.
Temperature Zones
- 10 zones for individual control
Total Zones
- 20 total temperature zones (10 adjacent plates in 2 rows)
Base Plate Material
- Aluminum alloy
Wall Material
- Grey PPC
Top Cover Material
- Transparent PPC
Data Export
- Excel export capability
Data Calculations
- Autocalculation of mean, std dev
Compatible Software
- Noldus EthoVision® XT video tracking system
Behavioral Construct
- thermal preference
- thermal hyperalgesia
- cold allodynia
- nociceptive behavior
- pain threshold
- thermal avoidance
- thermoregulation
Automation Level
- semi-automated
Material
- Aluminum alloy
- Grey PPC
- Transparent PPC
Color
- Grey
- Transparent
Temperature Range
- -5°C to +70°C
Accuracy
- 1 degree stepwise
Species
- Mouse
- Rat
Research Domain
- Addiction Research
- Behavioral Pharmacology
- Neurodegeneration
- Neuroscience
- Pain Research
- Toxicology
Compatible Tracking Software
- ConductVision
Shipping weight
- 6.06 lb
Shipping box
- L: 120.0 cm
- W: 10.0 cm
- H: 15.0 cm
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Number of Temperature Zones | 10 individually controlled zones creating 20 total testing positions | Basic models often provide 3-5 zones with fewer control options | Greater spatial resolution enables detection of subtle preference shifts and more precise thermal threshold determination |
| Temperature Range and Accuracy | -5°C to +70°C with 1-degree stepwise control | Limited models may offer narrower ranges or lower precision control | Comprehensive coverage from hypothermic to nociceptive ranges with high precision for detecting small threshold changes |
| Platform Length | 120 cm linear gradient | Shorter platforms may restrict natural exploratory behavior | Extended length accommodates natural movement patterns and reduces edge effects in behavioral analysis |
| Data Integration | Excel export with automatic statistical calculations and EthoVision XT compatibility | Manual data recording or limited software integration | Automated analysis reduces human error and accelerates data processing for high-throughput studies |
| Construction Materials | Aluminum alloy base with PPC walls and transparent cover | Basic models may use less efficient heat transfer materials | Optimal thermal conductivity ensures rapid temperature equilibration and uniform gradient establishment |
| Zone Configuration | Dual-row design with 20 total temperature zones | Single-row configurations with fewer testing positions | Higher throughput capability and bilateral testing options for increased experimental efficiency |
This thermal gradient system provides comprehensive temperature control with 10 independent zones across a 120 cm platform, offering superior spatial resolution and temperature precision compared to basic gradient systems. The dual-row configuration and advanced data integration capabilities support both high-throughput screening and detailed behavioral analysis workflows.
Pain Research
Quantifying thermal hyperalgesia and allodynia in chronic pain models by measuring temperature preference shifts and avoidance behaviors.
Behavioral Pharmacology
Evaluating analgesic efficacy by assessing changes in thermal preference patterns before and after drug administration.
Neuroscience
Investigating thermoregulatory behavior and temperature preference in neurological disease models and genetic mutations affecting thermal sensation.
Toxicology
Assessing neuropathic effects of chemical compounds by monitoring thermal sensitivity changes and temperature avoidance behaviors.
Neurodegeneration
Characterizing thermal sensation deficits in models of peripheral neuropathy and diabetic neuropathy through preference testing.
Addiction Research
Studying thermal dysregulation during withdrawal states and assessing thermal preference changes in substance use models.
Practical Tips
Calibration
Verify temperature accuracy using an independent digital thermometer at the center of each zone, as edge effects may cause slight temperature variations.
Why: Ensures precise thermal stimuli delivery for reproducible pain threshold measurements.
Maintenance
Clean the aluminum base plate weekly with mild soap solution and dry thoroughly to maintain optimal heat transfer properties.
Why: Buildup of organic residues can create thermal barriers affecting gradient uniformity.
Best Practices
Acclimate animals to the testing environment for 30 minutes before thermal testing to reduce stress-induced behavioral artifacts.
Why: Stress responses can confound thermal preference measurements and affect pain sensitivity.
Data Quality
Record ambient room temperature and humidity during each session as these factors can influence animal thermal preference behaviors.
Why: Environmental variables help explain variability in thermal preference data and improve result interpretation.
Troubleshooting
If temperature zones show uneven heating, check for loose connections at temperature sensor probes and ensure proper thermal contact.
Why: Poor sensor contact leads to inaccurate temperature feedback and unstable thermal gradients.
Safety
Use the transparent cover at all times during testing to prevent animal escape and protect against accidental contact with heated surfaces.
Why: Prevents injury to both animals and researchers while maintaining controlled testing conditions.
Best Practices
Randomize starting positions across multiple test sessions to prevent positional bias in thermal preference measurements.
Why: Animals may develop spatial preferences independent of temperature that confound thermal sensitivity data.
Calibration
Allow 45-60 minutes of warm-up time when switching from cold to hot temperature ranges to ensure complete thermal equilibration.
Why: Aluminum thermal mass requires adequate time to reach stable temperatures across large temperature changes.
Setup Guide
Unpack and Inspect
Remove the thermal gradient apparatus from packaging and inspect the aluminum base plate, PPC walls, and transparent cover for shipping damage. Verify all temperature control units are properly connected.
Environmental Placement
Position the apparatus on a stable, level surface in a temperature-controlled room away from air vents and direct lighting. Ensure adequate ventilation around the control units.
Power Connection
Connect the temperature control system to appropriate power supply and verify all 10 zones display proper temperature readings. Allow 30 minutes for thermal equilibration.
Temperature Calibration
Use an independent temperature probe to verify zone temperatures match controller displays. Adjust calibration settings if necessary to ensure 1-degree accuracy across all zones.
Gradient Configuration
Program desired temperature gradient profile across the 10 zones using the control interface. Common gradients range from 10°C to 50°C in 4-5 degree increments.
Video System Integration
Position overhead camera and configure tracking software to monitor the 120 cm x 10 cm test area. Set zone boundaries in software to match physical temperature zones.
Habituation Protocol
Run initial habituation sessions with test animals at neutral temperatures (22-25°C) to establish baseline movement patterns before thermal preference testing.
What’s in the Box
- Thermal gradient apparatus with aluminum base plate
- Temperature control system for 10 zones
- Grey PPC wall assembly
- Transparent PPC top cover
- Power supply and control cables
- Temperature calibration certificate (typical)
- User manual and protocol guide (typical)
- Data collection software (typical)
Warranty
ConductScience provides a standard 1-year manufacturer warranty covering defects in materials and workmanship, with technical support for setup, calibration, and troubleshooting. Extended warranty and service contracts are available for high-throughput research facilities.
Compliance
ARRIVE Guidelines 2.0Thermal gradient testing protocols commonly incorporate ARRIVE reporting standards for transparent documentation of experimental design and animal welfare considerations.
IASP Guidelines for Pain ResearchSupports nociceptive testing workflows governed by International Association for the Study of Pain recommendations for thermal pain assessment methodologies.
Guide for Care and Use of Laboratory AnimalsUsed in research facilities operating under NIH guidelines for appropriate environmental enrichment and behavioral assessment protocols.
Q
What temperature gradients are typically used for detecting thermal hyperalgesia in neuropathic pain models?
A
Standard protocols establish gradients from 10°C to 50°C in 4-5 degree increments, with hyperalgesic animals showing increased avoidance of temperatures above 35-40°C compared to controls. The 1-degree accuracy enables detection of subtle threshold shifts.
Q
How long should thermal equilibration time be before starting behavioral testing?
A
Allow minimum 30 minutes for complete thermal equilibration across all zones after setting temperatures. Verify stability with independent temperature measurements before introducing animals to ensure consistent thermal gradients.
Q
What video tracking parameters are most important for thermal preference analysis?
A
Key parameters include zone occupancy time, transition frequency between zones, and movement velocity within each temperature range. Configure 2-5 cm zone boundaries in tracking software to match physical temperature plate divisions.
Q
Can the system accommodate different rodent species and age groups?
A
The 120 cm length and 10 cm width accommodate mice, rats, and juvenile animals. Transparent top cover height of 15 cm prevents escape while allowing natural movement. Adjust test duration based on species mobility patterns.
Q
How frequently should temperature calibration be performed?
A
Perform monthly calibration verification using independent temperature probes at each zone. Daily verification of 2-3 key zones is recommended for studies requiring high precision thermal threshold measurements.
Q
What maintenance procedures are required for consistent performance?
A
Clean aluminum surfaces weekly with mild detergent to maintain heat transfer efficiency. Inspect temperature sensors quarterly and recalibrate annually or after any maintenance involving heating elements.
Q
How does this system compare to hot plate or cold plate testing methods?
A
Unlike binary choice tests, the thermal gradient provides continuous temperature selection allowing detection of subtle preference changes and establishment of thermal preference curves rather than simple threshold measurements.
Q
What statistical approaches are recommended for analyzing thermal preference data?
A
Use time-weighted temperature preference calculations and area-under-curve analysis for preference profiles. The system's Excel export includes automatic mean and standard deviation calculations for zone occupancy data.
Have a question about this product?
Track rodents with ConductVision
ConductVision is our video-tracking software. Record your sessions on video, and it scores standard rodent tests, such as those listed below.
Examples of tests it scores
To check whether ConductVision scores your protocol in this apparatus, ask in your quote request.
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Thermal Gradient
$14,990.00
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