Experimental Confounds
Threats to validity such as handling, lighting, odor, housing, habituation, and timing.
Method focus
These are the checks that make the page family useful when a scientist is choosing a method, endpoint, or analysis path.
Guides in this family
Use these guides to connect the scientific question to a measurable endpoint, defensible design choice, and reporting language.
Habituation
Habituation controls how novelty, handling, chamber exposure, and apparatus context affect behavioral readouts.
Lighting
Lighting changes visibility, aversion, arousal, and tracking quality, so illumination should be treated as a controlled method variable.
Handling
Handling can change stress, arousal, compliance, and task engagement, making it a protocol variable rather than a background detail.
Circadian phase
Circadian phase affects physiology, movement, arousal, and task engagement, so test timing should be planned and reported.
