Primary Assay — Mouse
Fear Conditioning (Contextual + Cued)
Mus musculus
Mouse learns association between a neutral context/tone (CS) and foot shock (US). Freezing behavior is the primary readout. Contextual FC is hippocampal-dependent; cued FC is amygdala-dependent.
Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Mus musculus.
| Parameter | Unit | Description |
|---|---|---|
| Freezing % (baseline) | % | Pre-conditioning spontaneous freezing |
| Freezing % (context test) | % | Freezing in conditioned context 24h later |
| Freezing % (cue test) | % | Freezing to auditory CS in novel context |
| Latency to freeze | s | Time from CS/context onset to first freeze |
| Fear generalization | ratio | Freezing in novel vs conditioned context |
References
Citations for Fear Conditioning (Contextual + Cued)
- Fanselow MS. (1980). Conditioned and unconditional components of post-shock freezing. Pavlov J Biol Sci, 15(4), 177-182. PMID: 7208128
- Maren S. (2001). Neurobiology of Pavlovian fear conditioning. Annu Rev Neurosci, 24, 897-931. PMID: 11520922
- Curzon P, Rustay NR, Bhowmik KB. (2009). Cued and contextual fear conditioning for rodents. In: Methods of Behavior Analysis in Neuroscience. CRC Press. PMID: 21204331
Compatible Equipment
Hardware for Mouse Research
Open Field Maze (various sizes)
OFT locomotion and anxiety
Elevated Plus Maze
Anxiety-like behavior
Morris Water Maze
Spatial learning and memory
Fear Conditioning Chamber
Contextual and cued FC
Rotarod (accelerating)
Motor coordination
Three-Chamber Social Interaction
Sociability testing
Y-Maze
Spontaneous alternation
Barnes Maze
Spatial learning (dry, less stressful)
Light-Dark Box
Anxiety-like behavior
Forced Swim Test Cylinder
Depressive-like behavior
Tail Suspension Apparatus
Depressive-like behavior
Grip Strength Meter
Neuromuscular function
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Run Fear Conditioning (Contextual + Cued) on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your mouse facility.
