Primary Assay — Zebrafish
Locomotion Profiling
Danio rerio
Baseline locomotor activity quantifies general motor function, neurological status, and drug effects. Measured in both larvae (96-well plates) and adults (open tank).
Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Danio rerio.
| Parameter | Unit | Description |
|---|---|---|
| Total distance | mm | Cumulative path length |
| Mean velocity | mm/s | Average speed over session |
| Maximum velocity | mm/s | Peak instantaneous speed |
| Angular velocity | °/s | Rate of directional change |
| Meandering | °/mm | Turning per unit distance |
| Immobility time | s | Total time below velocity threshold |
References
Citations for Locomotion Profiling
- Padilla S, Hunter DL, Padnos B, Frady S, MacPhail RC. (2011). Assessing locomotor activity in larval zebrafish: influence of extrinsic and intrinsic variables. Neurotoxicol Teratol, 33(6), 624-630. PMID: 21871562
- Ingebretson JJ, Bhatt DK. (2014). Quantitative behavioral analyses of larval zebrafish. J Vis Exp, (84), e50838. PMID: 24561567
Compatible Equipment
Hardware for Zebrafish Research
DanioVision Observation Chamber
Larval 96-well locomotion and light/dark assays
ZebraBox Multi-Well System
High-throughput larval screening
Zebrafish Behavioral Tank
Adult novel tank, shoaling, social preference
T-Maze for Zebrafish
Spatial learning and memory
Infrared Camera System
Dark-phase recording without light artifact
Related Assays
Other Zebrafish Primary Assays
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Light/Dark Challenge Assay
Danio rerio
Larval zebrafish exhibit robust locomotor changes when alternating between light and dark phases, with dark phases trigg…
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Thigmotaxis (Wall-Hugging)
Danio rerio
Larval and adult zebrafish display increased wall-proximity behavior in anxiogenic conditions, analogous to rodent thigm…
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Novel Tank Diving Test
Danio rerio
Adult zebrafish initially dive to the bottom of a novel tank and gradually explore upper regions. Bottom-dwelling time s…
Run Locomotion Profiling on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your zebrafish facility.
