Primary Assay — Zebrafish
Novel Tank Diving Test
Danio rerio
Adult zebrafish initially dive to the bottom of a novel tank and gradually explore upper regions. Bottom-dwelling time serves as an anxiety-like index, analogous to the rodent open field center avoidance.
Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Danio rerio.
| Parameter | Unit | Description |
|---|---|---|
| Time in bottom third | s | Duration in lower zone (anxiety proxy) |
| Latency to top | s | Time until first entry to upper third |
| Top-bottom transitions | count | Vertical exploration frequency |
| Erratic movements | count | Sharp directional changes >90° |
| Freezing bouts | count/s | Immobility episodes and total duration |
References
Citations for Novel Tank Diving Test
- Levin ED, Bencan Z, Cerutti DT. (2007). Anxiolytic effects of nicotine in zebrafish. Physiol Behav, 90(1), 54-58. PMID: 17049956
- Egan RJ, Bergner CL, Hart PC, et al. (2009). Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish. Behav Brain Res, 205(1), 38-44. PMID: 19540270
Compatible Equipment
Hardware for Zebrafish Research
DanioVision Observation Chamber
Larval 96-well locomotion and light/dark assays
ZebraBox Multi-Well System
High-throughput larval screening
Zebrafish Behavioral Tank
Adult novel tank, shoaling, social preference
T-Maze for Zebrafish
Spatial learning and memory
Infrared Camera System
Dark-phase recording without light artifact
Related Assays
Other Zebrafish Primary Assays
01
Light/Dark Challenge Assay
Danio rerio
Larval zebrafish exhibit robust locomotor changes when alternating between light and dark phases, with dark phases trigg…
01
Thigmotaxis (Wall-Hugging)
Danio rerio
Larval and adult zebrafish display increased wall-proximity behavior in anxiogenic conditions, analogous to rodent thigm…
01
Shoaling Assay
Danio rerio
Zebrafish are social species that form cohesive groups (shoals). Disrupted shoaling serves as a readout for social defic…
Run Novel Tank Diving Test on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your zebrafish facility.
