Primary Assay — Zebrafish
Thigmotaxis (Wall-Hugging)
Danio rerio
Larval and adult zebrafish display increased wall-proximity behavior in anxiogenic conditions, analogous to rodent thigmotaxis in open field tests. Attenuated by anxiolytics (diazepam), enhanced by anxiogenics (caffeine).
Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Danio rerio.
| Parameter | Unit | Description |
|---|---|---|
| % time in edge zone | % | Proportion of time within 1 body-length of wall |
| Wall proximity | mm | Mean distance to nearest wall |
| Center-edge transitions | count | Number of crossings between zones |
| Edge zone path length | mm | Distance traveled in peripheral zone |
| Anxiolytic response | Δ% | Change in edge time after drug treatment |
References
Citations for Thigmotaxis (Wall-Hugging)
- Schnörr SJ, Steenbergen PJ, Richardson MK, Champagne DL. (2012). Measuring thigmotaxis in larval zebrafish. Behav Brain Res, 228(2), 367-374. PMID: 22197677
- Champagne DL, Hoefnagels CC, de Kloet RE, Richardson MK. (2010). Translating rodent behavioral repertoire to zebrafish (Danio rerio): relevance for stress research. Behav Brain Res, 214(2), 332-342. PMID: 20540966
Compatible Equipment
Hardware for Zebrafish Research
DanioVision Observation Chamber
Larval 96-well locomotion and light/dark assays
ZebraBox Multi-Well System
High-throughput larval screening
Zebrafish Behavioral Tank
Adult novel tank, shoaling, social preference
T-Maze for Zebrafish
Spatial learning and memory
Infrared Camera System
Dark-phase recording without light artifact
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Shoaling Assay
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Run Thigmotaxis (Wall-Hugging) on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your zebrafish facility.
