Primary Assay — Zebrafish
Shoaling Assay
Danio rerio
Zebrafish are social species that form cohesive groups (shoals). Disrupted shoaling serves as a readout for social deficits, anxiogenic states, or neurotoxicity.
Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Danio rerio.
| Parameter | Unit | Description |
|---|---|---|
| Inter-individual distance | mm | Mean distance between all fish pairs |
| Nearest neighbor distance | mm | Distance to closest conspecific |
| Polarization | 0-1 | Alignment of swimming direction |
| Cohesion index | mm² | Area of convex hull around group |
| Shoal preference time | s | Time spent near shoal vs alone |
References
Citations for Shoaling Assay
- Miller N, Gerlai R. (2012). From schooling to shoaling: patterns of collective motion in zebrafish (Danio rerio). PLoS ONE, 7(11), e48865. PMID: 23155420
- Green J, Collins C, Kyzar EJ, et al. (2012). Automated high-throughput neurophenotyping of zebrafish social behavior. J Neurosci Methods, 210(2), 266-271. PMID: 22890236
Compatible Equipment
Hardware for Zebrafish Research
DanioVision Observation Chamber
Larval 96-well locomotion and light/dark assays
ZebraBox Multi-Well System
High-throughput larval screening
Zebrafish Behavioral Tank
Adult novel tank, shoaling, social preference
T-Maze for Zebrafish
Spatial learning and memory
Infrared Camera System
Dark-phase recording without light artifact
Related Assays
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Novel Tank Diving Test
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Run Shoaling Assay on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your zebrafish facility.
