ConductVision · Behavioral Analysis

Drosophila Circadian Activity

Monitor 24-hour activity/rest cycles and circadian rhythm parameters in Drosophila.

DrosophilaCircadian / SleepAuto Export
ConductVision / Drosophila Circadian Activity
LightDarkIRIR
Recording / Trial 3fly tracked
Period23.8h
Rhythm Str.4.2χ²
Anticipation0.68

Key Parameters

Metrics automatically extracted by ConductVision.

Activity Counts per Bin

Beam breaks or distance per configurable time interval (1-min to 60-min bins) rendered as actogram double-plots

Morning/Evening Anticipation

Activity ramp index in the 3 hours preceding lights-on and lights-off transitions, quantifying clock output

Free-Running Period

Endogenous circadian period under constant darkness (DD) or constant light (LL), computed via chi-squared periodogram

Phase Shift

Change in activity onset timing after light or temperature pulse perturbation, reported as advance or delay in hours

Rest Duration

Total quiescence time during subjective night using the 5-minute immobility threshold standard for Drosophila

Power Spectrum

Chi-squared periodogram amplitude at the dominant frequency, indicating strength and robustness of circadian rhythmicity

+ 4 more parameters trackedShow all

Rhythm Strength (RS)

Ratio of periodogram peak amplitude to significance threshold, classifying flies as rhythmic, weakly rhythmic, or arrhythmic

Daytime / Nighttime Activity Ratio

Total activity in light phase divided by dark phase, revealing diurnal preference and clock-controlled activity partitioning

Sleep Bout Number

Count of consolidated quiescence episodes per 12-hour phase, distinguishing fragmented from consolidated rest patterns

Entrainment Speed

Number of cycles required to re-synchronize after a phase shift (jet-lag protocol), measuring clock plasticity

What is Drosophila Circadian Activity Monitoring?

Drosophila is one of the premier genetic model organisms for circadian biology. The fly circadian clock — driven by period, timeless, and cryptochrome genes — produces robust ~24-hour activity/rest rhythms that are highly sensitive to genetic disruption and pharmacological modulation. Drosophila Activity Monitors (DAM) have historically relied on IR beam breaks, but video tracking provides richer locomotor data.

ConductVision replaces or complements traditional DAM systems with continuous video-based tracking, generating activity counts, actograms, and periodograms. The software supports LD, DD, and LL protocols with configurable light schedules for phase response, jet-lag, and social synchronization studies.

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